A5065125586- Microtubule and mitosis dynamics
- Cellular transport and secretion
- Micro and Nano Robotics
- Cardiomyopathy and Myosin Studies
- Advanced biosensing and bioanalysis techniques
- Cellular Mechanics and Interactions
- DNA and Nucleic Acid Chemistry
- Molecular Junctions and Nanostructures
- Force Microscopy Techniques and Applications
- Protist diversity and phylogeny
- Bacteriophages and microbial interactions
- Muscle Physiology and Disorders
- Photosynthetic Processes and Mechanisms
- Genomics and Chromatin Dynamics
- Genetic and Kidney Cyst Diseases
- Protein Structure and Dynamics
- Genetic Neurodegenerative Diseases
- Epigenetics and DNA Methylation
- Photoreceptor and optogenetics research
- 14-3-3 protein interactions
- RNA and protein synthesis mechanisms
- Microfluidic and Bio-sensing Technologies
- DNA and Biological Computing
- Diatoms and Algae Research
- Nanofabrication and Lithography Techniques
National Institute of Information and Communications Technology
2016-2025
Advanced Telecommunications Research Institute International
2023
The University of Tokyo
2003-2012
Tokyo University of the Arts
2006-2009
Seiko Holdings (Japan)
2002
Intracellular transport is thought to be achieved by teams of motor proteins bound a cargo. However, the coordination within team remains poorly understood as result experimental difficulty in controlling number and composition motors. Here, we developed an system that links together defined numbers motors with spacing on DNA scaffold. By using this system, linked multiple molecules two different types kinesin motors, processive kinesin-1 or nonprocessive Ncd (kinesin-14), vitro. Both...
Intracellular transport is the basis of microscale logistics within cells and powered by biomolecular motors. Mimicking for in vitro applications has been widely studied; however, inflexibility track design control hindered practical applications. Here, we developed protein-based motors that move on DNA nanotubes combining a motor dynein binding proteins. The new DNA-based nanoarchitectures enabled us to arrange sites track, locally direction movement, achieve multiplexed cargo different...
Abstract The atomic force microscope (AFM), which was invented by Binnig et al. in 1986, can image at nanometer resolution individual biological macromolecules on a substrate solution. This unique capability awoke an expectation of imaging processes occurring work. However, this not met, because the rate with available AFMs too low to capture processes. has last been realized high‐speed AFM developed our research group Kanazawa University. In article, after brief review development...
The motility of biological molecular motors has typically been analyzed by in vitro reconstitution systems using isolated and purified from organs or expressed cultured cells. behavior biomolecular within cells frequently reported to be inconsistent with that observed reconstituted vitro. Although this discrepancy attributed differences ionic strength intracellular crowding, understanding how such parameters affect the remains challenging. In report, we investigated impact crowding on...
LIS1 and NDEL1 are known to be essential for the activity of cytoplasmic dynein in living cells. We previously reported that directly regulated motility an vitro assay. suppressed inhibited translocation microtubules (MTs), while dissociated from MTs restored following suppression by LIS1. However, molecular mechanisms detailed interactions dynein, LIS1, remain unknown. In this study, we dissected regulatory effects on using full-length or truncated recombinant fragments NDEL1. The...
The active transport of proteins and organelles is critical for cellular organization function in eukaryotic cells. A substantial portion long-distance depends on the opposite polarity kinesin dynein family molecular motors to move cargo along microtubules. It increasingly clear that many molecules are moved bi-directionally by both sets motors; however, regulatory mechanism determines directionality remains unclear. We previously reported collapsin response mediator protein-2 (CRMP-2)...
Human mutations in KATNB1 (p80) cause severe congenital cortical malformations, which encompass the clinical features of both microcephaly and lissencephaly. Although p80 plays critical roles during brain development, underlying mechanisms remain predominately unknown. Here, we demonstrate that regulates microtubule (MT) remodeling combination with NuMA (nuclear mitotic apparatus protein) cytoplasmic dynein. We show shuttles between nucleus spindle pole synchrony cell cycle. Interestingly,...
Emergence and collapse of coherent motions self-propelled particles are affected more by particle interactions than their material or biological details. In the reconstructed systems biofilaments molecular motors, several types collective motion including a global-order pattern emerge due to alignment interaction. Meanwhile, earlier studies show that interaction binary collision is too weak form global order. The multiple revealed be important achieve order, but it still unclear what kind...
Fission yeast Pkl1 is a kinesin-14A family member that known to be localized at the cellular spindle and capable of hydrolyzing ATP. However, its motility has not been detected. Here, we show slow, minus end-directed microtubule motor with maximum velocity 33 ± 9 nm/s. The Km,MT value steady-state ATPase activity was as low 6.4 1.1 nm, which 20–30 times smaller than kinesin-1 another member, Ncd, indicating high affinity for microtubules. duty ratio 0.05 indicates spends only small fraction...
Abstract Bipolar mitotic spindles play a critical part in accurate chromosome segregation. During late mitosis, spindle microtubules undergo drastic elongation process called anaphase B. Two kinesin motors, Kinesin-5 and Kinesin-6, are thought to generate outward forces drive elongation, the microtubule crosslinker Ase1/PRC1 maintains structural integrity of antiparallel microtubules. However, how these three proteins orchestrate this remains unknown. Here we explore functional interplay...
Summary Inside cells, molecular motors transport cargoes within the actively fluctuating environment known as cytoplasm. How fluctuations in cytoplasm affect motor-driven is not fully understood. In this study, we investigated role of for along microtubules using C. elegans early embryos, focusing on driven by cytoplasmic dynein. An artificial motor-cargo complex showed faster vivo than vitro , suggesting an acceleration mechanism. We also found that endogenous endosome dynein significantly...
Nanopatterning of motors reveals that the number and spacing kinesin-14, not kinesin-1, dictate microtubule velocity.
Multiciliated cells (MCCs) in tracheas generate mucociliary clearance through coordinated ciliary beating. Apical microtubules (MTs) play a crucial role this process by organizing the planar cell polarity (PCP)–dependent orientation of basal bodies (BBs), for which underlying molecular basis remains elusive. Herein, we found that deficiency Daple, dishevelled-associating protein, tracheal MCCs impaired polarized apical MTs without affecting core PCP proteins, causing significant defects BB...
Myosin 5c (Myo5c) is a low duty ratio, non-processive motor unable to move continuously along actin filaments though it believed participate in secretory vesicle trafficking vertebrate cells. Here, we measured the ATPase kinetics of Myo5c dimers and tested possibility that coupling two molecules enables processive movement. Steady-state activity ADP dissociation demonstrated dimer Myo5c-HMM (double-headed heavy meromyosin 5c) has 6-fold lower Km for than Myo5c-S1 (single-headed myosin...
Cytoplasmic dynein and kinesin are two-headed microtubule motor proteins that move in opposite directions on microtubules. It is known steps by a ‘hand-over-hand’ mechanism, but it unclear which mechanism steps. Because has completely different structure from of its head massive, suspected uses multiple protofilaments microtubules for walking. One way to test this ask whether can step along single protofilament. Here, we examined motility zinc-induced tubulin sheets (zinc-sheets) have only...
Nano-patterning of kinesin molecules to control the number and arrangement motors that transport a single microtubule filament is developed.
Natural kinesin motors are tethered to their cargoes via short C-terminal or N-terminal linkers, whose docking against the core motor domain generates directional force. It remains unclear whether linker is only process contributing force coupled and amplifies an underlying, more fundamental force-generating mechanical cycle of domain. Here, we show that domains double-stranded DNAs (dsDNAs) attached surface loops drive robust microtubule (MT) gliding. Tethering using dsDNA disconnects...