A5086548050- Congenital heart defects research
- Lymphatic System and Diseases
- Congenital Heart Disease Studies
- Tracheal and airway disorders
- Neonatal Respiratory Health Research
- Tissue Engineering and Regenerative Medicine
- Advanced Fluorescence Microscopy Techniques
- Ultrasound and Cavitation Phenomena
- Mesenchymal stem cell research
- Single-cell and spatial transcriptomics
- Renal and related cancers
- Combustion and Detonation Processes
- Laser-Plasma Interactions and Diagnostics
- Cell Image Analysis Techniques
Nippon Medical School
2022-2024
The University of Tokyo
1995-2022
Despite widespread adoption of tissue clearing techniques in recent years, poor access to suitable light-sheet fluorescence microscopes remains a major obstacle for biomedical end-users. Here, we present descSPIM (desktop-equipped SPIM cleared specimens), low-cost ($20,000-50,000), low-expertise (one-day installation by non-expert), yet practical do-it-yourself microscope as solution this bottleneck. Even the most fundamental configuration enables multi-color imaging whole mouse brains and...
Lymphatic vessels are crucial for tissue homeostasis and immune responses in vertebrates. Recent studies have demonstrated that lymphatic endothelial cells (LECs) arise from both venous sprouting (lymphangiogenesis) de novo production non-venous origins (lymphvasculogenesis), which is similar to blood vessel formation through angiogenesis vasculogenesis. However, the contribution of LECs networks considered be relatively small. Here, we identify Islet1 (Isl1)-expressing cardiopharyngeal...
The somatopleure is the amniotic primordium in amniote development, but its boundary to embryonic body at early stages and fate of cells constituting this structure are not well characterized. It also remains unclear how behave during demarcation between intra- extra-embryonic tissues. Here we identify cellular alignments, which indicate two streams towards sites dorsal closure ventral thoracic wall formation. A subpopulation mesodermal moving ventrally from somatopleural region adjacent...
The somatopleure serves as the primordium of amnion, an extraembryonic membrane surrounding embryo. Recently, we have reported that amniogenic somatopleural cells (ASCs) not only form amnion but also migrate into embryo and differentiate cardiomyocytes vascular endothelial cells. However, detailed differentiation processes final distributions these intra-embryonic ASCs (hereafter referred to iASCs) remain largely unknown.
ABSTRACT Lymphatic vessels are crucial for tissue homeostasis and immune responses in vertebrates. Recent studies have demonstrated that lymphatic endothelial cells (LECs) arise from both venous sprouting (lymphangiogenesis) de novo production non-venous origins (lymphvasculogenesis), which is similar to blood vessel formation through angiogenesis vasculogenesis. However, the contribution of LECs networks considered be relatively small. Here, we identify Islet1 ( Isl1 )-expressing...