A5025567563- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Zebrafish Biomedical Research Applications
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Genomics and Chromatin Dynamics
- Retinal Development and Disorders
- Epigenetics and DNA Methylation
- Blood Coagulation and Thrombosis Mechanisms
- Platelet Disorders and Treatments
- Neuroblastoma Research and Treatments
- Photoreceptor and optogenetics research
- Genetics and Neurodevelopmental Disorders
- Pluripotent Stem Cells Research
- Chromosomal and Genetic Variations
- Genetic Neurodegenerative Diseases
- Mitochondrial Function and Pathology
- Innovation and Socioeconomic Development
- Plant tissue culture and regeneration
- RNA regulation and disease
- Genetics, Aging, and Longevity in Model Organisms
- Nicotinic Acetylcholine Receptors Study
- DNA Repair Mechanisms
- Myeloproliferative Neoplasms: Diagnosis and Treatment
Massachusetts General Hospital
2012-2023
Harvard University
2011-2023
Center for Cancer Research
2011-2023
Editas Medicine (United States)
2017-2021
Biogen (United States)
2017
Boston University
2017
Laboratoire d’immunologie intégrative du cancer
2017
Iowa State University
2009-2013
Howard Hughes Medical Institute
2013
Harvard University Press
2012
It has been assumed that most, if not all, signals regulating early development have identified. Contrary to this expectation, we identified 28 candidate signaling proteins expressed during zebrafish embryogenesis, including Toddler, a short, conserved, and secreted peptide. Both absence overproduction of Toddler reduce the movement mesendodermal cells gastrulation. Local ubiquitous production promote cell movement, suggesting is neither an attractant nor repellent but acts globally as...
ZiFiT (Zinc Finger Targeter) is a simple and intuitive web-based tool that provides an interface to identify potential binding sites for engineered zinc finger proteins (ZFPs) in user-supplied DNA sequences. In this updated version, identifies ZFPs made by both the modular assembly OPEN engineering methods. addition, now integrates additional tools resources including scoring schemes assembly, with Zinc Database (ZiFDB) of ZFPs, direct querying NCBI BLAST servers identifying off-target...
We have previously reported a simple and customizable CRISPR (clustered regularly interspaced short palindromic repeats) RNA-guided Cas9 nuclease (RGN) system that can be used to efficiently robustly introduce somatic indel mutations in endogenous zebrafish genes. Here we demonstrate RGN-induced are heritable, with efficiencies of germline transmission reaching as high 100%. In addition, extend the power RGN by showing these nucleases single-stranded oligodeoxynucleotides (ssODNs) create...
We report here an efficient method for targeted mutagenesis of Arabidopsis genes through regulated expression zinc finger nucleases (ZFNs)—enzymes engineered to create DNA double-strand breaks at specific target loci. ZFNs recognizing the ADH1 and TT4 were made by Oligomerized Pool ENgineering (OPEN)—a publicly available, selection-based platform that yields high quality arrays. The placed under control estrogen-inducible promoter introduced into plants floral-dip transformation. Primary...
We performed targeted mutagenesis of a transgene and nine endogenous soybean (Glycine max) genes using zinc-finger nucleases (ZFNs). A suite ZFNs were engineered by the recently described context-dependent assembly platform--a rapid, open-source method for generating arrays. Specific targeting dicer-like (DCL) other involved in RNA silencing cloned into vector under an estrogen-inducible promoter. hairy-root transformation system was employed to investigate efficiency ZFN at each target...
Transcription activator-like effector nucleases (TALENs) are powerful new research tools that enable targeted gene disruption in a wide variety of model organisms.Recent work has shown TALENs can induce mutations endogenous zebrafish genes, but to date only four genes have been altered, and larger-scale tests the success rate, mutation efficiencies germline transmission rates not described.Here, we constructed homodimeric 10 different targets various found 7 nuclease pairs induced indel with...
Background Customized zinc finger nucleases (ZFNs) form the basis of a broadly applicable tool for highly efficient genome modification. ZFNs are artificial restriction endonucleases consisting non-specific nuclease domain fused to array which can be engineered recognize specific DNA sequences interest. Recent proof-of-principle experiments have shown that targeted knockout mutations efficiently generated in endogenous zebrafish genes via non-homologous end-joining-mediated repair...
Zinc Finger Nucleases (ZFNs) made by Context-Dependent Assembly (CoDA) and Transcription Activator-Like Effector (TALENs) provide robust user-friendly technologies for efficiently inactivating genes in zebrafish. These designer nucleases bind to cleave DNA at particular target sites, inducing error-prone repair that can result insertion or deletion mutations. Here, we assess the relative efficiencies of these somatic mutations mosaic We find TALENs exhibited a higher success rate obtaining...
CRISPR/Cas9 is an attractive platform to potentially correct dominant genetic diseases by gene editing with unprecedented precision. In the current proof-of-principle study, we explored use of for gene-editing in myotonic dystrophy type-1 (DM1), autosomal-dominant muscle disorder, excising CTG-repeat expansion 3′-untranslated-region (UTR) human protein kinase (DMPK) DM1 patient-specific induced pluripotent stem cells (DM1-iPSC), DM1-iPSC-derived myogenic and myoblasts. To eliminate...
BRCA1 is a breast and ovarian tumor suppressor. Given its numerous incompletely understood functions the possibility that more exist, we performed complementary systematic screens in search of new protein-interacting partners. New and/or better understanding existing ones were sought. Among interacting proteins identified, genetic interactions detected between four interactors: TONSL, SETX, TCEANC, TCEA2. Genetic also certain interactors including both members FACT complex. From these...