A5009789102- Microtubule and mitosis dynamics
- Cancer-related Molecular Pathways
- Chronic Myeloid Leukemia Treatments
- Protein Kinase Regulation and GTPase Signaling
- TGF-β signaling in diseases
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- Axon Guidance and Neuronal Signaling
- Plant nutrient uptake and metabolism
- Genomics and Chromatin Dynamics
- Cellular Mechanics and Interactions
- Heat shock proteins research
- DNA Repair Mechanisms
- Wnt/β-catenin signaling in development and cancer
- Protein Degradation and Inhibitors
- Hippo pathway signaling and YAP/TAZ
- Melanoma and MAPK Pathways
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- PI3K/AKT/mTOR signaling in cancer
- Plant responses to water stress
- Mechanisms of cancer metastasis
- Extracellular vesicles in disease
- Moringa oleifera research and applications
- Cardiovascular Function and Risk Factors
Kyoto Pharmaceutical University
2020-2024
Chiba University
2011-2020
Nippon Medical School
2020
Proper resolution of inflammation is vital for repair and restoration homeostasis after tissue damage, its dysregulation underlies various noncommunicable diseases, such as cardiovascular metabolic diseases. Macrophages play diverse roles throughout initial inflammation, resolution, repair. Differential reprogramming reportedly required induction support the macrophage activation states. Here we show that a long noncoding RNA (lncRNA), lncFAO , contributes to in mice by promoting fatty acid...
Protein-tyrosine phosphorylation regulates a wide variety of cellular processes at the plasma membrane. Recently, we showed that nuclear tyrosine kinases induce global structure changes, which called chromatin structural changes. However, mechanisms are not fully understood. In this study identify protein kinase A anchoring 8 (AKAP8/AKAP95), associates with and matrix, as tyrosine-phosphorylated protein. Tyrosine AKAP8 is induced by several kinases, such Src, Fyn, c-Abl but Syk....
Abstract Src-family tyrosine kinases, which are expressed in various cell types, play critical roles signalling at the cytoplasmic side of plasma membrane through their lipid modifications. kinases cotranslationally myristoylated and posttranslationally palmitoylated amino-terminal region. The member Lyn contains a myristoylation site glycine-2 palmitoylation cysteine-3, whereas c-Src has but not any sites. However, little is known about role for modifications division. Here, we show that...
Krüppel-associated box-containing zinc finger proteins (KRAB-ZFPs) regulate a wide range of cellular processes. KRAB-ZFPs have KRAB domain, which binds to transcriptional corepressors, and DNA activate or repress gene transcription. Here, we characterize ZNF777, member KRAB-ZFPs. We show that ZNF777 localizes the nucleus inducible overexpression inhibits cell proliferation in manner dependent on its domain but independent domain. Intriguingly, drastically at low density slightly high...
Mitotic progression requires the precise formation of spindle microtubules based on mature centrosomes. During G2/M transition, centrosome maturation progresses, and associated bundle to form mitotic fibers capture chromosomes for alignment at cell equator. kinases-induced phosphorylation signaling is necessary these processes. Here, we identified SH2 domain-containing protein 4A (SH2D4A/PPP1R38) as a new regulator. SH2D4A knockdown delays progression. The time-lapse imaging analysis showed...
Activating transcription factor 2 (ATF2) and its homolog ATF7 are phosphorylated at Thr-69/Thr-71 Thr-51/Thr-53, respectively, by stress-activated MAPKs regulating their transcriptional functions in G1 S phases. However, little is known about the role of ATF2 G2/M phase. Here, we show that Cdk1-cyclin B1 phosphorylates Thr-51/Thr-53 from early prophase to anaphase absence any stress stimulation. Knockdown or decreases rate cell proliferation number cells M-phase. In particular, knockdown...
The mitotic spindle is the major piece of cellular machinery essential for faithful chromosome segregation. Whereas Fyn, a member Src-family kinases, known to be localized meiotic and microtubules, role Fyn in formation has not yet been completely elucidated. In this study, we studied effects on M-phase progression. Re-expression induced increases fluorescence intensity microtubules SYF cells having triple knock-out mutations c-Src, c-Yes, Fyn. Cold treatment results showed that maximum...
Cancer-type organic anion transporting polypeptide 1B3 (Ct-OATP1B3) mRNA is a variant isoform of the liver-type OATP1B3. Because Ct-OATP1B3 shows an excellent cancer-specific expression profile in colorectal cancer (CRC), and that its levels are associated with CRC prognosis, it holds potential to become useful detection diagnosis biomarker. While currently justified only at tissue level, if existence CRC-derived extracellular vesicles (EVs) validated, findings could enhance translational as...
The non-receptor-type tyrosine kinase c-Abl functions as a cytoplasmic signal transducer upon activation of cell-surface receptors. is also involved in DDR (DNA-damage response), which initiated the nucleus, whereas its molecular are not fully understood. In present study, we found that phosphorylates JunB, member AP-1 (activator protein 1) transcription factor family. Because JunB was suggested to be DDR, analysed role c-Abl-mediated phosphorylation DDR. We first sites and majorly at...
c-Abl is a non-receptor-type tyrosine kinase that regulates various cellular events, including cell proliferation, differentiation, and apoptosis, through phosphorylation of cytoplasmic nuclear targets. Although we showed induces histone deacetylation, the molecular mechanisms this phenomenon are largely unknown. Here, analyzed effect on expression deacetylase 1 (HDAC1), because was shown to be involved in maintenance protein levels HDAC1. Co-transfection HDAC1 with increased...
Anaplastic lymphoma kinase (ALK), a receptor-type tyrosine kinase, is involved in the pathogenesis of several cancers. ALK has been targeted with small molecule inhibitors for treatment different cancers, but absolute success remains elusive. In present study, effects on M phase progression were evaluated. Crizotinib, ceritinib, and TAE684 suppressed proliferation neuroblastoma SH-SY5Y cells concentration-dependent manner. At approximate IC50 concentrations, these caused misorientation...
When cells with excess DNA, such as tetraploid cells, undergo cell division, it can contribute to cellular transformation via asymmetrical chromosome segregation-generated genetic diversity. Cell cycle progression of is suppressed by large tumor suppressor 2 (LATS2) kinase-induced inhibitory phosphorylation the transcriptional coactivator Yes-associated protein (YAP). We recently reported that oncogene v-Src induces tetraploidy and promotes suppressing LATS2 activity. explore here mechanism...
Abstract The mammalian HSP105/110 family consists of four members, including Hsp105 and Apg‐1, which function as molecular chaperones. Recently, we reported that knockdown increases sensitivity to the DNA‐damaging agent Adriamycin but decreases microtubule‐targeting paclitaxel. However, whether other Hsp105/110 proteins have same functional property is unknown. Here, show Apg‐1 has different roles from in cell proliferation, division, drug sensitivity. We generated Apg‐1‐knockdown HeLa S3...
The insulin-like growth factor 1 receptor (IGF1R) is a receptor-type tyrosine kinase that transduces signals related to cell proliferation, differentiation, and survival. IGF1R expression often misregulated in tumor cells, but the relevance of this for cancer progression remains unclear. Here, we examined impact inhibition on division. We found siRNA-mediated knockdown from HeLa S3 cells leads M-phase delays. Although depletion causes partial exclusion FoxM1 nucleus, quantitative real-time...
Moringa oleifera Lamk. (M. oleifera) is an edible plant and used for traditional medicine formulation. Some bioactive phytochemicals found in M. leaves thus far were identified as quercetin, chlorogenic acid, astragalin, kaempferol. The flavonoid kaempferol was reported to induce apoptosis human HCT116 colon cancer cells. Here, we investigated the anti-proliferative activity present methanol extract from toward Fractionation of by gel filtration chromatography on Sephadex LH-20 enabled us...