A5101876495- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Metabolism and Genetic Disorders
- Ovarian function and disorders
- Sexual Differentiation and Disorders
- Neurobiology and Insect Physiology Research
- Prenatal Screening and Diagnostics
- Invertebrate Immune Response Mechanisms
- Amino Acid Enzymes and Metabolism
- Assisted Reproductive Technology and Twin Pregnancy
- Reproductive Health and Technologies
- Transition Metal Oxide Nanomaterials
- Epigenetics and DNA Methylation
- Pancreatic function and diabetes
- Neurogenetic and Muscular Disorders Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Insect Utilization and Effects
- Biochemical Analysis and Sensing Techniques
- Silicone and Siloxane Chemistry
- Caveolin-1 and cellular processes
- Advanced Sensor and Energy Harvesting Materials
- Endometriosis Research and Treatment
- RNA modifications and cancer
- Insect symbiosis and bacterial influences
- Protease and Inhibitor Mechanisms
Sungkyunkwan University
2003-2022
Samsung Medical Center
2022
Pusan National University
1999-2020
Chung-Ang University
2020
Cheil General Hospital and Women's Healthcare Center
2006-2018
Dankook University
2016-2018
Catholic Kwandong University
2007-2012
Kyorin University
2002-2008
Samsung (South Korea)
2003
Previously, we reported the molecular cloning of cDNA for prophenoloxidase activating factor‐I (PPAF‐I) that encoded a member serine proteinase group with disulfide‐knotted motif at N‐terminus and trypsin‐like catalytic domain C‐terminus [Lee, S.Y., Cho, M.Y., Hyun, J.H., Lee, K.M., Homma, K.I., Natori, S., Kawabata, S.I., Iwanaga, S. & B.L. (1998) Eur. J. Biochem . 257 , 615–621]. PPAF‐I is directly involved in activation pro‐phenoloxidase (pro‐PO) by limited proteolysis overall...
Tyrosinase is a copper-binding enzyme involved in melanin biosynthesis. However, the detailed structure of human tyrosinase has not yet been solved, along with identification key sites responsible for its catalytic activity. We used site-directed mutagenesis to identify residues critical copper binding tyrosinase. Seven histidine mutants two were generated, and activities characterised. The tyrosine hydroxylase CuA site approximately 50% lower than those wild-type tyrosinase, while dopa...
We identified a novel Na<sup>+</sup>-independent acidic amino acid transporter designated AGT1 (aspartate/glutamatetransporter 1). exhibits the highest sequence similarity (48% identity) to small neutral Asc (asc-type transporter)-2 member of heterodimeric family presumed be associated with unknown heavy chains (Chairoungdua, A., Kanai, Y., Matsuo, H., Inatomi, J., Kim, D. K., and Endou, H. (2001) <i>J. Biol. Chem.</i> 276, 49390–49399). The cysteine residue responsible for disulfide bond...
This study was performed to evaluate and compare threshold sperm parameters DNA fragmentation index (DFI), further analyzed whether DFI could be predicted from in men with varicocele. A total of 157 semen samples underwent both analysis (SDF) testing Sperm were assessed using the World Health Organization guidelines. SDF Halosperm kit. results compared. The overall parameter showed normal values; however, morphology value at lower limit normal. High associated significantly motility...
This study was performed to compare the efficiency of slow freezing and vitrification based on survival, development blastocysts, cell numbers blastocysts. Changes in embryonic gene expression fresh frozen-thawed embryos were also examined.Eight-cell stage collected from superovulated female BDF1 mice. The randomly divided into three groups. One group maintained as controls (n=42), one frozen by (n=43), cooled (n=43). After thawing or cooling, survival rates, blastocyst, inner mass (ICM)...
One of the biological functions activated phenoloxidase in arthropods is synthesis melanin around invaded foreign materials. However, little known about how synthesizes at molecular level. Even though it has been suggested that quinone derivatives generated by might use endogenous protein components for arthropods, there no report engaged induced phenoloxidase. In this study, to isolate and characterize proteins involved synthesis, we prepared vitro prophenoloxidase activating solution...
Recently, we reported two novel early‐staged encapsulation‐relating proteins (56 kDa and 48 ERPs) isolated from the hemolymph of coleopteran insect, Tenebrio molitor larvae [Cho et al. (1999) Eur. J. Biochem. (in press)]. Here, a cDNA clone for another protein (86 kDa) was isolated. We found that 86 shows high homology with insect diapause 1. The localized in fat body hemolymph, but not hemocyte lysate. A significant level detected pre‐pupae stage, it decreased rapidly at late pupae, no...
This study was performed to evaluate the independent influence of paternal age affecting embryo development and pregnancy using testicular sperm extraction (TESE)-intracytoplasmic injection (ICSI) in obstructive azoospermia (OA) nonobstructive (NOA). Paternal patients were divided into following groups: ≤30 years, 31–35 36–40 41–45 years ≥46 years. There no differences rates fertilisation or quality according maternal age. However, clinical implantation significantly lower between those...
Objective The aim of this study was to evaluate the influence maternal age on fertilization, embryo quality, and clinical pregnancy in patients undergoing intracytoplasmic sperm injection (ICSI) using testicular from partners with azoospermia. Methods A total 416 ICSI cycles spermatozoa obstructive azoospermia (OA, n=301) non-obstructive (NOA, n=115) were analyzed. Female divided into following groups: 27 31 years, 32 36 37 41 years. rates high-quality embryos, pregnancy, delivery compared...
Ornithine transcarbamylase (OTC) deficiency is an X-linked co-dominant disorder. A couple, with a previous history of neonatal death and therapeutical termination due to OTC deficiency, was referred our center for preimplantation genetic diagnosis (PGD). The female partner has nonsense mutation in the exon 9 gene (R320X). We carried out nested polymerase chain reaction (PCR) R320X fluorescence situ hybridization (FISH) aneuploidy screening. Among total 11 embryos, two blastomeres per embryo...
The pre-diagnostic test for preimplantation genetic diagnosis (PGD) of long-chain 3-hydroxyacyl-CoA dehydrogenase deficiency was performed by polymerase chain reaction (PCR) and direct sequencing hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme thiolase/enoyl-Coenzyme hydratase (HADHA) gene. We obtained unexpected genotyping results HADHA gene allele drop-out in the analysis patients' genomic DNA samples with a referred PCR primer set. Upon further re-designed set, we found novel...