A5075656434- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Cellular Mechanics and Interactions
- Zebrafish Biomedical Research Applications
- Retinal Development and Disorders
- Microtubule and mitosis dynamics
- Dermatology and Skin Diseases
- Autoimmune Bullous Skin Diseases
- Medicine and Dermatology Studies History
- Urticaria and Related Conditions
- Neurobiology and Insect Physiology Research
- Pluripotent Stem Cells Research
- Advanced Electron Microscopy Techniques and Applications
- Effects of Radiation Exposure
- Image Processing Techniques and Applications
- Health and Medical Studies
- Developmental Biology and Gene Regulation
- Photoreceptor and optogenetics research
- Neural dynamics and brain function
- Medical and Health Sciences Research
- Congenital heart defects research
- 3D Printing in Biomedical Research
- Genomics and Chromatin Dynamics
- Molecular Biology Techniques and Applications
Howard Hughes Medical Institute
2014-2024
Janelia Research Campus
2015-2024
Helix (United States)
2010-2021
Zurich University of Applied Sciences in Business Administration
2020
University Hospital of Zurich
2011-2017
European Molecular Biology Organization
2008-2015
European Molecular Biology Laboratory
2007-2012
Max Planck Institute of Molecular Cell Biology and Genetics
2012
ETH Zurich
2012
European Molecular Biology Laboratory
2005-2010
A long-standing goal of biology is to map the behavior all cells during vertebrate embryogenesis. We developed digital scanned laser light sheet fluorescence microscopy and recorded nuclei localization movement in entire wild-type mutant zebrafish embryos over first 24 hours development. Multiview vivo imaging at 1.5 billion voxels per minute provides “digital embryos,” that is, comprehensive databases cell positions, divisions, migratory tracks. Our analysis global division patterns reveals...
Significance Many chemical and biomedical techniques rely on slow diffusive transport because existing pressure-based methods or electrokinetic can incidentally damage the sample. This study introduces a novel concept termed stochastic electrotransport that selectively nondestructively expedite of electromobile molecules into porous sample, such as fixed biological tissues. We use method to rapidly several classes whole mouse brains other organs achieve rapid clearing staining entire tissue...
Abstract Understanding how the brain works in tight concert with rest of central nervous system (CNS) hinges upon knowledge coordinated activity patterns across whole CNS. We present a method for measuring an entire, non-transparent CNS high spatiotemporal resolution. combine light-sheet microscope capable simultaneous multi-view imaging at volumetric speeds 25-fold faster than state-of-the-art, whole-CNS assay isolated Drosophila larval and computational framework analysing multi-view,...
During development, coordinated cell behaviors orchestrate tissue and organ morphogenesis. Detailed descriptions of lineages provide a powerful framework to elucidate the mechanisms To study cellular basis limb we imaged transgenic fluorescently-labeled embryos from crustacean Parhyale hawaiensis with multi-view light-sheet microscopy at high spatiotemporal resolution over several days embryogenesis. The lineage outgrowing thoracic limbs was reconstructed single-cell new software called...
The mammalian heart is derived from multiple cell lineages; however, our understanding of when and how the diverse cardiac types arise limited. We mapped origin embryonic mouse at single-cell resolution using a combination transcriptomic, imaging, genetic lineage labeling approaches. This mapping provided transcriptional anatomic definition progenitor types. Furthermore, it revealed pool that anatomically transcriptionally distinct currently known progenitors. Besides contributing to...