A5016480257- Kruppel-like factors research
- Immune cells in cancer
- Cholesterol and Lipid Metabolism
- Muscle Physiology and Disorders
- Peroxisome Proliferator-Activated Receptors
- Genetic Syndromes and Imprinting
- Adipokines, Inflammation, and Metabolic Diseases
- Atherosclerosis and Cardiovascular Diseases
- Tea Polyphenols and Effects
- Cancer, Lipids, and Metabolism
- Extracellular vesicles in disease
- Adipose Tissue and Metabolism
- Lipoproteins and Cardiovascular Health
- Epigenetics and DNA Methylation
- Phytochemicals and Antioxidant Activities
- Neurogenetic and Muscular Disorders Research
- RNA Research and Splicing
- Immune Cell Function and Interaction
- Phagocytosis and Immune Regulation
- Tryptophan and brain disorders
- Cancer-related gene regulation
- Inflammasome and immune disorders
- Coffee research and impacts
- Congenital heart defects research
- Circular RNAs in diseases
Nippon Medical School
2018-2024
Tokyo Medical and Dental University
2005-2024
University of California, San Diego
2016
The University of Tokyo
2004-2011
National Institute of Infectious Diseases
2006
Bunkyo University
2004
Gunma University
1997-2001
Adipose tissue expression and circulating concentrations of monocyte chemoattractant protein-1 (MCP-1) correlate positively with adiposity. To ascertain the roles MCP-1 overexpression in adipose, we generated transgenic mice by utilizing adipocyte P2 (aP2) promoter (aP2-MCP-1 mice). These had higher plasma increased macrophage accumulation adipose tissues, as confirmed immunochemical, flow cytometric, gene analyses. Tumor necrosis factor-α interleukin-6 mRNA levels white non-esterified fatty...
Despite widespread adoption of tissue clearing techniques in recent years, poor access to suitable light-sheet fluorescence microscopes remains a major obstacle for biomedical end-users. Here, we present descSPIM (desktop-equipped SPIM cleared specimens), low-cost ($20,000-50,000), low-expertise (one-day installation by non-expert), yet practical do-it-yourself microscope as solution this bottleneck. Even the most fundamental configuration enables multi-color imaging whole mouse brains and...
Use of short interfering RNA (siRNA) is a promising new approach thought to have strong potential lead rapid development gene-oriented therapies. Here, we describe newly developed, systemically injectable siRNA vehicle, the "wrapsome" (WS), which contains and cationic lipofection complex in core that fully enveloped by neutral lipid bilayer hydrophilic polymers. WS protected from enzymatic digestion, providing long half-life systemic circulation. Moreover, siRNA/WS leaked blood vessels...
Bmal1 (encoded by Arntl gene) is a core circadian clock gene that regulates various genes involved in rhythm. Although expressed rhythmically macrophages, the role of regulation their cellular function remains insufficiently understood. Here, we report time-dependent inflammatory responses following Toll-like receptor 4 (TLR4) activation modulating enhancer activity. Global transcriptome analysis indicated deletion perturbed elicited TLR4 Kdo2-lipid A (KLA). recruitment NF-κB p65 was...
Krüppel-like factor 5 (Klf5) is a zinc-finger transcription that controls various biological processes, including cell proliferation and differentiation. We show Klf5 also an essential mediator of skeletal muscle regeneration myogenic During after injury (cardiotoxin injection), was induced in the nuclei differentiating myoblasts newly formed myofibers expressing myogenin vivo. Satellite cell-specific deletion severely impaired regeneration, myotube formation suppressed Klf5-deleted cultured...
Abstract Muscle regeneration requires the coordination of muscle stem cells, mesenchymal fibro-adipogenic progenitors (FAPs), and macrophages. How macrophages regulate paracrine secretion FAPs during recovery process remains elusive. Herein, we systemically investigated communication between CD206 + M2-like using a transgenic mouse model. Depletion or deletion macrophages-specific TGF-β1 gene induces myogenesis regeneration. We show that depletion activates activated secrete follistatin,...
Modulation of smooth muscle cell (SMC) phenotype plays a central role in neointima formation. We recently demonstrated that Am80, synthetic retinoic acid receptor alpha-specific agonist, inhibits the activity transcription factor KLF5, which is essential for formation after vascular injury. In present study, we aimed to further analyze mechanism by Am80 KLF5 and effects inhibiting on SMCs lesion formation, as well evaluate potential use prevention in-stent found inhibited both expression...
Proper resolution of inflammation is vital for repair and restoration homeostasis after tissue damage, its dysregulation underlies various noncommunicable diseases, such as cardiovascular metabolic diseases. Macrophages play diverse roles throughout initial inflammation, resolution, repair. Differential reprogramming reportedly required induction support the macrophage activation states. Here we show that a long noncoding RNA (lncRNA), lncFAO , contributes to in mice by promoting fatty acid...
Recent studies have shown that cellular metabolism is tightly linked to the regulation of immune cells. Here, we show activation cholesterol metabolism, involving uptake, synthesis, and autophagy/lipophagy, integral innate responses in macrophages. In particular, accumulation within endosomes lysosomes a hallmark dynamics elicited by Toll-like receptor 4 required for amplification myeloid differentiation primary response 88 (Myd88) signaling. Mechanistically, Myd88 binds via its CLR...
Macrophages are key regulators of skeletal muscle regeneration, yet the specific macrophage subpopulations responsible for this process and their cell-cell interactions remain insufficiently understood, as does mechanism underlying age-related impairment regeneration. We utilized single-cell RNA sequencing to identify transcriptionally distinct within from young (8-week-old) aged (24-month-old) mice. Among them, Mac_1 subpopulation interacted with satellite cells (MuSCs) promoted...
Abstract Proinflammatory cytokine production in macrophages involves multiple regulatory mechanisms, which are affected by environmental and intrinsic stress. In particular, accumulating evidence has suggested epigenetic control of macrophage differentiation function mainly vitro . SET domain, bifurcated 1 (Setdb1, also known as Eset) is a histone 3 lysine 9 (H3K9)-specific methyltransferase essential for early development embryos. Here we demonstrate that Setdb1 potently suppresses...
Significance Skeletal muscle atrophy occurs as a result of variety conditions including aging, disuse, and cachexia. In this study, we show that the transcription factor KLF5 is transiently up-regulated by metabolic disuse stimulations plays pivotal role in onset vitro vivo. physically interacts with Foxo1, together, they regulate Fbxo32 , key atrophy-inducing ubiquitin ligase. A synthetic retinoid, Am80, attenuates Moreover, publicly available data sets showed humans, expression...