A5007914982- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Analytical Chemistry and Chromatography
- Microtubule and mitosis dynamics
- Cancer, Lipids, and Metabolism
- Neuroscience and Neuropharmacology Research
- Ion-surface interactions and analysis
- Ubiquitin and proteasome pathways
- Cellular transport and secretion
- Mitochondrial Function and Pathology
- Lipid metabolism and biosynthesis
- Lipid Membrane Structure and Behavior
- Peroxisome Proliferator-Activated Receptors
- Glycosylation and Glycoproteins Research
- Cancer, Hypoxia, and Metabolism
- Genetic and Kidney Cyst Diseases
- Adipose Tissue and Metabolism
- Genetics and Neurodevelopmental Disorders
- Sirtuins and Resveratrol in Medicine
- Fatty Acid Research and Health
- Amino Acid Enzymes and Metabolism
- RNA Research and Splicing
- Protist diversity and phylogeny
- Asthma and respiratory diseases
Hamamatsu University School of Medicine
2016-2025
University of Hong Kong
2014-2023
Hamamatsu Photonics (Japan)
2017-2023
Imaging Center
2016-2022
Photonics (United States)
2018-2021
Chinese University of Hong Kong
2016-2020
Cellular Research (United States)
2020
RIKEN Center for Computational Science
2016-2018
Japan Agency for Medical Research and Development
2017
National Institute for Physiological Sciences
2006-2017
Only a small proportion of the mouse genome is transcribed into mature messenger RNA transcripts. There an international collaborative effort to identify all full-length mRNA transcripts from mouse, and ensure that each represented in physical collection clones. Here we report manual annotation 60,770 complementary DNA sequences. These are clustered 33,409 'transcriptional units', contributing 90.1% newly established transcriptome database. Of these transcriptional units, 4,258 new...
Experiments with vesicles containing N-methyl-D-aspartate (NMDA) receptor 2B (NR2B subunit) show that they are transported along microtubules by KIF17, a neuron-specific molecular motor in neuronal dendrites. Selective transport is accomplished direct interaction of the KIF17 tail PDZ domain mLin-10 (Mint1/X11), which constituent large protein complex including mLin-2 (CASK), mLin-7 (MALS/Velis), and NR2B subunit. This interaction, specific for neurotransmitter critically important...
Microtubules function as molecular tracks along which motor proteins transport a variety of cargo to discrete destinations within the cell. The carboxyl termini alpha- and beta-tubulin can undergo different posttranslational modifications, including polyglutamylation, is particularly abundant mammalian nervous system. Thus, this modification could serve "traffic sign" for in neuronal cells. To investigate whether polyglutamylated alpha-tubulin perform function, we analyzed ROSA22 mice that...
KIF17, a recently characterized member of the kinesin superfamily proteins, has been proposed to bind in vitro protein complex containing mLin10 (Mint1/X11) and NR2B subunit NMDA receptors (NMDARs). In mammalian brain, NMDARs play an important role synaptic plasticity, learning, memory. Here we present, for first time, dynamic properties KIF17 provide evidence its function transport living neurons. vesicles enter move specifically along dendrites processive way, at average speed 0.76...
Statins (3-hydroxy-3-methylglutaryl-coenzyme A reductase inhibitors) have pleiotropic vascular protective effects besides cholesterol lowering. Recently, experimental and clinical studies indicated that senescence of endothelial cells is involved in dysfunction atherogenesis. Therefore, the present study was performed to determine whether statins would reduce clarify molecular mechanisms underlying antisenescent property statins.Senescent human umbilical vein were induced by hydrogen...
Previous studies have shown that MALDI-imaging mass spectrometry (IMS) can be used to visualize the distribution of various biomolecules, especially lipids, in cells and tissues. In this study, we report cell-selective PUFA-containing glycerophospholipids (GPLs) mouse brain. We established a practical experimental procedure for IMS GPLs. demonstrated optimization composition matrix solution spectrum normalization total ion current (TIC) is critical. Using our procedure, simultaneously...
Today, two-dimensional mass spectrometry analysis of biological tissues by means a technique called imaging, imaging (MSI), or (IMS) has found application in investigating the distribution molecules—MSI with matrix-assisted laser desorption/ionization (MALDI) and secondary ion MS (SIMS). However, size matrix crystal migration analytes can decrease spatial resolution MALDI, SIMS only ionize compounds relatively low molecular weights. To overcome these problems, we developed...
Imaging mass spectrometry is becoming a popular visualization technique in the medical and biological sciences. For its continued development, ability to both visualize identify molecules directly on tissue surface using tandem (MSn) essential. We established an imaging system based matrix-assisted laser/desorption ionization quadrupole ion trap time-of-flight type instrument (AXIMA-QIT, Shimadzu, Kyoto, Japan), which was compatible with highly sensitive MSn. In this paper, we present...
Airway epithelial cilia protect the mammalian respiratory system from harmful inhaled materials by providing force necessary for effective mucociliary clearance. Ciliary beating is asymmetric, composed of clearly distinguished and recovery strokes. Neither importance nor essential components responsible asymmetry has been directly elucidated. We report here that crucial ciliary function requires tubulin glutamylation, a unique posttranslational modification highly abundant in cilia. WT...
Gangliosides are particularly abundant in the central nervous system (CNS) and thought to play important roles memory formation, neuritogenesis, synaptic transmission, other neural functions. Although several molecular species of gangliosides have been characterized their individual functions elucidated, differential distribution CNS not well understood. In particular, whether different show patterns brain remains unclear. We report distinct characteristic distributions ganglioside species,...
We have developed a mass microscope (mass spectrometry imager with spatial resolution higher than the naked eye) equipped an atmospheric pressure ion-source chamber for laser desorption/ionization (AP-LDI) and quadrupole ion trap time-of-flight (QIT-TOF) analyzer. The optical combined spectrometer permitted us to precisely determine relevant tissue region prior performing imaging (IMS). An ultraviolet tightly focused triplet lens was used achieve high resolution. enables analyze fresh...
Breast cancer is the leading cause of and mortality in women worldwide. Recent studies have argued that there a close relationship between lipid synthesis progression because some enzymes related to are overexpressed breast tissues. However, distribution tissues has not been investigated. We aimed visualize phosphatidylcholines (PCs) lysoPCs (LPCs) human by performing matrix assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS), which novel technique enables...
Abstract Rice is one of the most important food crops in world and new varieties have been bred for specific purposes, such as development drought‐resistance, or enrichment functional factors. The localization composition metabolites must be investigated because all artificial interventions are expected to change rice. Imaging mass spectrometry using matrix‐assisted laser desorption/ionization (MALDI‐IMS) a suitable tool investigating metabolites; however, methodologies MALDI‐IMS analysis...