A5045988991- Microtubule and mitosis dynamics
- Genetics, Aging, and Longevity in Model Organisms
- Cellular Mechanics and Interactions
- Genomics and Chromatin Dynamics
- Photosynthetic Processes and Mechanisms
- Reproductive Biology and Fertility
- RNA Research and Splicing
- Gene Regulatory Network Analysis
- Nuclear Structure and Function
- Micro and Nano Robotics
- Spaceflight effects on biology
- DNA Repair Mechanisms
- Plant Molecular Biology Research
- Developmental Biology and Gene Regulation
- Chromosomal and Genetic Variations
- Circadian rhythm and melatonin
- Cancer-related gene regulation
- Bioinformatics and Genomic Networks
- Genetics, Bioinformatics, and Biomedical Research
- CRISPR and Genetic Engineering
- Histone Deacetylase Inhibitors Research
- RNA Interference and Gene Delivery
- Photoreceptor and optogenetics research
- Epigenetics and DNA Methylation
- Diatoms and Algae Research
The Graduate University for Advanced Studies, SOKENDAI
2016-2025
National Institute of Genetics
2016-2025
Research Organization of Information and Systems
2012-2024
Marine Biological Laboratory
2015-2024
East Asia School of Theology
2022
Nippon Soken (Japan)
2009-2021
Institute of Pathology Celle
2015-2016
Biologie cellulaire et Cancer
2016
University of Wisconsin–Madison
2010
Osaka University
2009
AbstractFirst paragraph (this article has no abstract) For well over 100 years, cell biologists have been wondering what determines the size of cells. In modern times, we know all molecules that control cycle and division, but still do not understand how is determined. To check whether biology made any inroads on this age-old question, BMC Biology asked several heavyweights in field to tell us they think controlled, drawing a range different types. The essays collection address two related...
Tip60, an HIV-1-Tat interactive protein, is a nuclear histone acetyltransferase (HAT) with unique substrate specificity. Since the acetylation of core histones at particular lysines mediates distinct effects on chromatin assembly and gene regulation, identification lysine site specificity HAT activity Tip60 initial step in analysis its molecular function.Tip60 significantly acetylates amino-terminal tail peptides H2A, H3 H4, but not H2B, consistent preference intact histones. Preferred sites...
The centrosome is generally maintained at the center of cell. In animal cells, centration powered by pulling force microtubules, which dependent on cytoplasmic dynein. However, it unclear how dynein brings to cell center, i.e., structure inside functions as a substrate anchor Here, we provide evidence that population dynein, located intracellular organelles and responsible for organelle transport toward centrosome, generates required in Caenorhabditis elegans embryos. By using database...
Abstract Despite theoretical and physical studies implying that cell-extracellular matrix adhesion geometry governs the orientation of cell division axis, molecular mechanisms translate interphase to mitotic spindle remain elusive. Here, we show cellular edge retraction during rounding correlates with axis. At onset rounding, caveolin-1 is targeted retracting cortical region at proximal end fibres, where ganglioside GM1-enriched membrane domains clusters caveola-like structures are formed in...
Although mechanisms that contribute to microtubule (MT) aster positioning have been extensively studied, still little is known on how asters move inside cells faithfully target a cellular location. Here, we study sperm centration in sea urchin eggs, as stereotypical large-scale movement with extreme constraints centering speed and precision. By tracking three-dimensional dynamics eggs manipulated shapes, show geometry resulting from MT growth interaction cell boundaries dictates...
Cytoplasmic streaming is driven by molecular motors that move along the cytoskeleton and entrain surrounding fluid. In certain cell types, direction of cytoplasmic not predefined but rather stochastic. For example, in meiotic zygotes, swirl reverses over time [Kimura , ]. While a mean-field theory explained reversal, structure flows three-dimensional space mechanical role endoplasmic reticulum (ER) remains unknown. To test hypothesis elastic ER bonds hydrodynamic interactions between...
Background The coactivator p300 acts as a transcriptional adaptor for many DNA‐binding activators. finding that possesses intrinsic acetyltransferase activity which, by chemically modifying histone tails affects the nucleosomal environment and transcription, has greatly advanced our understanding of its function. Subsequent recent studies have shown non‐histone proteins are also acetylated. However, one central question which remained unanswered is how coactivator/acetyltransferase interacts...
Cytoplasmic streaming is a type of intracellular transport widely seen in nature. Caenorhabditis elegans at the one-cell stage bidirectional; flow near cortex (“cortical flow”) oriented toward anterior, whereas central region (“cytoplasmic posterior. Both cortical and cytoplasmic depend on non-muscle-myosin II (NMY-2), which primarily localizes cortex. The manner NMY-2 proteins drive opposite direction from remote locations has not been fully understood. In this study, we demonstrated that...
Eukaryotic gene expression is regulated in the context of chromatin. Dynamic changes post-translational histone modification are thought to play key roles fundamental cellular functions such as regulation cell cycle, development, and differentiation. To elucidate relationship between modifications functions, it important monitor dynamics single living cells. A genetically encoded probe called mintbody (modification-specific intracellular antibody), which a single-chain variable fragment...
Here we show a novel pathway of transcriptional regulation DNA-binding transcription factor by coupled interaction and modification (e.g., acetylation) through the domain (DBD). The oncogenic regulator SET was isolated affinity purification factors interacting with DBD cardiovascular KLF5. negatively regulated KLF5 DNA binding, transactivation, cell-proliferative activities. Down-regulation negative seen in response to KLF5-mediated gene activation. coactivator/acetylase p300, on other hand,...
During open mitosis in higher eukaryotic cells, the nuclear envelope completely breaks down and then mitotic chromosomes are exposed cytoplasm. By contrast, lower eukaryotes, including fungi, proceeds with nucleus enclosed an intact envelope. The mechanism of has been studied extensively yeast, a closed organism. Here, we describe form which is torn by elongation fission yeast Schizosaccharomyces japonicus. Sz. japonicus adopted fusiform shape anaphase, its following extension caused...
Notch signaling exerts multiple roles during different steps of mouse somitogenesis. We have previously shown that segmental boundaries are formed at the interface activity boundary, suggesting importance on/off state for boundary formation. However, a recent study has embryos expressing Notch-intracellular domain (NICD) throughout presomitic mesoderm (PSM) can still form more than ten somites, indicating NICD is dispensable To clarify this discrepancy in our current study, we created...
The number of centrioles is tightly controlled to ensure bipolar spindle assembly, which a prerequisite maintain genome integrity. However, our understanding the fundamental principle that governs formation single procentriole per parental centriole incomplete. Here, we show local restriction Plk4, master regulator formation, achieved by bimodal interaction STIL with Plk4. We demonstrate conserved short coiled-coil region binds and protects Plk4 from protein degradation at site formation. On...
Centrosome positioning is actively regulated by forces acting on microtubules radiating from the centrosomes. Two mechanisms, center-directed and polarized cortical pulling, are major contributors to successive centering posteriorly displacing migrations of centrosomes in single-cell-stage Caenorhabditis elegans. In this study, we analyze spatial distribution examine mechanism that switches centrosomal migration displacing. We clarify using image processing measure micrometer-scale movements...
The mitotic spindle is a diamond-shaped molecular apparatus crucial for chromosomal segregation. regulation of length well studied, but little known about width. Previous studies suggested that the can self-organize to maintain constant aspect ratio between its and width against physical perturbations. Here we determine widths metaphase spindles various sizes observed during embryogenesis in Caenorhabditis elegans, including small obtained by knocking down tpxl-1 or spd-2 gene. correlates...
Chromosome condensation is critical for accurate inheritance of genetic information. The degree condensation, which reflected in the size condensed chromosomes during mitosis, not constant. It differentially regulated embryonic and somatic cells. In addition to developmentally programmed regulation chromosome there may be adaptive based on spatial parameters such as genomic length or cell size. We propose that affected by a parameter called amount per nuclear space, “intranuclear DNA...
During early embryogenesis in animals, cells are arranged into a species-specific pattern robust manner. Diverse cell arrangement patterns observed, even among close relatives. In the present study, we evaluated mechanisms by which diversity and robustness of arrangements achieved developing embryos. We successfully reproduced various observed nematode species Caenorhabditis elegans embryos altering eggshell shapes. The findings suggest that can be explained differences shape. Additionally,...