A5059937432- Biochemical Analysis and Sensing Techniques
- Olfactory and Sensory Function Studies
- Advanced Chemical Sensor Technologies
- Adenosine and Purinergic Signaling
- Digestive system and related health
- Cancer Cells and Metastasis
- Pancreatic function and diabetes
- RNA and protein synthesis mechanisms
- Regulation of Appetite and Obesity
- Neuroscience and Neuropharmacology Research
- Fibroblast Growth Factor Research
- Neuroscience of respiration and sleep
- Immune Cell Function and Interaction
- Mesenchymal stem cell research
- Gut microbiota and health
- Wound Healing and Treatments
- Pain Mechanisms and Treatments
- Tissue Engineering and Regenerative Medicine
- Wnt/β-catenin signaling in development and cancer
- Cytokine Signaling Pathways and Interactions
- Cleft Lip and Palate Research
- IL-33, ST2, and ILC Pathways
- Proteoglycans and glycosaminoglycans research
- Pressure Ulcer Prevention and Management
- Peroxisome Proliferator-Activated Receptors
Tokyo University of Agriculture
2015-2025
Ajinomoto (Japan)
2010-2017
Ajinomoto (United States)
2009-2011
Suzuki (Japan)
2010
Institute of Life Sciences
2010
Icahn School of Medicine at Mount Sinai
2007
Tokyo Metropolitan Institute of Medical Science
2002-2004
The University of Tokyo
1997-1998
Nagoya University
1995-1997
Tokyo Metropolitan Institute of Gerontology
1995
Mouse mothers transfer metabolic mode Obesity and diseases tend to go together, humans who become obese are also prone type 2 diabetes cardiovascular problems. Starting with the observation that offspring of germ-free mice tended on high-fat diets, Kimura et al. investigated how presence microbiota might be protective in (see Perspective by Ferguson). Short-chain fatty acids (SCFAs) from known suppress insulin signaling reduce fat deposition adipocytes. Further experiments showed SCFAs...
Significance Taste tissue regenerates continuously throughout the life span in mammals. Here, using lineage tracing and a culture system, we show that leucine-rich repeat-containing G protein-coupled receptor 5-expressing 6-expressing taste stem/progenitor cells generate mature vivo ex vivo. Importantly, our studies single-progenitor can all cell types differentiated form absence of innervation. This model mimics development bud papillae, recapitulates process renewal from adult stem to...
Abstract Activation of purinergic receptors in the spinal cord by extracellular ATP is essential for neuropathic hypersensitivity after peripheral nerve injury (PNI). However, cell type responsible releasing within PNI unknown. Here we show that increases expression vesicular nucleotide transporter (VNUT) cord. Extracellular content ([ATP] e ) was increased PNI, and this increase suppressed exocytotic inhibitors. Mice lacking VNUT did not PNI-induced [ATP] had attenuated hypersensitivity....
Until recently, reliable markers for adult stem cells have been lacking many regenerative mammalian tissues. Lgr5 (leucine-rich repeat-containing G-protein-coupled receptor 5) has identified as a marker in intestine, stomach, and hair follicle; Lgr5-expressing give rise to all types of these Taste epithelium also regenerates constantly, yet the identity taste remains elusive. In this study, we found that is strongly expressed at bottom trench areas base circumvallate (CV) foliate papillae...
Despite the high incidence of neuropathic and inflammatory pain worldwide, effective drugs with few side effects are currently unavailable for treatment chronic pain. Recently, researchers have proposed that inhibitors purinergic chemical transmission, which plays a key role in pathological response, may allow targeted However, such therapeutic analgesic agents yet to be developed. In present study, we demonstrated clodronate, first-generation bisphosphonate comparatively fewer than...
Significance Despite improved understanding of cellular and molecular events mediating type 2 immune responses in the gut, how these changes facilitate worm expulsion remains largely unknown. We identified pyroptosis-effector gasdermin C genes ( Gsdmc s) as cytokine-regulated target intestinal epithelial cells. Furthermore, we showed that overexpression Gsdmc2 triggered pyroptosis. Organoid cultures vivo mouse models were used to show activating immunity leads lytic cell death gut epithelium...
Wnt and Shh signaling pathways are critical for the development maturation of many epithelial tissues. Both have roles in stem cell maintenance, tissue development, tumorigenesis. However, linkage between these mammalian systems had not been well established. Here, we report that expression fungiform papillae formation normal mature depend on through β-catenin. We observed during papilla mice, components Wnt/β-catenin pathway expressed together developing placode. The elimination either Lef1...
Microglia are highly sensitive to even small changes in the brain environment, such as invasion of non-hazardous toxicants or presymptomatic state diseases. However, physiological pathophysiological consequences their responses remain unknown. Here, we report that cultured microglia sense low concentrations neurotoxicant methylmercury (MeHglow) and provide neuroprotection against MeHg, for which astrocytes also required. When exposed MeHglow, exocytosed ATP via p38 MAPK- vesicular nucleotide...
The distinctive umami taste elicited by l-glutamate and some other amino acids is thought to be initiated G-protein-coupled receptors. Proposed receptors include heteromers of receptor type 1, members 1 3 (T1R1+T1R3), metabotropic glutamate 4 (mGluR1 mGluR4). Multiple lines evidence support the involvement T1R1+T1R3 in responses mice. Although several studies suggest than umami, identity those remains unclear. Here, we examined responsiveness umami-sensitive chorda tympani nerve fibres from...
Leucine-rich repeat-containing G-protein coupled receptor 5-expressing (Lgr5(+)) cells have been identified as stem/progenitor in the circumvallate papillae, and single cultured Lgr5(+) give rise to taste cells. Here we use papilla tissue establish a three-dimensional culture system (taste bud organoids) that develops phenotypic characteristics similar native tissue, including multilayered epithelium containing outer layers inner layers. Furthermore, characterization of cell cycle progenitor...
Key points The taste receptor T1R1 + T1R3 heterodimer and metabotropic glutamate receptors (mGluR) may function as umami receptors. Here, we used mGluR4 knockout (mGluR4‐KO) mice examined the of in peripheral responses mice. mGluR4‐KO showed reduced to l ‐AP4 (mGluR4 agonist) chorda tympani glossopharyngeal nerves without affecting other stimuli. Residual were suppressed by gurmarin (T1R3 blocker) AIDA (group I mGluR antagonist). present study not only provided functional evidence for...
Neuroendocrine cells store ATP in secretory granules and release it along with hormones that may trigger a variety of cellular responses process called purinergic chemical transmission. Although the vesicular nucleotide transporter (VNUT) has been shown to be involved storage ATP, its physiological relevance vivo is far less well understood. In Vnut knockout (Vnut(-/-)) mice, we found loss functional VNUT adrenal chromaffin insulin islets Langerhans led several significant effects. Vesicular...
Intestinal organoids are useful for in vitro investigation of the properties intestinal epithelial cells and their interaction with gut microbiome. Here, we established cecal colonic from common marmosets, which highlighted as model nonhuman primates but susceptible to gastrointestinal diseases. Established were capable passaging long term culture. Results quantitative reverse transcription PCR immunostaining showed differentiation into major cell types epithelium (colonocytes, goblet...
Intestinal organoids are useful for the in vitro investigation of properties intestinal epithelial cells and their interaction with gut microbiome. In this study, we cultured cecal colonic from common marmosets, which highlighted as model nonhuman primates but susceptible to gastrointestinal diseases. The established were capable passaging long-term culture. results quantitative reverse transcription PCR immunostaining showed that differentiated into major cell types (colonocytes, goblet...
Interaction between erythropoietin (EPO) and its membrane receptor induces the proliferation differentiation of erythroid progenitors. EPO has been shown to activate JAK2-STAT5 pathway in various hematopoietic cell lines, although physiological role this is unclear. We have previously that epidermal growth factor activates a chimeric bearing extracellular domain linked cytoplasmic receptor, resulting interleukin-3-dependent cells (globin synthesis) EPO-responsive erythroleukemia cells. In...
Abstract Taste cells undergo constant turnover throughout life; however, the molecular mechanisms governing taste cell generation are not well understood. Using RNA-Seq, we systematically surveyed transcriptome landscape of organoids at different stages growth. Our data show staged expression a variety genes and identify multiple signaling pathways underlying differentiation stem/progenitor proliferation. For example, transcripts receptors appear only or predominantly in late-stage...
In taste cells, receptors, their coupled G proteins and downstream signalling elements mediate the detection transduction of sweet, bitter umami compounds. some intestinal endocrine receptors gustducin contribute to release glucagon-like peptide 1 (GLP-1) other gut hormones in response glucose non-energetic sweeteners. Conversely, cells have been found express multiple typically e.g. GLP-1, glucagon, somatostatin ghrelin. present study, by immunohistochemistry, subsets were GLP-1. The GLP-1...
Abstract The bladder urothelium is more than just a barrier. When the distended, functions as sensor to initiate voiding reflex, during which it releases ATP via multiple mechanisms. However, mechanisms underlying this release in response various stretch stimuli caused by filling remain largely unknown. Therefore, aim of study was elucidate these By comparing vesicular nucleotide transporter (VNUT)-deficient and wild-type male mice, we showed that has crucial role urine storage through...
Intestinal macrophages are largely responsible for the innate immune response and also intestinal homeostasis.1Santaolalla R. et al.Curr Opin Gastroenterol. 2011; 27: 125-131Crossref PubMed Scopus (75) Google Scholar We have developed novel xenogeneic-free human organoids (XF-HIOs) that uniquely structured with an apical-out mucosal epithelium complex mesenchymal tissue, including smooth muscle nerve cells.2Uchida H. al.JCI Insight. 2017; 2e86492Crossref (44) Scholar,3Sasaki K....
Since the discovery of direct chemical synapses between rod photoreceptor and OFF cone bipolar cells in mouse retinas, whether ON cell also receive input from has been a pending question. In finding that metabotropic glutamate receptor 7 (mGluR7) was uniquely expressed dendrites mGluR6-deficient retina, we used this ectopic mGluR7 immunoreactivity as specific marker for to search its connection. Here, show certain type forms ribbon-associated not only with cones, but rods. This verified...
Heterologously expressed sensory receptors generally do not achieve the ligand sensitivity observed in vivo , and may require specific accessory proteins to ensure optimal function. We searched for taste cell-expressed receptor transporting protein (RTP) expression enhancing (REEP) family members that might serve as molecules enhance gustatory determined REEP2 is an integral membrane cells, physically associates with both subunits of type 1 2 3 sweet specifically enhances responses tastants...
Conditioned taste aversion (CTA) is a well-established learning paradigm, whereby animals associate tastes with subsequent visceral illness. The prelimbic cortex (PL) has been shown to be involved in the association of events separated by time. However, nature PL activity and its functional network whole brain during CTA remain unknown. Here, using awake magnetic resonance imaging fiber tracking, we analyzed connectivity blood oxygen level-dependent (BOLD) signal significantly increased...