A5054472689- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Cancer-related Molecular Pathways
- Cancer-related gene regulation
- Retinoids in leukemia and cellular processes
- Animal Genetics and Reproduction
- Immune Cell Function and Interaction
- RNA Research and Splicing
- Telomeres, Telomerase, and Senescence
- Protein Degradation and Inhibitors
- Cytokine Signaling Pathways and Interactions
- Cancer Cells and Metastasis
- Histone Deacetylase Inhibitors Research
- Signaling Pathways in Disease
- Viral Infectious Diseases and Gene Expression in Insects
- Ubiquitin and proteasome pathways
- Genomics, phytochemicals, and oxidative stress
- IL-33, ST2, and ILC Pathways
- Pancreatic function and diabetes
- Renal and related cancers
- RNA modifications and cancer
Kaohsiung Medical University
2015-2025
Kaohsiung Medical University Chung-Ho Memorial Hospital
2012-2025
Taipei Institute of Pathology
2024
Waseda University
2020-2022
The University of Tokyo
2010-2021
Tokushima Bunri University
2015-2017
University of Tsukuba
2017
Rutgers, The State University of New Jersey
2015
RIKEN BioResource Research Center
2001-2010
RIKEN
2000-2010
Src-family tyrosine kinases (SFKs), which participate in a variety of signal transduction events, are known to localize the cytoplasmic face plasma membrane through lipid modification. Recently, we showed that Lyn, an SFK member, is exocytosed via Golgi region along secretory pathway. We show here trafficking specified by palmitoylation state. Yes also monopalmitoylated and biosynthetically transported from pool caveolin membrane. This pathway can be inhibited trans-Golgi network...
Embryonic stem (ES) cells are pluripotent with the potential capacity to generate any type of cell. We describe here isolation ES‐like from equine blastocysts that have been frozen and thawed. Our two lines (E‐1 E‐2) appear maintain a normal diploid karyotype indefinitely in culture vitro express markers characteristic ES mice, namely, alkaline phosphatase, stage‐specific embryonic antigen‐1, STAT‐3 Oct 4. After for more than 17 passages, some differentiated neural precursor presence basic...
The cAMP-response element-binding protein-binding protein (CBP) and p300 are common coactivators for several transcriptional factors. It has been reported that both CBP significant the activation of peroxisome proliferator-activated receptor γ (PPARγ), which is a crucial nuclear in adipogenesis. However, it remains unclear whether and/or physiologically essential to PPARγ adipocytes adipocyte differentiation. In this study, we investigated physiological significance CBP/p300 NIH3T3 cells...
Mechanical strain has been implicated in phenotypic changes, including alteration of gene expression vascular smooth muscle cells; however, the molecular basis for mechanotransduction leading to nuclear is largely unknown. We demonstrate present study that cyclic stretching cells dramatically activates Jun N-terminal kinase (JNK)/stress-activated protein (SAPK) through an autocrine mechanism. Stretch causes time- and strength-dependent rise ATP concentration media. The stretch-induced...
The expression of CLC-K1 and CLC-K2, two kidney-specific CLC chloride channels, is transcriptionally regulated on a tissue-specific basis. Previous studies have shown that GA element near their transcriptional start sites important for basal cell-specific activities the CLC-K2 gene promoters. To identify GA-binding proteins, human kidney cDNA library was screened by yeast one-hybrid system. A novel member Cys2-His2 zinc finger designated KKLF (for "kidney-enriched Krüppel-like factor")...
DNA fragmentation is one of the critical steps in apoptosis, which induced by factor (DFF). DFF composed two subunits, a 40 kDa caspase-activated nuclease (DFF40) and 45 inhibitor (DFF45). Recently novel family cell-death-inducing DFF45-like effectors (CIDEs) has been identified. Among CIDEs, from human (CIDE-A CIDE-B) three mouse (CIDE-A, CIDE-B FSP27) have reported. In this study CIDE-3, member was identified upon sequence analysis previously unidentified cDNA that encoded protein 238...
Rat pheochromocytoma PC12 cells undergo neuronal differentiation in response to nerve growth factor (NGF). The involves protein kinase cascades that include the kinases MEK and ERK, as well activation of transcription factors c‐Jun c‐Fos. We show here, exposure mannosylerythritol lipid (MEL), a yeast extracellular glycolipid, enhances activity acetylcholinesterase interrupts cell cycle at G1 phase, with resulting outgrowth neurites partial cellular differentiation. Treatment MEL stimulates...
Up-regulation of the c-jun gene is a critical event in retinoic acid (RA)-mediated differentiation embryonal carcinoma F9 cells. Activating transcription factor 2 (ATF-2) and p300 cooperate activation during We show here that overexpression Jun dimerization protein (JDP2), repressor AP-1, inhibits transactivation by ATF-2 recruitment histone deacetylase 3 (HDAC3) complex, thereby repressing RA-induced inhibiting RA-mediated Moreover, chromatin immunoprecipitation assays showed JDP2/HDAC3...
The Sp family is a of transcription factors that bind to cis ‐elements in the promoter regions various genes. Regulation by proteins based on interactions between GC‐rich binding site (GGGCGG) DNA and C‐terminal zinc finger motifs proteins. In this study, we characterized gene for methyltransferase ( Dnmt 1) responsible methylation cytosine residues mammals plays role silencing. We found ‐element (nucleotides −161 −147) was essential expression mouse 1. DNA‐binding assays indicated Sp1 Sp3...
Studies have demonstrated that the retinoblastoma susceptibility gene product, RB, can either positively or negatively regulate expression of several genes through cis-acting elements in a cell-type-dependent manner. The nucleotide sequence control element (RCE) motif, GCCACC CCACCC, and Sp1 consensus binding sequence, CCGCCC, confer equal responsiveness to RB. Here, we report RB activates transcription c-jun Sp1-binding site within promoter. Preincubation crude nuclear extracts with...
Premenopausal women have better survival than men after intracerebral hemorrhage, which is associated with iron overproduction and autophagy induction. To examine the participation of neuronal estrogen receptor α (ERα) in E 2-mediated protection, PC12 neurons treated Atg7 (autophagy-related protein 7) siRNA, rapamycin (an inducer), or Erα siRNA were applied. study whether involves β-estradiol 3-benzoate (E 2)-mediated neuroprotection against iron-induced striatal injury, castration 2 capsule...
BRCA1-associated breast cancers are associated with particular features such as early onset, poor histological differentiation, and hormone receptor negativity. Previous studies conducted in Taiwanese population showed that the mutation of BRCA1 gene does not play a significant role occurrence cancer. The present study explored methylation promoter its relationship to clinical outcome cancer patients. Tumor specimens from cohort 139 early-stage patients were obtained during surgery before...
Abstract Oxidative stress and reactive oxygen species (ROS) are associated with diseases such as cancer, cardiovascular complications, inflammation neurodegeneration. Cellular defense systems must work constantly to control ROS levels prevent their accumulation. We report here that the Jun dimerization protein 2 (JDP2) has a critical role cofactor for transcription factors nuclear factor-erythroid 2-related factor (Nrf2) small Maf family K (MafK) in regulation of antioxidant-responsive...
Abstract The androgen receptor (AR) has a critical role in promoting androgen-dependent and -independent apoptosis testicular cells. However, the molecular mechanisms that underlie ligand-independent apoptosis, including activity of AR stem cells, are not completely understood. In present study, we generated induced pluripotent cells (iPSCs) from bovine by electroporation octamer-binding transcription factor 4 ( OCT4 ). were supplemented with leukemia inhibitory bone morphogenetic protein 4,...
The network of stemness genes and oncogenes in human patient-specific reprogrammed cancer stem cells (CSCs) remains elusive, especially liver cancer. HepG2-derived induced pluripotent cell-like (HepG2-iPS-like cells) were generated by introducing Yamanaka factors the knockdown vector shTP53. They exhibited features a higher tumorigenesis after xenograft transplantation compared with HepG2 cells. cancerous mass severe combined immunodeficiency (SCID) mice derived from one colony was dissected...