A5030469755- Neurogenesis and neuroplasticity mechanisms
- Mitochondrial Function and Pathology
- Anesthesia and Neurotoxicity Research
- Epigenetics and DNA Methylation
- Neuroscience and Neuropharmacology Research
- Reproductive System and Pregnancy
- Effects and risks of endocrine disrupting chemicals
- Nerve injury and regeneration
- Endometriosis Research and Treatment
- Microtubule and mitosis dynamics
- RNA modifications and cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Glioma Diagnosis and Treatment
- Nitric Oxide and Endothelin Effects
- Uterine Myomas and Treatments
- Neuroscience of respiration and sleep
- RNA Interference and Gene Delivery
- Effects of Radiation Exposure
- Virus-based gene therapy research
- Alzheimer's disease research and treatments
- Connexins and lens biology
- Immune Cell Function and Interaction
- RNA Research and Splicing
- Estrogen and related hormone effects
- Cell death mechanisms and regulation
Kyoto Prefectural University of Medicine
2012-2023
Kyoto Medical Center
2017
RELX Group (United States)
2012
RELX Group (United Kingdom)
2012
Kyoto University
1998-2007
Dynamic Imaging (United Kingdom)
2007
Miyazaki Prefectural Hospital
2004
Kagoshima University
2004
University of British Columbia
2003
Kyoto Pharmaceutical University
2003
To determine whether local estrogen production takes place in endometriotic or adenomyotic tissues, eutopic endometrium from patients with endometriosis adenomyosis, and normal endometrium, tissue specimens were examined by immunohistochemistry, catalytic activity, mRNA expression for aromatase cytochrome P450 (P450arom). P4500arom was immunohistochemically localized the cytoplasm of glandular cells respective diseases, whereas receptors progesterone nuclei stroma. Aromatase activity...
The stratum corneum of the skin serves as an effective barrier for maintenance internal milieu against external environment. At cell periphery is envelope, a highly insoluble membranous structure composed precursor proteins cross-linked by ɛ-(γ-glutamyl)lysine bonds. Transglutaminase 1 (TGase 1; keratinocyte TGase), membrane-bound isozyme TGase family, has been proposed to catalyze this process assembly. Deficient cross-linking envelope in some patients with autosomal recessive disorder...
A large number of natural killer (NK) cells appear in human uterine mucosa during the secretory phase and first trimester pregnancy. We investigated expression interleukin (IL)-15, a possible stimulator for these NK cells, endometrium decidua. Semiquantitative reverse transcriptase-polymerase chain reaction revealed that IL-15 mRNA was stronger pregnancy than proliferative phase. Immunohistochemistry immunoreactivity anti-IL-15 higher it This prominent perivascular stromal around invading...
Background and Purpose— Astrocytes may play a vital role in neuroprotection by providing energy substrates to neurons regulating the concentration of K + neurotransmitters through gap junctions. Connexin 43 (Cx43) is one major junction proteins astrocytes. We have shown that, after focal stroke, heterozygote Cx43 null (Cx43+/−) mice exhibited larger infarction volumes than wild-type (Cx43+/+) mice. explored underlying mechanism which junctional intercellular communication influences...
Abstract We have developed a new genome scanning method (restriction landmark genomic (RLGS)), based on the concept of using restriction enzyme sites as landmarks. RLGS employs direct end labeling DNA digested with and two‐dimensional electrophoresis high‐resolution. Its advantages are: (i) high‐speed ability, allowing simultaneous thousands landmarks; (ii) extension field different kinds landmarks in an additional series electrophoresis; (iii) application to any type organism because...
Abstract Bisphenol A (BPA) has been shown to disrupt thyroid hormone function. We therefore studied whether prenatal exposure low‐doses of BPA affects the morphology and expression some genes related brain development in murine fetal neocortex. Pregnant mice were injected subcutaneously with 20 μg/kg daily from embryonic day 0 (E0). Control animals received vehicle alone. For evaluating cell proliferation, neuronal differentiation migration, bromodeoxyuridine (BrdU) was intraperitoneally...
Adipose-derived stem cells (ADSCs) are attracting increased attention as a novel source in regenerative medicine. Transplantation of ADSCs promotes functional recovery animal models peripheral nerve injury, but the mechanism enhanced regeneration remains to be elucidated. In addition, it is important examine whether supportive functions dependent on donor age or anatomic site origin. this study, we examined effects factors produced by mouse Schwann (SCs) and dorsal root ganglion (DRG)...
Objectives: The present study examined the possibility that intracellular ATP levels dictate whether hypoxic cardiac myocytes die by apoptosis or necrosis. Background: Although and necrosis may appear to be distinct forms of cell death, recent studies suggest two represent different outcomes a common pathway. In ischemic myocardium, appears early, while energy stores are presumably still available, followed only later Methods: Neonatal rat were exposed continuous hypoxia, during which...
In order to address an age-dependent alteration in the concentration of beta-amyloid polypeptides (Abetas) within central nervous system and its probable predisposition amyloidgenesis Alzheimer's disease (AD), we measured two species soluble Abetas, Abeta40 Abeta42, cerebrospinal fluids (CSF) from randomly selected Japanese control subjects at various ages (n = 33) then compared these data with those AD patients 23). CSF concentrations Abeta42 peptides were (ANOVA, Bonferroni's multiple...
Abstract Monoclonal M6 antibody binds to the surface of murine central nervous system neurons as well apical surfaces epithelial cells in choroid plexus and proximal tubules kidney. antigen is expressed early embryonic day 10, most strongly marginal zone neural tube, remains detectable adulthood. IgG or Fab fragments interfere with extension neurites by cultured cerebellar neurons. Effects on neurite are readily after 24 h. No reduction cell viability detected during first 3 days treatment....
Restriction landmark genomic scanning using methylation-sensitive endonucleases (RLGS-M) is a newly developed powerful method for systematic detection of DNA methylation. Using this method, we scanned mouse brain DNAs from various developmental stages to detect the transcriptionally active regions. This approach based on assumption that CpG methylation, particularly islands, might be associated with gene transcriptional regulation. Genomic were prepared telencephalons 9.5-, 13.5- and...
To identify a neural phenotype in connexin43 null mutant mice, electrophysiological properties, intercellular communication and neuronal migration were studied the developing neocortex. In acute slice preparations from newborn characteristics of cortical hippocampal neurons not significantly different between wild type mice. However, gap junctional coupling as assessed by fluorescence recovery after photobleaching was attenuated neocortical brain slices assess migration, dividing cells...
Previous reports have demonstrated that some focal brain injuries increase amyloid precursor protein (APP) immunoreactivity in the region surrounding injury where it was localized, damaged axons and pre-alpha 2 cells of entorhinal cortex. However, to date, APP expression hippocampus remote from impact site has not been comprehensively studied. Therefore, we evaluated only locally injured cerebral cortex but also site. In present paper, diffuse axonal induced rats midline fluid percussion...