A5052777358- Drug Transport and Resistance Mechanisms
- Amino Acid Enzymes and Metabolism
- Gout, Hyperuricemia, Uric Acid
- Metabolism and Genetic Disorders
- Ion Transport and Channel Regulation
- Epigenetics and DNA Methylation
- Alcohol Consumption and Health Effects
- Pharmacological Effects and Toxicity Studies
- Trace Elements in Health
- Polyamine Metabolism and Applications
- Case Reports on Hematomas
- Neuroscience and Neuropharmacology Research
- Liver Disease Diagnosis and Treatment
- Pancreatic function and diabetes
- Adenosine and Purinergic Signaling
- Pharmacological Effects of Natural Compounds
- Electrolyte and hormonal disorders
- Pregnancy and Medication Impact
- Porphyrin Metabolism and Disorders
- Diabetes Treatment and Management
- Barrier Structure and Function Studies
- Carcinogens and Genotoxicity Assessment
- Neuroinflammation and Neurodegeneration Mechanisms
- Peroxisome Proliferator-Activated Receptors
- Renal and related cancers
Dokkyo Medical University
2015-2025
Chiba University
2016-2025
Dokkyo University
2019-2021
Kyorin University
2003-2019
Pharmac
2019
Kobe University
2014
Kyoto University
2014
Institute of Pharmacology
2010
Weatherford College
2007
Osaka University
2006
Hyperuricemia is a significant factor in variety of diseases, including gout and cardiovascular diseases. Although renal excretion largely determines plasma urate concentration, the molecular mechanism handling remains elusive. Previously, we identified major reabsorptive transporter, URAT1 (SLC22A12), on apical side proximal tubular cells. However, it not known how taken up by exits from cell to systemic circulation. Here, report that sugar transport facilitator family member protein GLUT9...
Most tumor cell membranes overexpress l ‐type amino acid transporter 1, while normal contain 2; both are Na + ‐independent transporters. Therefore, compounds that selectively inhibit 1 offer researchers with a novel cancer molecular target. Synthetic chemistry efforts and in vitro screening have produced variety of possessing high l‐ type selectivity; KYT‐0353 was one such compound. The present studies illustrate inhibited 14 C‐leucine uptake growth human colon cancer‐derived HT‐29 cells; IC...
A cDNA that encodes a novel Na+-independent neutral amino acid transporter was isolated from FLC4 human hepatocarcinoma cells by expression cloning. When expressed in Xenopus oocytes, the encoded protein designated LAT3 (L-type 3) transported acids such as l-leucine, l-isoleucine, l-valine, and l-phenylalanine. The LAT3-mediated transport inhibited 2-aminobicyclo[2.2.1]heptane-2-carboxylic acid, consistent with properties of system L. Distinct already known L transporters LAT1 LAT2, which...
The tubular secretion of diuretics in the proximal tubule has been shown to be critical for action drugs. To elucidate molecular mechanisms excretion diuretics, we have elucidated interactions human organic anion transporters (hOATs) with using cells stably expressing hOATs. Diuretics tested were thiazides, including chlorothiazide, cyclothiazide, hydrochlorothiazide, and trichlormethiazide; loop bumetanide, ethacrynic acid, furosemide; carbonic anhydrase inhibitors, acetazolamide...
l-Carnitine is an essential component of mitochondrial fatty acid β-oxidation and plays a pivotal role in the maturation spermatozoa within male reproductive tract. Epididymal plasma contains highest levels l-carnitine found human body, initiation sperm motility occurs parallel to increase epididymal lumen. Using specific carrier, epithelium secretes into lumen by active transport mechanism; however, structure-activity relationship comprising carnitine-permeation pathway poorly understood....
The urate-anion exchanger URAT1 is a member of the organic anion transporter (OAT) family that regulates blood urate level in humans and targeted by uricosuric antiuricosuric agents (Enomoto, A., Kimura, H., Chairoungdua, Shigeta, Y., Jutabha, P., Cha, S. Hosoyamada, M., Takeda, Sekine, T., Igarashi, Matsuo, Kikuchi, Oda, Ichida, K., Hosoya, Shimotaka, Niwa, Kanai, Endou, H. (2002) Nature 417, 447–452). expressed only kidney, where it thought to participate tubular reabsorption. We found...
The purpose of this study was to elucidate the interactions human organic anion transporters (hOATs) and cation (hOCTs) with nonsteroidal anti-inflammatory drugs (NSAIDs) using cells stably expressing hOATs hOCTs. NSAIDs tested were acetaminophen, acetylsalicylate, salicylate, diclofenac, ibuprofen, indomethacin, ketoprofen, mefenamic acid, naproxen, piroxicam, phenacetin, sulindac. These inhibited uptake mediated by hOAT1, hOAT2, hOAT3, hOAT4. By comparing IC<sub>50</sub> values for hOATs,...
The evolutionary loss of hepatic urate oxidase (uricase) has resulted in humans with elevated serum uric acid (urate). Uricase may have been beneficial to early primate survival. However, an predisposed man hyperuricemia, a metabolic disturbance leading gout, hypertension, and various cardiovascular diseases. Human levels are largely determined by reabsorption secretion the kidney. Renal is controlled via two proximal tubular transporters: apical URAT1 (SLC22A12) basolateral URATv1/GLUT9...
Abstract Activation of T cells accompanies remarkable enhancement metabolism. Sufficient and continuous nutrient supply is therefore important to support immune reaction in cells. However, the mechanism promotion incorporation activated has not been elucidated. In this study, we show that L-type amino acid transporter 1 (LAT1) a major for essential acids into human CD3/CD28 stimulation primary triggered dramatic induction LAT1 expression mediated by NF-κB AP-1. Functional disturbance...
Amino acid transporters are essential for maintenance and proliferation of both normal transformed cells. In the present study, L‐type amino‐acid transporter 1 (LAT1) immunoreactive expression was investigated in gastric carcinomas, comparison with adenomas non‐neoplastic lesions, using our recently developed novel monoclonal antibody. a total 87 cases advanced cancer, high LAT1 observed carcinoma cells, predominantly at plasma membranes greater intensity non‐scirrhous than scirrhous...
Tubular secretion of the organic cation, creatinine, limits its value as a marker glomerular filtration rate (GFR) but molecular determinants this pathway are unclear. The anion transporters, OAT1 and OAT3, expressed on basolateral membrane proximal tubule transport anions also neutral compounds cations. Here, we demonstrate specific uptake creatinine into mouse mOat1- mOat3-microinjected Xenopus laevis oocytes at concentration 10 μM (i.e., similar to physiological plasma levels), which was...
The liver plays an important role in the elimination of endogenous and exogenous lipophilic organic compounds from body, which is mediated by various carrier proteins that differ substrate specificity kinetic properties. Here, we have characterized a novel member anion transporter family (SLC22) isolated human liver. named 7 (OAT7/ SLC22A9) showed 35% to 46% identities those other transporters SLC22 family. When expressed Xenopus oocytes, OAT7 Na+-independent, high-affinity transport...
SLC17A1 protein (NPT1) is the first identified member of SLC17 phosphate transporter family and mediates transmembrane cotransport Na(+)/P(i) in oocytes. Although this believed to be a renal polyspecific anion exporter, its transport properties are not well characterized. Here, we show that proteoliposomes containing purified various organic anions such as p-aminohippuric acid acetylsalicylic (aspirin) an inside positive membrane potential (Deltapsi)-dependent manner. We found NPT1 also...
Hyperuricaemia is a significant factor in variety of diseases, including gout and cardiovascular diseases. The kidney plays dominant role maintaining plasma urate levels through the excretion process. Human renal transporter URAT1 thought to be an essential molecule that mediates reabsorption on apical side proximal tubule. In this study pharmacological characteristics clinical implications were elucidated.Madin-Darby canine (MDCK) cells stably expressing (MDCK-URAT1) established examined...
Perfluorooctanoic acid, an environmental contaminant, is found in both wild animals and human beings. There are large species sex differences the renal excretion of perfluorooctanoic acid. In present study, we aimed to characterize organic anion transporters 1-3 (OAT1-3) beings rats investigate whether elimination kinetics acid from kidneys can be attributed affinities these for We used (h) rat (r) OAT transient expression cell systems measured [(14)C] transport activities. Both OAT1 OAT3...