A5065981856- RNA and protein synthesis mechanisms
- Adipose Tissue and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Fatty Acid Research and Health
- RNA modifications and cancer
- Pancreatic function and diabetes
- Peptidase Inhibition and Analysis
- Lipid metabolism and biosynthesis
- Neuropeptides and Animal Physiology
- Peroxisome Proliferator-Activated Receptors
- RNA Research and Splicing
- Genomics and Phylogenetic Studies
- Diet and metabolism studies
- Advanced Proteomics Techniques and Applications
- Metabolism, Diabetes, and Cancer
- Receptor Mechanisms and Signaling
- Cholesterol and Lipid Metabolism
- Mitochondrial Function and Pathology
- Microbial Metabolic Engineering and Bioproduction
- Chemical Synthesis and Analysis
- Click Chemistry and Applications
- CRISPR and Genetic Engineering
- Machine Learning in Bioinformatics
- Cannabis and Cannabinoid Research
- Lipid Membrane Structure and Behavior
Salk Institute for Biological Studies
2016-2025
College of the Atlantic
2024
Clayton Foundation
2019
La Jolla Alcohol Research
2018
Harvard University
2007-2016
Harvard University Press
2007-2015
University of Pennsylvania
2013
Brigham and Women's Hospital
2013
IIT@Harvard
2012
Scripps Research Institute
2001-2008
It has been assumed that most, if not all, signals regulating early development have identified. Contrary to this expectation, we identified 28 candidate signaling proteins expressed during zebrafish embryogenesis, including Toddler, a short, conserved, and secreted peptide. Both absence overproduction of Toddler reduce the movement mesendodermal cells gastrulation. Local ubiquitous production promote cell movement, suggesting is neither an attractant nor repellent but acts globally as...
Metalloproteases (MPs) are a large and diverse class of enzymes implicated in numerous physiological pathological processes, including tissue remodeling, peptide hormone processing, cancer. MPs tightly regulated by multiple posttranslational mechanisms vivo , hindering their functional analysis conventional genomic proteomic methods. Here we describe general strategy for creating activity-based probes coupling zinc-chelating hydroxamate to benzophenone photocrosslinker, which promote...
Abstract Antibiotic-induced microbiome depletion (AIMD) has been used frequently to study the role of gut in pathological conditions. However, unlike germ-free mice, effects AIMD on host metabolism remain incompletely understood. Here we show elucidate its homeostasis, luminal signaling, and metabolism. We demonstrate that AIMD, which decreases Firmicutes Bacteroidetes species, baseline serum glucose levels, reduces surge a tolerance test, improves insulin sensitivity without altering...
Significance Metastasis is a major cause of cancer mortality. However, the regulation this complex process remains poorly understood. Due to low oxygen supply and enhanced sugar metabolism, cells releases lactate create an acidic environment. We show that membrane receptor on macrophages called G protein-coupled 132 (Gpr132) can sense respond signal from cells. As result, alter their functions, which, in turn, stimulates metastasis distant organs. Consequently, loss Gpr132 mice inhibits...
N-Acyl ethanolamines (NAEs) constitute a large and diverse class of signaling lipids that includes the endogenous cannabinoid anandamide. Like other lipid transmitters, NAEs are thought to be biosynthesized degraded on-demand rather than being stored in vesicles prior signaling. The identification enzymes involved NAE metabolism is therefore imperative achieve complete understanding this system control it for potential therapeutic gain. Recently, an N-acyl phosphatidylethanolamine...
The AMP-activated protein kinase (AMPK) is a highly conserved master regulator of metabolism, whose activation has been proposed to be therapeutically beneficial for the treatment several metabolic diseases, including nonalcoholic fatty liver disease (NAFLD). NAFLD, characterized by excessive accumulation hepatic lipids, most common chronic and major risk factor development steatohepatitis, type 2 diabetes, other conditions. To assess therapeutic potential AMPK activation, we have generated...