A5073378373- Periodontal Regeneration and Treatments
- Connective tissue disorders research
- Bone and Dental Protein Studies
- dental development and anomalies
- Bone Metabolism and Diseases
- Bone health and osteoporosis research
- Protease and Inhibitor Mechanisms
- Bone health and treatments
- TGF-β signaling in diseases
- Dental Implant Techniques and Outcomes
- Microbial metabolism and enzyme function
- Bone Tissue Engineering Materials
- Mesenchymal stem cell research
- Oral microbiology and periodontitis research
- Heterotopic Ossification and Related Conditions
- Genetic and Kidney Cyst Diseases
- Endodontics and Root Canal Treatments
- Proteoglycans and glycosaminoglycans research
- Collagen: Extraction and Characterization
- Dental Trauma and Treatments
- Cell Adhesion Molecules Research
- 3D Printing in Biomedical Research
- Osteoarthritis Treatment and Mechanisms
- Dental materials and restorations
- Orthopaedic implants and arthroplasty
Niigata University
2015-2024
Niigata University Medical and Dental Hospital
2012-2020
Nagasaki University
2018
Nagasaki University Hospital
2018
In-Q-Tel
2017
Japan Society for the Promotion of Science
2015
University of North Carolina at Chapel Hill
2006-2012
Tokyo Medical and Dental University
2004
City College of New York
1988
Despite widespread adoption of tissue clearing techniques in recent years, poor access to suitable light-sheet fluorescence microscopes remains a major obstacle for biomedical end-users. Here, we present descSPIM (desktop-equipped SPIM cleared specimens), low-cost ($20,000-50,000), low-expertise (one-day installation by non-expert), yet practical do-it-yourself microscope as solution this bottleneck. Even the most fundamental configuration enables multi-color imaging whole mouse brains and...
Lysyl oxidase (LOX), an amine critical for the initiation of collagen and elastin cross-linking, has recently been shown to regulate cellular activities possibly by modulating functions growth factors. In this study, we investigated interaction between LOX transforming factor-beta1 (TGF-beta1), a potent factor abundant in bone, effect on TGF-beta1 signaling, its potential mechanism. The specific binding mature was demonstrated immunoprecipitation glutathione S-transferase pulldown assay...
Significance Intraflagellar transport (IFT) plays a critical role in assembling primary cilia that mediate growth factor signaling. The disruption or dysfunction of IFT components can generate multiple diseases, including skeletal dysplasia. However, the mechanism by which regulates skeletogenesis remains elusive. In present study, we show neural crest-specific deletion gene encodes intraflagellar 20 (IFT20) mice compromises ciliogenesis and intracellular collagen, leading to osteopenia...
Abstract A comprehensive understanding of the extracellular matrix (ECM) is essential for developing biomimetic ECM scaffolds tissue regeneration. As periodontal ligament cell (PDLC)-derived has shown potential regeneration, it vital to gain a deeper its profile. Although PDLC-derived exhibits environment similar that (PDL) tissue, details molecular composition are lacking. Thus, using multiomics approach, we systematically analyzed cultured mouse and compared PDL as reference. Proteomic...
Abstract Endogenous collagen cross‐links influence cartilage biomechanical properties and resistance to degradation. Formalin fixation modifies residues forms new in a dose‐dependent manner. We tested the hypothesis that magnetization transfer (MT) effects T 2 depend on cross‐linking cartilage. These parameters were measured bovine nasal (BNC) prior fixation, after 9 weeks of immersion formalin solutions ranging concentration from 0% 10%, NaBH 3 CN reduction washing. decreased by 59.4% ±...
Abstract Mutations in the Family with sequence similarity (FAM) 20 gene family are associated mineralized tissue phenotypes humans. Among these genes, FAM20A mutations Amelogenesis Imperfecta (AI) gingival hyperplasia and nephrocalcinosis, while FAM20C cause Raine syndrome, exhibiting bone craniofacial/dental abnormalities. Although it has been demonstrated that syndrome associated- mutants prevented kinase activity secretion, overexpression of catalytically inactive D478A mutant was...
Objective: The purpose of the present study was to investigate reaction periodontal ligament excessive occlusal loading by observing histological changes and osteopontin induction. possibility ligand for receptor activator nuclear factor κB (RANKL) participation in induction also discussed. Background: precise mechanism breakdown remains unclear. We established an experimental model vivo . Osteopontin is known be produced upon mechanical considered induce migration osteoclasts resorption...
Fibrillar type I collagen, the predominant organic component in bone, is stabilized by lysyl oxidase (LOX)-initiated covalent intermolecular cross-linking, an important determinant of bone quality. However, impact collagen cross-linking on activity cells and subsequent tissue remodeling not well understood. In this study, we investigated effect cellular activities employing a loss-of-function approach, using potent LOX inhibitor, β-aminopropionitrile (BAPN). Osteoblastic (MC3T3-E1) were...
Type I collagen, a major extracellular component of the periodontal ligament (PDL), is post-translationally modified by series specific enzymes. Among collagen-modifying enzymes, lysyl oxidase (LOX) essential to initiate collagen cross-linking and hydroxylases (LHs) regulate pathways that are important for tissue mechanical properties. The purpose this study was investigate effects loading on expression enzymes subsequent changes in PDL. Primary human PDL cells were subjected 3D gel, gene...
The periodontal ligament (PDL) is a specialized connective tissue that provides structural support to the tooth and crucial for oral functions. mechanical properties of PDL are mainly derived from tissue-specific composition characteristics extracellular matrix (ECM). ECM also plays key roles in determining cell fate cellular microenvironment thus homeostasis. In present study, we determined comprehensive profile mouse molar using laser microdissection mass spectrometry-based proteomic...
Postmenopausal osteoporosis develops due to a deficiency of estrogen that causes decrease in bone mass and changes the macro- micro-architectural structure bone, leading loss mechanical strength an increased risk fracture. Although assessment mineral density (BMD) has been widely used as gold standard for diagnostic screening fracture risks, it accounts only part variation fragility; thus, is necessary consider other determinants strength. Therefore, we aimed comprehensively evaluate...
In a search of new, small leucine-rich repeat proteoglycan/protein (SLRP) family members, novel gene, nephrocan (NPN), has been identified. The gene consists three exons, and based on the deduced amino acid sequence, NPN 17 motifs unique cysteine-rich clusters both in N C termini, indicating that this belongs to new class SLRP family. mRNA was predominantly expressed kidney adult mice, during mouse embryogenesis, expression markedly increased 11-day-old embryos at time when early development...
The presence of matrix metalloproteinase-2 (MMP-2) in dentin has been reported, but its distribution and activity level mature human coronal are not well understood. purpose this study was to determine the MMP-2 relative demineralized dentin. Crowns twenty eight molars were sectioned into inner (ID), middle (MD), outer (OD) regions demineralized. extracted with 0.33 mol x L(-1) EDTA/2 xL(-1) guanidine-HCl, pH 7.4, concentration estimated enzyme-linked immunoabsorbant assay (ELISA). Further...
It is well accepted that cyclic mechanical loading induces osteoclastogenesis in periodontal tissue, but its molecular mechanisms are not understood, part because of a lack appropriate models. In this study, we investigated novel device allows to be performed well-controlled manner. Furthermore, by employing model, the effect on osteoclast recruitment tissue was described.By using newly developed device, 20 n (reference corresponding fracture hardness dietary pellets) and two excessive...