A5075913583- Biochemical Analysis and Sensing Techniques
- Olfactory and Sensory Function Studies
- Advanced Chemical Sensor Technologies
- Regulation of Appetite and Obesity
- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- RNA and protein synthesis mechanisms
- Neurobiology and Insect Physiology Research
- Diet, Metabolism, and Disease
- FOXO transcription factor regulation
- GABA and Rice Research
- Cholesterol and Lipid Metabolism
- Analytical Chemistry and Chromatography
- Diet and metabolism studies
- Phytochemicals and Antioxidant Activities
- Liver physiology and pathology
- Polyamine Metabolism and Applications
- Postharvest Quality and Shelf Life Management
Kyushu University
2011-2024
Kyushu Dental University
2010-2024
Monell Chemical Senses Center
2019
Tokushima University
2005
Shikoku University
2005
The distinctive umami taste elicited by l-glutamate and some other amino acids is thought to be initiated G-protein-coupled receptors. Proposed receptors include heteromers of receptor type 1, members 1 3 (T1R1+T1R3), metabotropic glutamate 4 (mGluR1 mGluR4). Multiple lines evidence support the involvement T1R1+T1R3 in responses mice. Although several studies suggest than umami, identity those remains unclear. Here, we examined responsiveness umami-sensitive chorda tympani nerve fibres from...
Five fundamental taste qualities (sweet, bitter, salty, sour, umami) are sensed by dedicated cells (TCs) that relay quality information to gustatory nerve fibers. In peripheral signaling pathways, ATP has been identified as a functional neurotransmitter, but it remains be determined how specificity of different is maintained across synapses. Recent studies demonstrated some gut peptides released from buds prolonged application particular stimuli, suggesting their potential involvement in...
The systemic renin-angiotensin system (RAS) is an important regulator of body fluid and sodium homeostasis. Angiotensin II (AngII) a key active product the RAS. We previously revealed that circulating AngII suppresses amiloride-sensitive salt taste responses enhances to sweet compounds via type 1 receptor (AT1) expressed in cells. However, molecular mechanisms underlying modulation function by remain uncharacterized. Here we examined expression three RAS components, namely renin,...
Expression of insulin and its receptor (IR) in rodent taste cells has been proposed, but exactly which types express IR the function signaling organ have yet to be determined. In this study, we analyzed expression mRNA protein mouse bud vivo explored ex organoids, using RT-PCR, immunohistochemistry, quantitative PCR. tissue, was expressed broadly buds, including type II III cells. With 3-D found culture medium significantly decreased number cell levels many genes, nucleoside triphosphate...
Abstract The sweet taste receptor plays an essential role as energy sensor by detecting carbohydrates. However, the dynamic mechanisms of activation remain unclear. Here, we describe interactions between transmembrane domain G protein-coupled subunit, TAS1R3, and allosteric modulators. Molecular dynamics simulations reproduced species-specific sensitivity to ligands. We found that a human-specific sweetener, cyclamate, interacted with mouse negative modulator. Agonist-induced allostery...
Cholecystokinin (CCK) is a gut hormone released from enteroendocrine cells. CCK functions as an anorexigenic factor by acting on receptors expressed the vagal afferent nerve and hypothalamus with synergistic interaction between leptin. In gut, tastants such amino acids bitter compounds stimulate release cells via activation of taste transduction pathways. also in buds, suggesting potential roles signaling peripheral organ. present study, we focused function initial responses to stimulation....
On the tongue, T1R-independent pathway (comprising glucose transporters, including sodium–glucose cotransporter (SGLT1) and KATP channel) detects only sugars, whereas T1R-dependent (T1R2/T1R3) can broadly sense various sweeteners. Cephalic-phase insulin release, a rapid release of induced by sensory signals in head after food-related stimuli, reportedly depends on pathway, competitive sweet taste modulators leptin endocannabinoids may function these two different pathways independently,...
Abstract Taste information is detected by taste cells and then transmitted to the brain through nerve fibers. According our previous data, there may be specific coding of quality between However, molecular mechanisms underlying this specificity remain unclear. The purpose study was identify candidate molecules that regulate coding. GeneChip analysis mRNA isolated from mice papillae ganglia revealed 14 members cadherin superfamily, which are important regulators synapse formation plasticity,...
Taste buds are complex sensory organs embedded in the epithelium of fungiform papillae (FP) and circumvallate (CV). The sweet, bitter, umami tastes sensed by type II taste cells that express receptors (Tas1rs Tas2rs) coupled with G-protein α-gustducin. Recent studies revealed response profiles α-gustducin-expressing different between FP CV, but which genes could generate such distinctive cell characteristics still largely unknown. We performed a comprehensive transcriptome analysis on mouse...
Taste disorders are common adverse effects of cancer chemotherapy that can reduce quality life and impair nutritional status. However, the molecular mechanisms underlying chemotherapy-induced taste remain largely unknown. Furthermore, there no effective preventive measures for disorders. We investigated a combination three anticancer drugs (TPF: docetaxel, cisplatin 5-fluorouracil) on structure function mouse tissues examined whether drinking ice-cold water after TPF administration would...
GPRC5C is an orphan G protein‐coupled receptor (GPCR) that belongs to the class C GPCR family. Although expressed in various organs, its function and ligand are still undetermined. We found mouse taste cells, enterocytes, pancreatic α‐cells. In functional imaging assays, HEK293 cells heterologously expressing chimeric protein α subunit Gα16‐gust44 showed robust intracellular Ca 2+ increases response monosaccharides, disaccharides, a sugar alcohol, but not artificial sweetener or...
窒素ガス加圧によるスダチ果汁中の溶存酸素の除去について検討を行い, 以下の結果を得た.(1) スダチ果汁を窒素ガスで直接加圧すると, 加圧時間を長くすることで, スダチ果汁中の溶存酸素はより減少し, 圧力を上げることで, より多くの溶存酸素が除去できた.(2) スダチ果汁に対して, 窒素ガス加圧と除圧を繰り返すと, スダチ果汁中の溶存酸素は短時間で減少し, 30℃, 10MPaで30秒間加圧した後, 常圧に戻す処理を, 3回繰り返したところ, 溶存酸素量は1mg/l以下まで減少した. さらに, 処理回数を増やすごとに溶存酸素量は減少し, 20秒間を7回 (計2分20秒間) の処理では0.02mg/lと, 非常に低い濃度まで溶存酸素を除去することができた.(3) スダチ果汁の香りに及ぼす窒素ガス加圧の影響を官能検査で調べた結果, 10MPaで1分間以下の条件 (20秒間×3, 30秒間×2, 1分間×1) では, 香りを保持したままスダチ果汁中の溶存酸素を除去することができた.(4) スダチ果汁の窒素ガス加圧処理は, スダチ果汁保存中の酸化を防止し,...
Little is known about the molecular mechanisms underlying drug-induced taste disorders, which can cause malnutrition and reduce quality of life. One disorders adverse effects bisphosphonates, are administered as anti-osteoporotic drugs. Therefore, present study evaluated risedronate (a bisphosphonate) on bud cells. Expression analyses revealed that farnesyl diphosphate synthase (FDPS, a key enzyme in mevalonate pathway) was subset mouse tongue epithelial cells, especially type III...
味覚は食欲や摂食内容と関連する重要な感覚である. これまで, 味覚障害患者を対象とした味覚試験は多数報告されているが, 健常者を対象としたものは少ない. われわれは, 一般地域住民を対象とした大規模疫学調査である岩木健康増進プロジェクトの2019年度の参加者を対象に, 全口腔法により甘味, 塩味, 酸味, 苦味の閾値を調査し, データの得られた1,027名について解析を行った.
Abstract Drug-induced taste disorders reduce quality of life, but little is known about the molecular mechanisms by which drugs induce disturbances. In this study, we investigated short-term and long-term effects antiarrhythmic drug flecainide, to cause dysfunction. Analyses behavioral responses (licking tests) revealed that mice given a single intraperitoneal injection flecainide exhibited significant reduction in preference for sour tastant (HCl) not other solutions (NaCl, quinine,...