A5080737786- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Neurological disorders and treatments
- Neuroscience and Neural Engineering
- Memory and Neural Mechanisms
- Receptor Mechanisms and Signaling
- Neurotransmitter Receptor Influence on Behavior
- Genetic Neurodegenerative Diseases
- Neurobiology and Insect Physiology Research
- Visual perception and processing mechanisms
- Functional Brain Connectivity Studies
- Advanced Fluorescence Microscopy Techniques
- Sleep and Wakefulness Research
- Lipid Membrane Structure and Behavior
- Proteoglycans and glycosaminoglycans research
- Photoreceptor and optogenetics research
- Insect and Arachnid Ecology and Behavior
- Neuropeptides and Animal Physiology
- Animal Behavior and Reproduction
- stochastic dynamics and bifurcation
- Salivary Gland Tumors Diagnosis and Treatment
- Antioxidant Activity and Oxidative Stress
- Biotin and Related Studies
- Cardiovascular Function and Risk Factors
- Cell Image Analysis Techniques
Hokkaido University
2021-2024
Doshisha University
2013-2022
University of Debrecen
2007-2016
National Institute for Physiological Sciences
2004-2015
Japan Science and Technology Agency
2010-2015
University of Dundee
2007
Medical Research Council
2007
University of Oxford
2007
Tokyo Medical and Dental University
2007
Tokyo University of Agriculture
1999
Whether neocortical γ-aminobutyric acid (GABA) cells are composed of a limited number distinct classes neuron, or whether they continuously differentiated with much higher diversity, remains contentious issue for the field. Most GABA rat frontal cortex have at least 1 6 chemical markers (parvalbumin, calretinin, alpha-actinin-2, somatostatin, vasoactive intestinal polypeptide, and cholecystokinin), each class comprising several neuronal subtypes having specific physiological morphological...
Although neocortical GABAergic (γ-aminobutyric acidergic) interneurons have been the focus of intense study, especially in rat, a consensus view functional diversity and organization inhibitory cortical neurons has not yet achieved. To better analyze we used bacterial artificial chromosome (BAC) construct established 2 lines transgenic rats that coexpress Venus, yellow fluorescent protein, with vesicular GABA transporter. The brain content from both was similar to level found wild-type rats....
GABAergic nonpyramidal cells, cortical interneurons, consist of heterogeneous subtypes differing in their axonal field and target selectivity. It remains to be investigated how the diverse innervation patterns are generated these spatially complicated, but synaptically specific wirings achieved. Here, we asked whether a particular cell type obeys branching bouton arrangement principle or differs from others only average morphometric values morphological template common cells. For this...
Fast inhibition in the cortex is gated primarily at GABAergic synapses formed by local interneurons onto postsynaptic targets. Although inputs to somata and axon initial segments of neocortical pyramidal neurons are associated with direct action potential generation, role distal dendritic segments, including spines, less well characterized. Because a significant proportion inhibitory input occurs on dendrites it will be important determine whether these selectively certain classes...
Diabetic cardiomyopathy has been reported to increase the risk of fatal ventricular arrhythmia. The beneficial effects selective sodium-glucose cotransporter-2 inhibitor have not fully examined in context antiarrhythmic therapy, especially its direct cardioprotective despite negligible SGLT2 expression cardiomyocytes. We aimed examine empagliflozin (EMPA) treatment on diabetic cardiomyocytes, with a special focus Ca2+ handling. conducted echocardiography and hemodynamic studies studied...
Despite widespread adoption of tissue clearing techniques in recent years, poor access to suitable light-sheet fluorescence microscopes remains a major obstacle for biomedical end-users. Here, we present descSPIM (desktop-equipped SPIM cleared specimens), low-cost ($20,000-50,000), low-expertise (one-day installation by non-expert), yet practical do-it-yourself microscope as solution this bottleneck. Even the most fundamental configuration enables multi-color imaging whole mouse brains and...
To understand the dendritic differentiation in various types of cortical nonpyramidal cells, we analyzed quantitatively their branching and spine expression. The internode interspine interval obeyed exponential distributions with type-specific decay constants. initial pattern, density at light microscopic level divided cells into three types, correlated axonal, neurochemical firing types. pattern determined overall vertical spread dendrites. Basket cell subtypes different chemical expression...
Inhibitory interneurons target precise membrane regions on pyramidal cells, but differences in their functional effects somata, dendrites and spines remain unclear. We analyzed inhibitory synaptic events induced by cortical, fast-spiking (FS) basket cells which innervate dendritic shafts as well cell somata. Serial electron micrograph (EMg) reconstructions showed that somatic synapses were larger than contacts. Simulations with anatomical physiological data reveal between different...
The supramammillary nucleus (SuM) of the hypothalamus projects to dentate gyrus (DG) and CA2 region hippocampus. Although SuM-to-hippocampus circuits have been implicated in spatial emotional memory formation, little is known about precise neural connections between SuM Here, we report that axons neurons make monosynaptic granule cells (GCs) GABAergic interneurons, but not hilar mossy cells, DG co-release glutamate γ-aminobutyric acid (GABA) at these synapses. inputs from can excite some...
The basal ganglia are critical for the control of motor behaviors and reinforcement learning. Here, we demonstrate in rats that primary secondary areas (M1 M2) make functional synaptic connections globus pallidus (GP), not usually thought as an input site ganglia. Morphological observation revealed density axonal boutons from cortices GP was 47% 78% subthalamic nucleus (STN) M1 M2, respectively. Cortical excitation neurons comparable to STN slice preparations. FoxP2-expressing arkypallidal...
How information is manipulated and segregated within local circuits in the frontal cortex remains mysterious, part because of inadequate knowledge regarding connectivity diverse pyramidal cell subtypes. The participates formation retrieval declarative memories through projections to perirhinal cortex, procedural learning striatum/pontine nuclei. In rat we identified two subtypes selectively projecting distinct subregions (PRC). PRC-projecting cells upper layer 2/3 (L2/3) projected area 35,...
Dendritic trees influence synaptic integration and neuronal excitability, yet appear to develop in rather arbitrary patterns. Using electron microscopy serial reconstructions, we analyzed the dendritic of four morphologically distinct neocortical interneuron subtypes reveal two underlying organizational principles common all. First, cross-sectional areas at any given point within a dendrite were proportional summed length all segments distal that point. Consistent with this observation,...
Perineuronal nets (PNNs), composed mainly of chondroitin sulfate proteoglycans, are the extracellular matrix that surrounds cell bodies, proximal dendrites, and axon initial segments adult CNS neurons. PNNs known to regulate neuronal plasticity, although their physiological roles in cerebellar functions have yet be elucidated. Here, we investigated contribution GABAergic transmission from Purkinje cells (PCs) large glutamatergic neurons deep nuclei (DCN) male mice by recording IPSCs slices,...
The brain networks responsible for adaptive behavioral changes are based on the physical connections between neurons. Light and electron microscopy have long been used to study neural projections Volume has recently expanded its scale of analysis due methodological advances, resulting in complete wiring maps neurites a large volume tissues even entire nervous systems growing number species. However, structural approaches frequently suffer from inherent limitations which elements images...
Our aim was to reveal the relationship between layer IV horizontal connections and functional architecture of cat primary visual cortex because these play important roles in first cortical stage signals integration. We investigated bouton distribution spiny neurons over an orientation preference map using vivo optical imaging, unit recordings, single neuron reconstructions. The radial extent reconstructed axons (14 star pyramidal 9 stellate cells) ∼1.5 mm. In vicinity parent somata (<400...
Abstract In rodents, the dorsolateral striatum regulates voluntary movement by integrating excitatory inputs from motor‐related cerebral cortex and thalamus to produce contingent inhibitory output other basal ganglia nuclei. Striatal parvalbumin (PV)‐producing interneurons receiving this input then inhibit medium spiny neurons (MSNs) modify their outputs. To understand function in motor control, it is important reveal precise synaptic organization of cortical thalamic striatal PV...