A5101652056- Pharmacological Receptor Mechanisms and Effects
- Advanced X-ray and CT Imaging
- Nicotinic Acetylcholine Receptors Study
- Radiation Dose and Imaging
- Geotechnical Engineering and Underground Structures
- Fluid Dynamics Simulations and Interactions
- CRISPR and Genetic Engineering
- Numerical methods in engineering
- Receptor Mechanisms and Signaling
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
Harvard University
2016
Massachusetts General Hospital
2016
Harvard University Press
2012
Transcription activator-like effector nucleases (TALENs) are powerful new research tools that enable targeted gene disruption in a wide variety of model organisms.Recent work has shown TALENs can induce mutations endogenous zebrafish genes, but to date only four genes have been altered, and larger-scale tests the success rate, mutation efficiencies germline transmission rates not described.Here, we constructed homodimeric 10 different targets various found 7 nuclease pairs induced indel with...
We simulate the formation of spontaneous ruptures in supported phospholipid double bilayer membranes, using peridynamic modeling. Experiments performed on spreading bilayers typically show two distinct kinds ruptures, floral and fractal, which form spontaneously distal (upper) at late stages high energy substrates. It is, however, currently unresolved factors govern occurrence either rupture type. Variations distance between bilayers, interconnections ("pinning sites") are suspected...