A5044706706- Congenital heart defects research
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Cardiac Fibrosis and Remodeling
- Developmental Biology and Gene Regulation
- Electrospun Nanofibers in Biomedical Applications
- Cardiac Ischemia and Reperfusion
- Immune Response and Inflammation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive System and Pregnancy
- Pluripotent Stem Cells Research
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Pancreatic function and diabetes
- Cardiovascular Function and Risk Factors
- MicroRNA in disease regulation
- Genomics and Chromatin Dynamics
- Advanced biosensing and bioanalysis techniques
- Muscle Physiology and Disorders
- Wind Turbine Control Systems
- Helicobacter pylori-related gastroenterology studies
- Retinoids in leukemia and cellular processes
- Cell Image Analysis Techniques
- Microtubule and mitosis dynamics
- Cancer-related gene regulation
Kyoto Prefectural University of Medicine
2019-2025
William Harvey Research Institute
2009-2024
Queen Mary University of London
2009-2024
Osaka University
2006-2020
The University of Queensland
2006-2019
Endo Pharmaceuticals (United States)
2016
Japan Science and Technology Agency
2004-2012
Lung Institute
2008
Imperial College London
2008
Harefield Hospital
2008
Germ cells in the mouse embryo can develop as oocytes or spermatogonia, depending on molecular cues that have not been identified. We found retinoic acid, produced by mesonephroi of both sexes, causes germ ovary to enter meiosis and initiate oogenesis. Meiosis is retarded fetal testis action retinoid-degrading enzyme CYP26B1, ultimately leading spermatogenesis. In testes Cyp26b1-knockout embryos, precociously, if a normal ovary. Thus, precise regulation retinoid levels during gonad...
Alternatively activated (also known as M2) macrophages are involved in the repair of various types organs. However, contribution M2 to cardiac after myocardial infarction (MI) remains be fully characterized. Here, we identified CD206+F4/80+CD11b+ M2-like murine heart and demonstrated that this cell population predominantly increases infarct area exhibits strengthened reparative abilities MI. We evaluated mice lacking kinase TRIB1 (Trib1-/-), which exhibit a selective depletion Compared with...
Toll-like receptors (TLRs) are the central players in innate immunity. In particular, TLR9 initiates inflammatory response by recognizing DNA, imported infection or released from tissue damage. Inflammation is, however, harmful to terminally differentiated organs, such as heart and brain, with poor regenerative capacity, yet role of nonimmune cells, including cardiomyocytes neurons, is undefined. Here we uncover an unexpected energy metabolism cellular protection neurons. stimulation reduced...
Transplantation of bone marrow-derived mesenchymal stromal cells (MSCs) is an emerging treatment for heart failure based on their secretion-mediated "paracrine effects". Feasibility the scaffoldless cell sheet technique to enhance outcome transplantation has been reported using other types, though mechanism underpinning enhancement remains uncertain. We here investigated role this innovative amplify effects MSC with a focus underlying factors. After coronary artery ligation in rats,...
Scientific Report7 March 2014Open Access Source Data Toll-like receptor 9 protects non-immune cells from stress by modulating mitochondrial ATP synthesis through the inhibition of SERCA2 Yasunori Shintani Corresponding Author William Harvey Research Institute, Barts and The London School Medicine Dentistry, Queen Mary University London, UK Department Medical Biochemistry, Osaka Graduate Medicine, Suita, Japan Search for more papers this author Hannes CA Drexler Bioanalytical Mass...
Pitx2 is expressed in developing visceral organs on the left side and implicated left-right (LR) asymmetric organogenesis. The expression of controlled by an intronic enhancer (ASE) that contains multiple Foxh1-binding sites Nkx2-binding site. These binding are essential sufficient for activity evolutionarily conserved among vertebrates. We now show mice lack ASE (Pitx2(Delta)(ASE/)(Delta)(ASE) mice) fail to manifest left-sided exhibit laterality defects most organs, although position...
Background— Inflammation plays an important role in the progress of adverse ventricular remodeling after myocardial infarction. High-mobility group box 1 (HMGB1) is a nuclear protein, which has recently been uncovered to also act as modifier inflammation when released. We hypothesized that HMGB1 injection could preferentially modulate local inflammation, attenuate remodeling, and subsequently improve cardiac performance postinfarction chronic heart failure. Methods Results— Three weeks left...
Highlights•LIMD1 is necessary for the Akt3-dependent assembly of an AGO-TNRC6A functional miRISC•AGO1, 2, and 4 recruit LIMD1 in a phospho-dependent manner•AGO2 to AGO3 switching occurs upon ablation HeLa cells•AGO3-WTIP replaces AGO2-LIMD1 miRNA silencing Akt3-independent mannerSummaryAs core components microRNA-induced complex (miRISC), Argonaute (AGO) proteins interact with TNRC6 proteins, recruiting other effectors translational repression/mRNA destabilization. Here, we show that...
MicroRNA miR-214 has been implicated in many biological cellular functions, but the impact of and its target genes on vascular smooth muscle cell (VSMC) proliferation, migration, neointima hyperplasia is unknown.
Abstract Sonic Hedgehog ( Shh ), encoding an extracellular signaling molecule, is vital for heart development. null mutants show congenital disease due to left–right asymmetry defects stemming from functional anomaly in the midline structure mice. also known affect cardiomyocyte differentiation, endocardium development, and morphogenesis, particularly second field (SHF) cardiac progenitor cells that contribute right ventricle, outflow tract, parts of atrium. Despite extensive studies, our...
The abundance of retinoic acid (RA) is determined by the balance between its synthesis retinaldehyde dehydrogenase (RALDH) and degradation CYP26. In particular, dynamic expression three CYP26 genes controls regional level RA within body. Pregastrulation mouse embryos express but not RALDH. We now show that mice lacking all manifest duplication body axis as a result expansion Nodal domain throughout epiblast. Mouse was found to contain an RA-responsive element in intron 1 highly conserved...
In the early vertebrate embryo, cardiac progenitor/precursor cells (CPs) give rise to structures. Better understanding their biological character is critical understand heart development and apply CPs for clinical arena. However, our knowledge remains incomplete. With use of single-cell expression profiling, we have now revealed rapid dynamic changes in gene profiles embryonic during phase after segregation from mesoderm. Progressively, nascent mesodermal Mesp1 terminated, Nkx2-5+/Tbx5+...
Transplantation of allogeneic mesenchymal stromal cells (MSCs) is a promising treatment for heart failure. We have shown that epicardial placement cell sheets markedly increases donor survival and augments therapeutic effects compared with the current methods. Although immune rejection intramyocardially injected MSCs been suggested, transplanted on surface (virtual space) may undergo different courses. This study aimed to elucidate immunologic response against epicardially placed MSCs, or...
A surface marker that distinctly identifies cardiac progenitors (CPs) is essential for the robust isolation of these cells, circumventing necessity genetic modification. Here, we demonstrate a Glycosylphosphatidylinositol-anchor containing neurotrophic factor receptor, Glial cell line-derived receptor alpha 2 (Gfra2), specifically marks CPs. GFRA2 expression facilitates CPs by fluorescence activated sorting from differentiating mouse and human pluripotent stem cells. Gfra2 mutants reveal an...
Background: Embryonic stem (ES) cells are pluripotent with the ability to differentiate any cell type of resident organism. In recent years, significant advances have been made in using these obtain large numbers cardiomyocyte (CM) ‐like for scientific research and clinical application. A vast number protocols emerged describing differentiation methods without use animal serum or extracts restrictive a human setting. These techniques follow complicated procedure, which although successful,...