A5089672501- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Acute Lymphoblastic Leukemia research
- Pluripotent Stem Cells Research
- Chromosomal and Genetic Variations
- RNA Research and Splicing
- Pancreatic and Hepatic Oncology Research
- DNA Repair Mechanisms
- Cancer-related gene regulation
- Genomics and Phylogenetic Studies
- Cancer Genomics and Diagnostics
- Advanced biosensing and bioanalysis techniques
- Congenital heart defects research
- Wind Turbine Control Systems
- Multilevel Inverters and Converters
- Zebrafish Biomedical Research Applications
- Plant Molecular Biology Research
- Pancreatic function and diabetes
Queen Mary University of London
2012-2015
William Harvey Research Institute
2012-2015
Nordic Lymphoma Group
2013
Imperial College London
2007-2013
Medical Research Council
2010
Hammersmith Hospital
2009
SUNY Upstate Medical University
2005-2006
Mono-, di-, and trimethylation of specific histone residues adds an additional level complexity to the range modifications that may contribute a code. However, it has not been clear whether different methylated states reside stably at chromatin sites or they represent dynamic intermediates same sites. Here, we have used recently developed antibodies are highly for mono-, trimethylated lysine 9 H3 (MeK9H3) examine subnuclear localization replication timing containing these epigenetic marks in...
REST is a transcriptional repressor that targets group of neuronal genes in non-neuronal cells. In embryonic stem (ES) cells, has been implicated controlling the expression transcription factor are crucial for lineage determination and maintaining ES cell potential. Here, we asked whether directly regulates neural-specifying mouse cells using siRNA-mediated knockdown lack functional protein as result gene targeting. Loss did not affect any ten known to promote neural commitment several...
The time of locus replication during S-phase is tightly regulated and correlates with chromatin state. Embryonic stem (ES) cells have an unusual profile where many developmental regulator genes that are not yet expressed marked by both active repressive histone modifications. This poised or bivalent state also characterized in early ES cells, while timing delayed restricted options. Here we used a panel mutant mouse cell lines lacking important modifiers to dissect the relationship between...
In the early vertebrate embryo, cardiac progenitor/precursor cells (CPs) give rise to structures. Better understanding their biological character is critical understand heart development and apply CPs for clinical arena. However, our knowledge remains incomplete. With use of single-cell expression profiling, we have now revealed rapid dynamic changes in gene profiles embryonic during phase after segregation from mesoderm. Progressively, nascent mesodermal Mesp1 terminated, Nkx2-5+/Tbx5+...
Abstract Background : Deep sequencing of single cell‐derived cDNAs offers novel insights into oncogenesis and embryogenesis. However, traditional library preparation for RNA‐seq analysis requires multiple steps with consequent sample loss stochastic variation at each step significantly affecting output. Thus, a simpler better protocol is desirable. The recently developed hyperactive Tn5‐mediated preparation, which brings high quality libraries, likely one the solutions. Results Conclusions...
Histone modifications are central to epigenetic regulation and must be reestablished with each round of DNA replication. Here we describe methods localize these within mammalian nuclei relate them specific spatiotemporal patterns