A5040530002- Epigenetics and DNA Methylation
- Genomic variations and chromosomal abnormalities
- Microtubule and mitosis dynamics
- Genomics and Phylogenetic Studies
- Parkinson's Disease Mechanisms and Treatments
- Genomics and Chromatin Dynamics
- Genetic Syndromes and Imprinting
- Neurological disorders and treatments
- Cancer Genomics and Diagnostics
- GABA and Rice Research
- Molecular Biology Techniques and Applications
- Gene expression and cancer classification
- Nerve injury and regeneration
- CRISPR and Genetic Engineering
- Prenatal Screening and Diagnostics
- Salivary Gland Tumors Diagnosis and Treatment
- Animal Genetics and Reproduction
- Mitochondrial Function and Pathology
- Metastasis and carcinoma case studies
- Chromosomal and Genetic Variations
- RNA modifications and cancer
- Neurogenesis and neuroplasticity mechanisms
- DNA and Biological Computing
- RNA Interference and Gene Delivery
- Lysosomal Storage Disorders Research
Osaka Metropolitan University
2022-2023
Osaka City University
2017-2020
National Human Genome Research Institute
1998
National Institutes of Health
1998
RIKEN BioResource Research Center
1994-1996
Roswell Park Comprehensive Cancer Center
1996
RIKEN
1994-1996
National Cerebral and Cardiovascular Center
1991-1995
Genetic Resources Center
1995
Kyoto Prefectural University of Medicine
1994
We have developed a powerful genomic scanning method, termed "restriction landmark scanning," that is useful for analysis of the DNA higher organisms using restriction sites as landmarks. Genomic radioactively labeled at cleavage specific rare cleaving enzyme and then size-fractionated in one dimension. The fractionated further digested with another more frequently occurring separated second This procedure gives two-dimensional pattern thousands scattered spots corresponding to first enzyme,...
Abstract We have developed a new genome scanning method (restriction landmark genomic (RLGS)), based on the concept of using restriction enzyme sites as landmarks. RLGS employs direct end labeling DNA digested with and two‐dimensional electrophoresis high‐resolution. Its advantages are: (i) high‐speed ability, allowing simultaneous thousands landmarks; (ii) extension field different kinds landmarks in an additional series electrophoresis; (iii) application to any type organism because...
Restriction landmark genomic scanning using methylation-sensitive endonucleases (RLGS-M) is a newly developed powerful method for systematic detection of DNA methylation. Using this method, we scanned mouse brain DNAs from various developmental stages to detect the transcriptionally active regions. This approach based on assumption that CpG methylation, particularly islands, might be associated with gene transcriptional regulation. Genomic were prepared telencephalons 9.5-, 13.5- and...
Abstract We have developed a multiplex method of genome analysis, restriction landmark genomic scanning (RLGS) that has been used to construct genetic maps in mice. Restriction landmarks are end-labeled fragments DNA separated by using high resolution, two-dimensional gel electrophoresis identifying as many two thousand loci single gel. Variation for several hundred these identified between laboratory strains and Mus spretus. The segregation more than 1100 RLGS analyzed recombinant inbred...
Although α-synuclein (αSyn) has been linked to Parkinson's disease (PD), the mechanisms underlying causative role in PD remain unclear. We previously proposed a model for transportable microtubule (tMT), which dynein is anchored short tMT by LIS1 followed kinesin-dependent anterograde transport; however that produce tMTs have not determined. Our vitro investigations of (MT) dynamics revealed αSyn facilitates formation MTs and preferentially binds carrying 14 protofilaments (pfs). Live-cell...
Abstract Parkinson’s disease is a progressive neurodegenerative disorder characterized by the preferential loss of tyrosine hydroxylase (TH)-expressing dopaminergic neurons in substantia nigra. Although abnormal accumulation and aggregation α-synuclein have been implicated pathogenesis disease, underlying mechanisms remain largely elusive. Here, we found that TH converts Tyr136 into dihydroxyphenylalanine (DOPA; Y136DOPA) through mass spectrometric analysis. Y136DOPA modification was clearly...
Restriction landmark genomic scanning (RLGS) is a novel method which enables us to simultaneously visualize large number of loci as two-dimensional gel spots. By this method, the status DNA methylation can efficiently be determined by monitoring appearance or disappearance spots using methylation-sensitive restriction enzyme. In present study, RLGS with NotI, we examined, in comparison brain profile, more than 900 among three types mouse cell lines: embryonal carcinoma line P19, stable...
Restriction landmark genomic scanning (RLGS) is a novel method which enables us to simultaneously visualize large number of loci as two-dimensional gel spots. By this method, the status DNA methylation can efficiently be determined by monitoring appearance or disappearance spots using methylation-sensitive restriction enzyme. In present study, RLGS with NotI, we examined, in comparison brain profile, more than 900 among three types mouse cell lines: embryonal carcinoma line P19, stable...
The field of genome editing was founded on the establishment methods, such as clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated protein (CRISPR/Cas) system, used to target DNA double-strand breaks (DSBs). However, efficiency also largely depends endogenous cellular repair machinery. Here, we report that specific modulation targeting vectors provide 3' overhangs at both ends increased homology-directed (HDR) in embryonic stem cells. We applied modulated...
Abstract The DNA methylation status of a large number genomic loci is visualized simultaneously and quantitatively as two‐dimensional gel spots in the newly developed restriction landmark scanning with methylation‐sensitive enzyme (RLGS‐M). Here, we demonstrate that RLGS‐M using Not I could also scan gene mammalian genomes, since almost all corresponding to randomly chosen were located near or transcriptional units (6 out 7 I‐linking clones) when mouse brain was used. This supports previous...
Alpha-synuclein (αSyn) and tau are abundant multifunctional neuronal proteins, their intracellular deposits have been linked to many neurodegenerative diseases, including Alzheimer9s disease Parkinson9s disease. Despite the relevance, physiological roles remain elusive, as mice with knock-out of either these genes do not exhibit overt phenotypes. To reveal functional cooperation, we generated αSyn<sup>−/−</sup>tau<sup>−/−</sup> double-knock-out characterized cross talk between proteins...