A5085159499- Reproductive System and Pregnancy
- Endometriosis Research and Treatment
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Pregnancy and Medication Impact
- Gynecological conditions and treatments
- Ovarian function and disorders
- Immune Cell Function and Interaction
- Uterine Myomas and Treatments
- Testicular diseases and treatments
- Sexual Differentiation and Disorders
- Reproductive Health and Technologies
- Reproductive Health and Contraception
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Assisted Reproductive Technology and Twin Pregnancy
- Reproductive Physiology in Livestock
- Prenatal Screening and Diagnostics
- Pregnancy and preeclampsia studies
- Endometrial and Cervical Cancer Treatments
- Neurogenesis and neuroplasticity mechanisms
- Proteoglycans and glycosaminoglycans research
- Consumer Perception and Purchasing Behavior
- Apelin-related biomedical research
- Cell Adhesion Molecules Research
- Male Reproductive Health Studies
Kyoto Katsura Hospital
2023-2024
Osaka Nishi Clinic
2012-2021
Kansai Medical University
2009-2018
Kyoto Prefectural University of Medicine
2004-2016
The aim of this prospective study was to investigate the prevalence chronic endometritis (CE) in infertile women with a history repeated implantation failure (RIF) and determine whether oral antibiotic treatment improves their live birth rate following embryo transfer (ET) cycles.Endometrial biopsy samples obtained from RIF were subjected immunohistochemistrical/histopathologic diagnosis CE. Following administration RIF/CE group, histopathologic cure rate, microbial detection reproductive...
A large number of natural killer (NK) cells appear in human uterine mucosa during the secretory phase and first trimester pregnancy. We investigated expression interleukin (IL)-15, a possible stimulator for these NK cells, endometrium decidua. Semiquantitative reverse transcriptase-polymerase chain reaction revealed that IL-15 mRNA was stronger pregnancy than proliferative phase. Immunohistochemistry immunoreactivity anti-IL-15 higher it This prominent perivascular stromal around invading...
Citation Kitaya K, Yasuo T. Immunohistochemistrical and clinicopathological characterization of chronic endometritis. Am J Reprod Immunol 2011; 66: 410–415 Problem Chronic endometritis is an elusive entity that often asymptomatic undetectable by conventional endometrial biopsy histological examination. Using immunohistochemistry for full-thickness endometrium, we sought its features. Method Study Two hundred thirty-four archival specimens obtained hysterectomy were immunostained the...
Studies suggest that persisting intrauterine bacterial infectious conditions such as chronic endometritis potentially impair the embryo implantation process. The microbial environment in female reproductive tract, however, remains largely undetermined infertile patients with a history of repeated failure (RIF). Using next-generation sequencing, we aimed to characterize microbiota endometrial fluid (EF) and vaginal secretions (VS) women RIF. Twenty-eight RIF eighteen undergoing first vitro...
Is the endometrial mononuclear cell population in infertile patients altered subjects with classical polyps (macropolyps) versus micropolyps that are hysteroscopically recognized as small uterine cavity protrusions? Macropolypoid endometrium had a low density of pan-leukocytes, pan-T cells and natural killer (NK) cells, whereas micropolypoid was characterized by high B plasmacytes, along NK cells. Endometrial co-exist at rate chronic endometritis, which is an unusual plasmacyte infiltration...
Problem Chronic endometritis ( CE ) is a local inflammatory condition with unusual plasmacyte infiltration in the endometrial stromal area. frequently found infertile women repeated implantation failure RIF ). In this study, we comprehensively investigated immunoglobulin (Ig) subclass expression suffering from versus without . Method of study Endometrial biopsy specimens obtained 28 and (the ‐ group), 23 but ‐non‐ 22 proven fertile undergoing hysterectomy for benign pathology control group)...
A large number of CD16(-) natural killer (NK) cells appear in the human endometrium after ovulation. One possible explanation for this phenomenon is recruitment from peripheral blood (PB) NK cells. In study, we examined whether IL-15 involved postovulatory PB The receptor alpha chain was expressed on but not CD16(+) an vitro migration assay, recombinant enriched Endometrial soluble protein fraction secretory phase, proliferative also these Neutralization phase with anti-IL-15 monoclonal...
Chronic endometritis (CE) is an unusual endometrial inflammation characterized by stromal plasmacyte infiltration. CE easily missed due to its subtle symptoms and demanding histopathological examinations. Although the immunohistochemistry for marker CD138 has facilitated detection of plasmacytes, accuracy biases this method diagnosis remain poorly understood. The aim study was investigate inter‑ intra‑observer variability in immunohistochemical plasmacytes human endometrium. A total 80 20...
Human endometrium is infiltrated by natural killer (NK) cells throughout the menstrual cycle. The number of endometrial NK low in proliferative phase, but acutely increases after ovulation, and reaches a peak late secretory suggesting that recruits these leukocytes selectively from circulating peripheral blood. We investigated expression macrophage inflammatory protein (MIP)-1β, potential chemoattractant for cells, endometrium. RT-PCR ELISA revealed MIP-1β expressed cycle at both message...
In this study, we investigated the expression of ligands for CXCR3 (Mig, IP-10, and I-TAC) CXCR4 (SDF-1) in human endometrium throughout menstrual cycle. By immunohistochemistry, immunostaining Mig IP-10 was found surface epithelia, glandular stroma with some cycle-dependent fluctuation. contrast, I-TAC or SDF-1 not detected. ELISA demonstrated that concentrations were higher secretory phase than proliferative phase, but SDF-1α detected only a few samples. Endometrial strongly correlated...
Chronic endometritis (CE) is an infectious and inflammatory disorder associated with infertility of unknown etiology, repeated implantation failure, recurrent pregnancy loss. In the current clinical practice, intrauterine interventions such as endometrial biopsy/histopathologic examinations and/or hysteroscopy are required for diagnosis CE. this study, we analyzed microbiota in vaginal secretions (VS) a potential prediction tool CE infertile women.Using next-generation sequencing analysis,...
Interferon (IFN)-γ plays a critical role in murine uterine spiral artery remodeling for successful pregnancy. The effect of IFN-γ on human microvasculature, however, remains poorly understood. aim this study was to identify the genes regulated by microvascular endothelial cells. gene expression profile cells evaluated cDNA microarray analysis and quantitative real-time reverse transcriptase-polymerase chain reaction selected interest. In vivo protein encoded some these Western blotting...