A5101770358- Semiconductor materials and devices
- Semiconductor Quantum Structures and Devices
- Neuroscience and Neuropharmacology Research
- Neuropeptides and Animal Physiology
- Microtubule and mitosis dynamics
- Receptor Mechanisms and Signaling
- Micro and Nano Robotics
- Neural dynamics and brain function
- Advanced Chemical Physics Studies
- GaN-based semiconductor devices and materials
- Pain Mechanisms and Treatments
- Neuroscience and Neural Engineering
- Mitochondrial Function and Pathology
- Metabolism, Diabetes, and Cancer
- Electron and X-Ray Spectroscopy Techniques
- Semiconductor Lasers and Optical Devices
- Amino Acid Enzymes and Metabolism
- Photosynthetic Processes and Mechanisms
- Cellular Mechanics and Interactions
- Advanced Electron Microscopy Techniques and Applications
- Glycosylation and Glycoproteins Research
- Inflammatory mediators and NSAID effects
- Ion channel regulation and function
- Cell Image Analysis Techniques
- Advanced Materials and Mechanics
Kyoto University
2006-2025
RIKEN
1980-2024
Pioneer (Japan)
2024
Kyoto Katsura Hospital
2019-2020
Kwansei Gakuin University
2000-2018
JEOL (Japan)
2017
Toppan (Japan)
2011
Japan Science and Technology Agency
2000-2007
Kyoto Institute of Technology
2005
Max Planck Institute for Solid State Research
1995-2000
Abstract Background We have previously reported that the expression of messenger ribonucleic acid (mRNA) for NR2A subunit N-methyl-D-aspartate (NMDA) class glutamate receptor was decreased in a subset inhibitory interneurons cerebral cortex schizophrenia. In this study, we sought to determine whether deficit mRNA present contain calcium buffer parvalbumin (PV) and associated with reduction glutamatergic inputs prefrontal (PFC) Methods examined mRNA, labeled 35 S-tagged riboprobe, neurons...
Monoclonal antibodies were produced against phosphate-activated glutaminase (EC 3.5.1.2) as a marker for glutamatergic neurons: The enzyme was purified 1000-fold from rat brain mitochondria with recovery of 27%. Upon SDS-PAGE the showed single band up to 1.7 micrograms after silver staining at molecular weight 62,000. Two monoclonal (IgMs) produced; these absorbed more than 90% activity in homogenate. In immunoblotting PAGE homogenate, recognized only 1 protein same position that enzyme....
Eight strains each of Bacillus subtilis, pumilus, licheniformis, and amyloliquefaciens were analyzed by using pyrolysis gas-liquid chromatography. Statistical analysis with canonical variates gave four well-separated groups, which represented the species. Further same deoxyribonucleic acid-deoxyribonucleic acid hybridization API identification systems confirmed discrimination obtained gasliquid However, biochemical tests performed in classical way only three groups since it was not possible...
1. The spatiotemporal pattern of inhibition in the cat motor cortex was studied vitro slice preparations presence 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and 2-amino-5-phosphonovaleric acid (APV). 2. After intracortical microstimulation (0.5-6 microA), fast slow inhibitory postsynaptic potentials (IPSPs) were produced layers II-VI pyramidal cells selectively reduced with bicuculline methiodide phaclofen, respectively. 3. Fast IPSPs maximally by stimulation same layer where their cell...
Antibodies against the mitochondrial enzyme glutaminase (EC 3.5.1.2), have been used in previous immunocytochemical studies to help identify glutamate-releasing neurons among all glutamate-containing neurons. The were based on idea that is enriched within releasable "transmitter" pools of glutamate. However, evidence also available suggest does not occur exclusively Thus we sought determine whether was immunocytochemically detectable presynaptic terminals forming asymmetric (putatively...
The deoxyribonucleic acid homologies among 33 strains of coryneform bacteria with various cell wall types reflected the previous grouping these organisms by Yamada and Komagata on bases mode division, principal amino acids in wall, base composition acid. In addition, following four homology clusters were found: (i) six corynebacteria a glycolyl type (ii) glutamic acid-producing bacteria, (iii) two Brevibacterium linens, (iv) three containing LL-diaminopimelic wall.
The molecular system regulating cellular mechanical properties remains unexplored at single-cell resolution mainly due to a limited ability combine mechanophenotyping with unbiased transcriptional screening. Here, we describe an electroporation-based lipid-bilayer assay for cell surface tension and transcriptomics (ELASTomics), method in which oligonucleotide-labelled macromolecules are imported into cells via nanopore electroporation assess the state of enumerated by sequencing. ELASTomics...
Nine strains of Pseudomonas maltophilia which do not require methionine are described. Methionine-requiring and -nonrequiring P. belong to different biovars, designated I II, respectively; they differ from each other with respect phenotypic characteristics, cellular fatty acid composition, deoxyribonucleic base acid-deoxyribonucleic hybridization. The relationship Xan-thomonas species is also discussed.
Abstract The seasonal pattern of breeding in sheep offers an opportunity to examine plasticity neuronal inputs gonadotropin‐releasing hormone (GnRH) neurones. We used conventional fluorescence microscopy and confocal compare the extent input GnRH neurones from various neuropeptide/neurotransmitter systems ewes during anestrous seasons. Using double‐labelling immunohistochemistry, we counted close appositions between cells varicosities that were immunoreactive for either glutamic acid...
Four forms of dihydrodipicolinate synthase (DHDPS), which catalyzes the first reaction in lysine-specific biosynthetic pathway higher plants, were purified to homogeneity from a suspension culture wheat (Triticum aestivum). These polypeptides have similar N-terminal amino acid sequences. Two different cDNA clones isolated by screening library with oligonucleotide probes based on these The predicted sequences indicate that both encode putative chloroplast transit peptides little homology each...
Pyramidal neurons in layer VI of the primary motor and somatosensory cortices were examined by a combined method intracellular recording, biocytin injection, immunocytochemistry using vitro slice preparations rat brain immunofluorescence staining revealed that biocytin-injected pyramidal cells separated into glutaminase (PAG)-immunopositive PAG-immunonegative cells. Although two groups showed no statistically significant differences passive membrane properties spike characteristics, clear...