A5030113807- Microtubule and mitosis dynamics
- Cellular transport and secretion
- Micro and Nano Robotics
- Cellular Mechanics and Interactions
- Nuclear Structure and Function
- 14-3-3 protein interactions
- Advanced Materials and Mechanics
- Photosynthetic Processes and Mechanisms
- Glycosylation and Glycoproteins Research
- Cardiomyopathy and Myosin Studies
- Mitochondrial Function and Pathology
- Orbital Angular Momentum in Optics
- Microfluidic and Bio-sensing Technologies
- Barrier Structure and Function Studies
- Lysosomal Storage Disorders Research
- Epigenetics and DNA Methylation
- Erythrocyte Function and Pathophysiology
- Carbohydrate Chemistry and Synthesis
- Caveolin-1 and cellular processes
- Hippo pathway signaling and YAP/TAZ
- Galectins and Cancer Biology
- Protist diversity and phylogeny
- Molecular Communication and Nanonetworks
- Hedgehog Signaling Pathway Studies
- Metabolism, Diabetes, and Cancer
National Institute of Genetics
2019-2025
The Graduate University for Advanced Studies, SOKENDAI
2018-2025
National Institute of Information and Communications Technology
2016-2019
The University of Tokyo
2007-2014
Tokyo University of the Arts
2007-2014
Cytoplasmic streaming is driven by molecular motors that move along the cytoskeleton and entrain surrounding fluid. In certain cell types, direction of cytoplasmic not predefined but rather stochastic. For example, in meiotic zygotes, swirl reverses over time [Kimura , ]. While a mean-field theory explained reversal, structure flows three-dimensional space mechanical role endoplasmic reticulum (ER) remains unknown. To test hypothesis elastic ER bonds hydrodynamic interactions between...
LIS1 and NDEL1 are known to be essential for the activity of cytoplasmic dynein in living cells. We previously reported that directly regulated motility an vitro assay. suppressed inhibited translocation microtubules (MTs), while dissociated from MTs restored following suppression by LIS1. However, molecular mechanisms detailed interactions dynein, LIS1, remain unknown. In this study, we dissected regulatory effects on using full-length or truncated recombinant fragments NDEL1. The...
Human mutations in KATNB1 (p80) cause severe congenital cortical malformations, which encompass the clinical features of both microcephaly and lissencephaly. Although p80 plays critical roles during brain development, underlying mechanisms remain predominately unknown. Here, we demonstrate that regulates microtubule (MT) remodeling combination with NuMA (nuclear mitotic apparatus protein) cytoplasmic dynein. We show shuttles between nucleus spindle pole synchrony cell cycle. Interestingly,...
Summary Inside cells, molecular motors transport cargoes within the actively fluctuating environment known as cytoplasm. How fluctuations in cytoplasm affect motor-driven is not fully understood. In this study, we investigated role of for along microtubules using C. elegans early embryos, focusing on driven by cytoplasmic dynein. An artificial motor-cargo complex showed faster vivo than vitro , suggesting an acceleration mechanism. We also found that endogenous endosome dynein significantly...
Mechanical stress significantly affects the physiological functions of cells, including tissue homeostasis, cytoskeletal alterations, and intracellular transport. As a major component, microtubules respond to mechanical stimulation by altering their alignment polymerization dynamics. Previously, we reported that may modulate cargo transport one microtubule-associated motor proteins, dynein, under compressive stress. Despite critical role tensile in many biological functions, how on regulates...
Cytoskeletal organization is essential for the precise morphogenesis of cells, tissues, and organs. Cytoskeletons, bound to scaffolding proteins, regulate apical junction complex (AJC), which composed tight adherens junctions, located at side epithelial cell sheets. Cingulin a junction-associated protein that binds both actin filaments microtubules. However, how cingulin microtubules whether can bind simultaneously are unclear. Here we examined mechanisms behind cingulin's...
By facilitating a water/water phase separation (w/wPS), crowded biopolymers in cells form droplets that contribute to the spatial localization of biological components and their biochemical reactions. However, influence on mechanical processes driven by protein motors has not been well studied. Here, we show w/wPS droplet spontaneously entraps kinesins as microtubules (MTs) generates micrometre-scale vortex flow inside droplet. Active with size 10-100 µm are generated through dextran...
Mechanical stress on cells has profound influences biological processes, such as cell shape regulation, the formation of tissue patterns, and development. Recently, mechanosensing properties microtubule, an important cytoskeletal component, have drawn attention. In this work, we studied cargo transport by dynein, a microtubule-associated motor protein, along microtubules deformed under mechanical stress. We reveal that microtubule deformation took place response to applied facilitated...
Cytoplasmic dynein is responsible for various cellular processes during the cell cycle. The mechanism by which its activity regulated spatially and temporarily inside remains elusive. There are regulatory proteins of dynein, including dynactin, NDEL1/NUD-2, LIS1. Characterizing spatiotemporal localization in vivo will aid understanding regulation dynein. Here, we focused on spindle formation Caenorhabditis elegans early embryo, wherein translocated from cytoplasm to region upon nuclear...
Cytoplasmic dynein is a minus-end-directed microtubule-based motor involving various cellular functions including intracellular transport, cell division, and neuronal migration. Recent studies have revealed that single molecules of cytoplasmic do not display unidirectional movement as observed in but diffuse along microtubules. This diffusive behavior transitioned to the via regulatory protein mediated mechanism or self-regulated mechanism. Here, we review recent advances study regulation.