A5004964979- ATP Synthase and ATPases Research
- Drug Transport and Resistance Mechanisms
- Mitochondrial Function and Pathology
- Amino Acid Enzymes and Metabolism
- Neuroscience and Neuropharmacology Research
- Adenosine and Purinergic Signaling
- Trace Elements in Health
- Biochemical and Molecular Research
- Ion Transport and Channel Regulation
- Metabolism and Genetic Disorders
- Cellular transport and secretion
- Receptor Mechanisms and Signaling
- DNA and Nucleic Acid Chemistry
- Advanced Electron Microscopy Techniques and Applications
- RNA and protein synthesis mechanisms
- Pharmacological Effects and Toxicity Studies
- RNA Interference and Gene Delivery
- Genetics and Neurodevelopmental Disorders
- Ion channel regulation and function
- Enzyme Structure and Function
- Pediatric Hepatobiliary Diseases and Treatments
- Lipid Membrane Structure and Behavior
- Malaria Research and Control
- Polyamine Metabolism and Applications
- Protein Interaction Studies and Fluorescence Analysis
Okayama University
2013-2024
Osaka City University
2010
The University of Texas Southwestern Medical Center
2008
University of Virginia Health System
2002-2006
University of Virginia
2000-2006
Advanced Science Research Center
2005
Osaka University
1991-2000
Japan Science and Technology Agency
2000
Osaka Research Institute of Industrial Science and Technology
1991-2000
Science and Technology Corporation (United States)
2000
In mammals, toxic electrolytes of endogenous and exogenous origin are excreted through the urine bile. Before excretion, these compounds cross numerous cellular membranes in a transporter-mediated manner. However, protein transporters involved final excretion step poorly understood. Here, we show that MATE1, human mouse orthologue multidrug toxin extrusion family conferring resistance on bacteria, is primarily expressed kidney liver, where it localized to luminal urinary tubules bile...
F 0 1 , found in mitochondria or bacterial membranes, synthesizes adenosine 5′-triphosphate (ATP) coupling with an electrochemical proton gradient and also reversibly hydrolyzes ATP to form the gradient. An actin filament connected a c subunit oligomer of was able rotate by using energy hydrolysis. The rotary torque produced reached about 40 piconewton-nanometers, which is similar that generated γ motor. These results suggest subunits together during hydrolysis synthesis. Thus, coupled...
ATP is a major chemical transmitter in purinergic signal transmission. Before secretion, stored secretory vesicles found cells. Although the presence of active transport mechanisms for has been postulated long time, proteins responsible its vesicular accumulation remains unknown. The transporter encoded by human and mouse SLC17A9 gene, novel member an anion family, was predominantly expressed brain adrenal gland. bovine counterparts were associated with chromaffin granules. Proteoliposomes...
Abstract Ascorbate is an antioxidant and coenzyme for various metabolic reactions in vivo . In plant chloroplasts, high ascorbate levels are required to overcome photoinhibition caused by strong light. However, synthesized the mitochondria molecular mechanisms underlying transport into chloroplasts unknown. Here we show that AtPHT4;4, a member of phosphate transporter 4 family Arabidopsis thaliana , functions as transporter. vitro analysis shows proteoliposomes containing purified AtPHT4;4...
Despite the high incidence of neuropathic and inflammatory pain worldwide, effective drugs with few side effects are currently unavailable for treatment chronic pain. Recently, researchers have proposed that inhibitors purinergic chemical transmission, which plays a key role in pathological response, may allow targeted However, such therapeutic analgesic agents yet to be developed. In present study, we demonstrated clodronate, first-generation bisphosphonate comparatively fewer than...
ATPase activity associated with P-glycoprotein (Pgp) is characterized by three drug-dependent phases: basal (no drug), drug-activated, and drug-inhibited. To understand the communication between drug-binding sites ATP hydrolytic sites, we performed steady-state thermodynamic analyses of hydrolysis in presence absence transport substrates. We used purified human Pgp (ABCB1, MDR1) expressed Saccharomyces cerevisiae (Figler, R. A., Omote, H., Nakamoto, K., Al-Shawi, M. K. (2000) Arch. Biochem....
Aspartate is an excitatory amino acid that costored with glutamate in synaptic vesicles of hippocampal neurons and synaptic-like microvesicles (SLMVs) pinealocytes exocytosed stimulates neighboring cells by binding to specific cell receptors. Although evidence increasingly supports the occurrence aspartergic neurotransmission, this process still debated because mechanism for vesicular storage aspartate unknown. Here, we show sialin, a lysosomal H + /sialic cotransporter, present pineal...
Vesicular glutamate transporters (VGLUTs) are responsible for the vesicular storage of l-glutamate and play an essential role in glutamatergic signal transmission central nervous system. The molecular mechanism transport remains unknown. Here, we established a novel vitro assay procedure, which includes purification wild mutant VGLUT2 their reconstitution with purified bacterial FoF1-ATPase (F-ATPase) into liposomes. Upon addition ATP, proteoliposomes facilitated uptake membrane potential...
SLC17A1 protein (NPT1) is the first identified member of SLC17 phosphate transporter family and mediates transmembrane cotransport Na(+)/P(i) in oocytes. Although this believed to be a renal polyspecific anion exporter, its transport properties are not well characterized. Here, we show that proteoliposomes containing purified various organic anions such as p-aminohippuric acid acetylsalicylic (aspirin) an inside positive membrane potential (Deltapsi)-dependent manner. We found NPT1 also...
Spermine and spermidine act as neuromodulators upon binding to the extracellular site(s) of various ionotropic receptors, such N-methyl-d-aspartate receptors. To gain access polyamines synthesized in neurons astrocytes are stored secretory vesicles released depolarization. Although vesicular storage is mediated an ATP-dependent, reserpine-sensitive fashion, transporter responsible for this process remains unknown. SLC18B1 fourth member SLC18 family, which includes monoamine transporters...
Alkaloids play a key role in higher plant defense against pathogens and herbivores. Following its biosynthesis root tissues, nicotine, the major alkaloid of Nicotiana species, is translocated via xylem transport toward accumulation sites, leaf vacuoles. Our transcriptome analysis methyl jasmonate-treated tobacco BY-2 cells identified several multidrug toxic compound extrusion (MATE) transporter genes. In this study, we characterized MATE gene, tabacum jasmonate-inducible 2 (Nt-JAT2), which...
Significance Malaria caused by Plasmodium falciparum is a severe infectious disease with high mortality and morbidity rates worldwide. Chloroquine (CQ) widely used antimalarial agent, but the emergence spread of CQ-resistant parasites growing global health problem. Although its physiological relevance remains unknown, P. CQ resistance transporter (PfCRT) confers through egress from digestive vacuoles . To address this issue, recombinant CQ-sensitive or PfCRT proteins were purified their...
The rotation of the γ-subunit has been included in binding-change mechanism ATP synthesis/hydrolysis by proton synthase (F O F 1 ). Escherichia coli was engineered for studies such that its hydrolysis and synthesis activity is similar to wild type. A fluorescently labeled actin filament connected sector rotated on addition ATP. This progress enabled us analyze γM23K (the Met-23 replaced Lys) mutant, which defective energy coupling between catalysis translocation. We found produced...
The structure of Escherichia coli F 0 1 ‐ATPase (ATP synthase), and its sector reconstituted in lipid membranes was analyzed using atomic force microscopy (AFM) by tapping‐mode operation. majority ‐ATPases were visualized as spheres with a calculated diameter , height from the membrane surface. sectors two different ring‐like structures (one central mass other hollow depth) outer . images possibly represent opposite orientations complex membranes. projections both suggest inherently...
Abstract Multidrug resistance (MDR) poses a major challenge to medicine. A principle cause of MDR is through active efflux by transporters situated in the bacterial membrane. Here we present crystal structure facilitator superfamily (MFS) drug/H + antiporter MdfA from Escherichia coli an outward open conformation. Comparison with inward facing (drug binding) state shows that, addition expected change relative orientations N- and C-terminal lobes antiporter, conformation TM5 kinked twisted....
Neuroendocrine cells store ATP in secretory granules and release it along with hormones that may trigger a variety of cellular responses process called purinergic chemical transmission. Although the vesicular nucleotide transporter (VNUT) has been shown to be involved storage ATP, its physiological relevance vivo is far less well understood. In Vnut knockout (Vnut(-/-)) mice, we found loss functional VNUT adrenal chromaffin insulin islets Langerhans led several significant effects. Vesicular...
Abstract LETM1 is a mitochondrial inner membrane protein that required for maintaining the morphology and cristae structures, regulates ion homeostasis. Here we report role of in organization structures. We identified four amino acid residues human are crucial complementation growth deficiency caused by gene deletion yeast orthologue. Substituting with alanine disrupts correct assembly complex containing prevents changes induced exogenous expression. Moreover, shapes membranes...