A5058121264- Genetic and Kidney Cyst Diseases
- Microtubule and mitosis dynamics
- Microfluidic and Capillary Electrophoresis Applications
- Protist diversity and phylogeny
- Micro and Nano Robotics
- Congenital heart defects research
- Flow Measurement and Analysis
- Microfluidic and Bio-sensing Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Renal and related cancers
- Fullerene Chemistry and Applications
- Heat shock proteins research
- Analytical Chemistry and Sensors
- Enzyme Structure and Function
- Electrowetting and Microfluidic Technologies
- Graphene research and applications
- Diamond and Carbon-based Materials Research
- Tissue Engineering and Regenerative Medicine
- Fluid Dynamics and Mixing
- Genetic Syndromes and Imprinting
- Fluid Dynamics and Turbulent Flows
- Wnt/β-catenin signaling in development and cancer
- Particle Dynamics in Fluid Flows
- Machine Learning in Materials Science
- Electrohydrodynamics and Fluid Dynamics
Tokyo University of Agriculture and Technology
2017-2024
Osaka University
2009-2017
Japan Science and Technology Agency
2012-2013
Ube Frontier University
2006-2012
The University of Tokyo
2003-2008
Quest University Canada
2005
Tokyo University of Science
2004
Tokai University
2003
Distinguishing Right from Left In most vertebrates during embryonic development, rotational movement of the cilia within a structure in embryo, known as node, generates unidirectional flow required for future left-right asymmetry internal organs. The may transport determinant molecule or provide mechanical force. However, it is not clear how sensed. Yoshiba et al. (p. 226 , published online 13 September; see Perspective by Norris and Grimes ) show that nodal mouse embryos sensed perinodal...
Cytoplasmic streaming is a type of intracellular transport widely seen in nature. Caenorhabditis elegans at the one-cell stage bidirectional; flow near cortex (“cortical flow”) oriented toward anterior, whereas central region (“cytoplasmic posterior. Both cortical and cytoplasmic depend on non-muscle-myosin II (NMY-2), which primarily localizes cortex. The manner NMY-2 proteins drive opposite direction from remote locations has not been fully understood. In this study, we demonstrated that...
During mammalian development, left-right (L-R) asymmetry is established by a cilia-driven leftward fluid flow within midline embryonic cavity called the node. This 'nodal flow' detected peripherally-located crown cells that each assemble primary cilium which contain putative Ca2+ channel PKD2. The interaction of and cell cilia promotes left side-specific expression Nodal in lateral plate mesoderm (LPM). Whilst PKD2-interacting protein PKD1L1 has also been implicated L-R patterning,...
This paper reports a new technique of micro-resolution particle image velocimetry (PIV). To investigate transient phenomena in microfluidic device, high-speed micro-PIV was developed by combining camera and continuous wave (CW) laser. The applied to micro counter-current flow, consisting water butyl acetate. velocity fields the flow were visualized for time resolution 500 µs spatial 2.2 × µm2. Using technique, vortex-like motions fluorescent particles around water–butyl acetate interface...
Axonemal dynein complexes are preassembled in the cytoplasm before their transport to cilia, but mechanism of this process remains unclear. We now show that mice lacking Pih1d3, a PIH1 domain–containing protein, develop normally manifest male sterility. Pih1d3−/− sperm were immotile and fragile, with axoneme flagellum outer arms (ODAs) inner (IDAs) showing disturbed 9+2 microtubule organization. Pih1d3 was expressed specifically spermatogenic cells, mRNA being most abundant pachytene...
Lrrc6 encodes a cytoplasmic protein that is expressed specifically in cells with motile cilia including the node, trachea and testes of mice. A mutation has been identified human patients primary ciliary dyskinesia ( PCD ). Mutant mice lacking show typical defects such as hydrocephalus laterality defects. We found absence Lrrc6, morphology remained normal, but their motility was completely lost. The 9 + 2 arrangement microtubules normal ‐/‐ mice, outer dynein arms ODA s), structures...
Motile cilia/flagella are essential for swimming and generating extracellular fluid flow in eukaryotes. cilia harbor a 9+2 arrangement consisting of nine doublet microtubules with dynein arms at the periphery pair singlet center (central pair). In central system, radial spoke has T-shaped architecture regulates motility motion pattern cilia. Recent cryoelectron tomography data reveal three types spokes (RS1, RS2, RS3) 96 nm axoneme repeat unit; however, molecular composition third spoke, RS3...
The determination of left-right body asymmetry in mouse embryos depends on the interplay molecules a highly sensitive structure, node. Here, we show that localization Cerl2 protein does not correlate to its mRNA expression pattern, from 3-somite stage onwards. Instead, displays nodal flow-dependent dynamic behavior controls activity Nodal node, and transmission laterality information left lateral plate mesoderm (LPM). Our results indicate initially localizes prevents activation genetic...
Cellular structures are hydrodynamically interconnected, such that force generation in one location can move distal structures. One example of this phenomenon is cytoplasmic streaming, whereby active forces at the cell cortex induce streaming entire cytoplasm. However, it not known how spatial distribution and magnitude these distant objects within cell. To address issue, we developed a computational method used cytoplasm hydrodynamics to infer shear stress induced by generators from...
The interaction between chemical reactions and the flow field in microfluidic devices is investigated by a laser-induced fluorescence technique refined for use at microscopic spatial resolution. pH distribution of chemically reacting Y-junction neutralization reaction device successfully visualized resolution 0.89 µm × µm.
Left-right (L-R) asymmetry in the body plan is determined by nodal flow vertebrate embryos. Shinohara et al. (Shinohara K 2012 Nat. Commun.3, 622 (doi:10.1038/ncomms1624)) used Dpcd and Rfx3 mutant mouse embryos showed that only a few cilia were sufficient to achieve L-R asymmetry. However, mechanism underlying breaking of symmetry such weak ciliary unclear. Flow-mediated signals associated with asymmetric organogenesis have not been clarified, two different hypotheses-vesicle transport...
Rotational movement of mouse node cilia generates leftward fluid flow in the cavity, playing an important role left-right determination embryo. Although rotation numerous was believed necessary to trigger determination, recent reports indicate action two be sufficient. We examine cooperative via hydrodynamic interaction. Results show cooperative, having phases locked a certain relation; system with pair nonidentical can achieve phase-locked states more easily than one identical cilia.
We have carried out screening of C60 crystallization using a simple liquid/liquid interfacial precipitation method in microfluidic device. By controlling the time, temperature, and concentration, various metastable phases crystals were found, including tubes, spheres, open-ended hollow columns, stars, branches, trees. The obtained crystal shapes are similar to those snow crystals. These findings suggest an urgent need screen for development fullerene drugs.
The measurement of a three-component (3C) velocity field in microfluidic devices with conventional techniques and micro-PIV (particle image velocimetry) is still difficult due to limited optical access. Since Santiago et al (1998 Exp. Fluids 25 316–9), flow measurements have remained mainly 2C vector realizations. In this study, the third component velocity, i.e. out-of-plane extraction from two-dimensional time-resolved (TR) images, proposed. method based on PIV performs cross-correlation...