Assisted reproductive techniques involving isolation, culture, and transplantation of spermatogonial stem cells (SSCs) have the potential to create transgenic livestock treat male infertility caused by cancer treatments such as chemotherapy or radiation. Because may need be preserved for several years before reintroduction patients' testes, efficient SSC cryopreservation developed. SSCs can reinitiate spermatogenesis in recipient testes after freezing; however, optimal protocols not been...

Growth hormone and its mediator, insulin-like growth factor-1 (IGF-1), have been suggested to exert gonadotropic actions in both humans animals. The present study was conducted assess the relationship between serum IGF-1 concentration, seminal plasma sperm parameter abnormalities.A total of 79 men were enrolled this from December 2011 July 2012 prospectively analyzed. Patient parameters analyzed included age, body mass index, smoking status, urological history, fertility history. Patients...

This study aimed to compare the clinical outcomes between in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) sibling oocytes. Additionally, we evaluated whether implementation of split insemination contributed an increase number ICSI procedures.A total 571 cycles 555 couples undergoing were included this study. Among them, 512 (89.7%) a couple's first IVF cycle. The patients under 40 years age at least 10 oocytes retrieved all cycles. Sibling randomly allocated or...

This study was performed to evaluate and compare threshold sperm parameters DNA fragmentation index (DFI), further analyzed whether DFI could be predicted from in men with varicocele. A total of 157 semen samples underwent both analysis (SDF) testing Sperm were assessed using the World Health Organization guidelines. SDF Halosperm kit. results compared. The overall parameter showed normal values; however, morphology value at lower limit normal. High associated significantly motility...

Artificial oocyte activation (AOA) is an effective method to avoid total fertilization failure in human vitro fertilization-embryo transfer (IVF-ET) cycles. AOA performed using a calcium ionophore can induce oscillation oocytes and initiate the process. We evaluated usefulness of with cases previous cycles severe male factor infertility patients non-motile spermatozoa after pentoxifylline (PF) treatment.The present study describes 29 intracytoplasmic sperm injection (ICSI)-AOA involving at...

This study was performed to evaluate testicular sperm chromatin condensation using aniline blue-eosin (AB-E) staining and its effects on IVF-ET.Chromatin analyzed AB-E in 27 cases of extraction. There were 19 obstructive azoospermia (OA) 8 non-obstructive (NOA) IVF-ET. Mature heads stained red-pink whereas immature dark blue. The percentage calculated from the ratio number total analyzed.The overall percentages OA NOA 31.1±11.2% 26.3±14.4%, respectively. fertilization rate significant higher...

This study was performed to assess and compare the outcomes of testicular sperm extraction (TESE)-intracytoplasmic injection (ICSI) using spermatozoa from fresh frozen tissue men with subgroups non-obstructive azoospermia (NOA). A total 110 cycles TESE-ICSI were performed. Patients classified into one following NOA subgroups: hypospermatogenesis (HS), maturation arrest (MA), or Sertoli cell-only syndrome (SCO). Laboratory (fertilization, cleavage stage embryo, good quality embryo) clinical...

A retrospective study was conducted in a large population presenting with infertility to determine whether sperm quality has changed Korea the last 10 years. We reviewed concentration, motility and semen volume 22,249 men from whom collected our laboratory between January 1989 April 1998 analysed according WHO (1987 ) guidelines. Mean age of 32 years (range 21–40). Data were healthy infertility. The mean concentration 60.5 ×10 6 /mL 1998. There no statistically significant difference for...

The majority of embryo transfers (ETs) to date have been performed on day 3 reduce the potential risk developmental arrest in vitro cultured embryos before ET. Development sequential media has significantly improved culture conditions and allowed blastocyst transfer 5. While 5 ET provides higher clinical pregnancy outcomes with reduced risks multiple pregnancies, it still IVF embryos. aim this study was evaluate 4 ETs compare efficacy ET.From 2006 2009, a total 747 fresh IVF-ET cycles were...

The presence of sperm-head vacuoles has been suspected to be deleterious the outcomes assisted reproductive technology (ART). It is difficult accurately distinguish morphologically abnormal sperm with under a light microscope. This study was performed analyze result observation using Papanicolaou staining microscope and whether male partner's age affects these vacuoles.Sperm morphology evaluated observed (400×) in 980 men. normal divided into three categories (group A, <4% morphology; group...

Follicle stimulating hormone (FSH) is important for controlling spermatogenesis through binding with its receptor. However, little information available on mutations of the FSH and receptor gene in infertile men. To study genetic defects, which caused problems spermatogenesis, we screened point men high serum concentrations. Seventy male patients FHS levels (> 12 mIU/ml) were each 10 exons gene, using genomic DNA PCR a single-strand conformation polymorphism (SSCP) analysis. From this study,...

This study was performed to evaluate the independent influence of paternal age affecting embryo development and pregnancy using testicular sperm extraction (TESE)-intracytoplasmic injection (ICSI) in obstructive azoospermia (OA) nonobstructive (NOA). Paternal patients were divided into following groups: ≤30 years, 31–35 36–40 41–45 years ≥46 years. There no differences rates fertilisation or quality according maternal age. However, clinical implantation significantly lower between those...

Objective The aim of this study was to evaluate the influence maternal age on fertilization, embryo quality, and clinical pregnancy in patients undergoing intracytoplasmic sperm injection (ICSI) using testicular from partners with azoospermia. Methods A total 416 ICSI cycles spermatozoa obstructive azoospermia (OA, n=301) non-obstructive (NOA, n=115) were analyzed. Female divided into following groups: 27 31 years, 32 36 37 41 years. rates high-quality embryos, pregnancy, delivery compared...

This study was conducted to find the relative risk of semen abnormality with respect smoking history and obesity.Subfertile or infertile men were enrolled in this from July 2010 June 2011. All participants provided their cigarette use information, self-reported weight, height, analysis, physical examination, sexually transmitted disease status. None patients had any specific pathological reason for infertility. Semen defined as a condition which one more parameters did not satisfy World...

To clarify (phospho-) glycogen synthase kinase-3 (GSK3) isoform variants in the germline and soma of human testes spermatozoa. GSK3 normospermatogenic Sertoli cell-only (SCO) testicular biopsies spermatozoa were examined. In testes, GSK3α GSK3β 1 2 different low complexity region (LCR) expressed their levels decreased SCO testes. variant 3 was only as well GSK3α, dominant isoforms found spermatogonia markedly meiotic germ cells which dominant. p-GSK3α/β marginal early spermatocytes. GSK3α/β...

비선택성 제초제인 글루포시네이트암모늄액제를 농작업자의 오판에 의한 오용으로 사과나무에 직접 살포된 경우 품종별, 수령별로 나타나는 약해반응과 수확에 미치는 영향을 조사하였다. 사과나무 품종에 따라 약해정도가 다르게 나타났다. 약제살포 당해연도에는 산사 > 쓰가루 홍로 후지 순으로, 다음해에는 순으로 피해가 나타났으나, 그 피해정도는 당년에 비해 경미하였다. 이듬해, 후지에서는 꽃과 착과정도에서만 30%정도 감소하였으나 쓰가루, 홍로, 산사에서는 개화상태, 착과율 등에서 전혀 피해증상이 관찰되지 않았다. 약해를 받은 조생종 쓰가루와 중만생종후지 대상으로 각각 수확기 10일전에 사과 중 잔류량을 검사한 결과, 잔류량은 검출한계(0.04ppm) 미만이었다. 그리고 오용 2년 후(2009년)에는 후지, 모두에서 약해증상이 나타나지 This study was conducted to investigate the phytotoxic reaction and impact on growth...

In conventional in vitro embryo production, oocytes are obtained from various ovaries, and donor characteristics, such as pedigree type, meat quality, or quantity, not considered. The aim of this study was to examine the effects type quality quantity slaughterhouse cows on oocyte recovery, development, pregnancy rates. ovaries individual Korean native were a slaughterhouse. ear tag numbers 24 h after slaughter, data compared according (no registration third bloodline registration),...