A5079886500- Gene Regulatory Network Analysis
- Insect-Plant Interactions and Control
- Plant and animal studies
- Fossil Insects in Amber
- Insect behavior and control techniques
- Insect Pest Control Strategies
- Microbial Metabolic Engineering and Bioproduction
- Hymenoptera taxonomy and phylogeny
- Insect Resistance and Genetics
- Bioinformatics and Genomic Networks
- Studies on Chitinases and Chitosanases
- Cellular Mechanics and Interactions
- Enzyme Production and Characterization
- Aquaculture Nutrition and Growth
- Insect and Pesticide Research
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Biological Control of Invasive Species
- Aquaculture disease management and microbiota
- Evolution and Genetic Dynamics
- Remote Sensing in Agriculture
- Protein Hydrolysis and Bioactive Peptides
- Forest Insect Ecology and Management
- Protein Structure and Dynamics
- Animal Genetics and Reproduction
Kyoto University
2018-2025
Chiba Prefectural Agriculture and Forestry Research Center Forest Research Institute
2016-2023
RIKEN Advanced Science Institute
2011-2021
RIKEN
2009-2021
National Institute for Agro-Environmental Sciences
2012-2021
University of California, Berkeley
2021
Tokyo Institute of Technology
2010-2020
University of Toyama
2020
National Agriculture and Food Research Organization
2019
Japan Science and Technology Agency
2009-2018
A series of chimeric promoters for higher-level expression foreign genes in plants was constructed as fusions a gene rβ-glucuronidase (GUS) with the terminator nopaline synthase (nos) or cauliflower mosaic virus (CaMV) 35S transcript, and strength these assayed transient stable systems tobacco rice. As parts promoters, CaMV core promoter, three different 5′-upstream sequences first intron phaseolin, 5′-untranslated sequence (Ω sequence) were used various combinations. In rice protoplasts,...
Various molecules exclusively accumulate at the front or back of migrating eukaryotic cells in response to a shallow gradient extracellular signals. Directional sensing and signal amplification highlight essential properties cells, known as cell polarity. In addition these, such polarity involve unique determination direction (uniqueness axis) localized edge (localization sensitivity), both which may be required for smooth migration. Here we provide mass conservation system based on...
Modern biology provides many networks describing regulations between species of molecules. It is widely believed that the dynamics molecular activities based on such regulatory are origin biological functions. However, we currently have a limited understanding relationship structure network and its dynamics. In this study develop new theory to provide an important aspect from information linkages alone. We show "feedback vertex set" (FVS) set "determining nodes" The powerful real systems in...
During gastrulation in the mouse embryo, dynamic cell movements including epiblast invagination and mesodermal layer expansion lead to establishment of three-layered body plan. The precise details these movements, however, are sometimes elusive, because limitations live imaging. To overcome this problem, we developed techniques enable observation living embryos with digital scanned light sheet microscope (DSLM). achieved deep high time-resolution images GFP-expressing nuclei following 3D...
Jasmonic acid (JA) plays an important role in the induction of herbivore resistance many plants. However, JA-independent has been suggested. An herbivore-resistance-inducing substance was isolated from Tobacco mosaic virus-infected tobacco (Nicotiana tabacum) leaves which a hypersensitive response (HR) induced and identified as loliolide, β-carotene metabolite. When applied to tomato (Solanum lycopersicum) leaves, loliolide decreased survival rate two-spotted spider mite, Tetranychus...
Abstract Rho GTPases play crucial roles in cell polarity and pattern formation. ROPs, of plant GTPases, are widely involved wall patterning plants, yet the molecular mechanism underlying their action remains unknown. Arabidopsis ROP11 is locally activated to form plasma membrane domains, which direct formation pits metaxylem vessel cells through interaction with cortical microtubules. Here, we show that governed by ROP activation via a reaction-diffusion mechanism. Genetic analysis...
Neurons develop dendritic arbors in cell type-specific patterns. Using growing Purkinje cells culture as a model, we performed long-term time-lapse observation of dendrite branch dynamics to understand the rules that govern characteristic space-filling dendrites. We found architecture was sculpted by combination reproducible dynamic processes, including constant tip elongation, stochastic terminal branching, and retraction triggered contacts between Inhibition protein kinase C/protein D...
Dendritic trees influence synaptic integration and neuronal excitability, yet appear to develop in rather arbitrary patterns. Using electron microscopy serial reconstructions, we analyzed the dendritic of four morphologically distinct neocortical interneuron subtypes reveal two underlying organizational principles common all. First, cross-sectional areas at any given point within a dendrite were proportional summed length all segments distal that point. Consistent with this observation,...
Significance To date, there is no experimental method for examination of detailed morphological dynamics a cellular organelle, because the small size. The Golgi apparatus, membrane-bounded decomposes into fragments during cell division, and reassemble to form characteristic shape in daughter cells, which also unobservable. understand reassembly process, we developed three-dimensional simulator based on coarse-grained physical membrane model. Our simulation reproduced fine revealed...
Abstract Pyrophosphate (PPi) is produced by anabolic reactions and serves as an energy donor in the cytosol of plant cells; however, its accumulation to toxic levels disrupts several common biosynthetic pathways lethal. Before acquiring photosynthetic capacity, young seedlings must endure a short but critical heterotrophic period, during which they are nourished solely sugar from seed reserves process gluconeogenesis. Previously, we reported that excess PPi H + -PPase-knockout fugu5 mutants...
Abstract In some mammalian genes, the paternally and maternally derived alleles are expressed differently: this phenomenon is called genomic imprinting. Here we study evolution of imprinting using multivariate quantitative genetic models to examine feasibility conflict hypothesis. This hypothesis explains observed patterns as an evolutionary outcome between paternal maternal alleles. We consider expression a zygotic gene, which codes for embryonic growth factor affecting amount resources...
In living cells, chemical reactions are connected by sharing their products and substrates, form complex networks, e.g. metabolic pathways. Here we developed a theory to predict the sensitivity, i.e. responses of concentrations fluxes perturbations enzymes, from network structure alone. Responses turn out exhibit two characteristic patterns, $localization$ $hierarchy$. We present general theorem connecting sensitivity with topology that explains these patterns. Our results imply is an origin...
Physiological functions of cells arise from the dynamics chemical reaction networks. The cell cycle fission yeast is controlled by dynamical changes in two cyclin-dependent kinase (CDK)-cyclin complexes based on a complicated network consisting protein synthesis, complex formation, and degradation. Each checkpoints, G1-S G2-M, driven an increase concentration CDK-Cig2 CDK-Cdc13, respectively. However, it not understood how these single connected are independently stage-specific manner. Here...