A5085712951- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Advanced Electron Microscopy Techniques and Applications
- CRISPR and Genetic Engineering
- Photoacoustic and Ultrasonic Imaging
- Marine Ecology and Invasive Species
- Functional Brain Connectivity Studies
- Circadian rhythm and melatonin
- Neuroscience and Neuropharmacology Research
- Protist diversity and phylogeny
- Virus-based gene therapy research
- Bacterial Genetics and Biotechnology
- Receptor Mechanisms and Signaling
- Viral gastroenteritis research and epidemiology
- Congenital heart defects research
- Morphological variations and asymmetry
- Developmental Biology and Gene Regulation
- Bacterial biofilms and quorum sensing
- Treatment of Major Depression
- Marine Invertebrate Physiology and Ecology
- Regulation of Appetite and Obesity
- Echinoderm biology and ecology
- Bacteriophages and microbial interactions
- Neural dynamics and brain function
Columbia University
2017-2025
Allen Institute for Brain Science
2023
Jackson Laboratory
2023
University of California, San Diego
2023
Brain (Germany)
2023
Columbia University Irving Medical Center
2019
Howard Hughes Medical Institute
2011-2017
Stanford University
2014-2017
Helix (United States)
2011-2014
Janelia Research Campus
2011-2014
Planktonic ciliated larvae are characteristic for the life cycle of marine invertebrates. Their most prominent feature is apical organ harboring sensory cells and neurons largely undetermined function. An elucidation relationships between various forms primary organs key to understanding evolution animal cycles. These have remained enigmatic due scarcity comparative molecular data. To compare larval body patterning, we studied regionalization episphere, upper hemisphere trochophore larva...
Cells must access resources to survive, and the anatomy of multicellular structures influences this access. In diverse eukaryotes, are provided by internal conduits that allow substances travel more readily through tissue than they would via diffusion. Microbes growing in structures, called biofilms, also affected differential we hypothesized is influenced physical arrangement cells. study, examined microanatomy biofilms formed pathogenic bacterium Pseudomonas aeruginosa discovered clonal...
Advances in tissue clearing and molecular labeling methods are enabling unprecedented optical access to large intact biological systems. These developments fuel the need for high-speed microscopy approaches image samples quantitatively at high resolution. While light sheet (LSM), with its planar imaging speed low photo-bleaching, can be effective, scaling up larger volumes has been hindered by use of orthogonal illumination. To address this fundamental limitation, we have developed theta...
Abstract We utilized forebrain organoids generated from induced pluripotent stem cells of patients with a syndromic form Autism Spectrum Disorder (ASD) homozygous protein-truncating mutation in CNTNAP2 , to study its effects on embryonic cortical development. Patients this present clinical characteristics brain overgrowth. Patient-derived displayed an increase volume and total cell number that is driven by increased neural progenitor proliferation. Single-cell RNA sequencing revealed...
Abstract Optimal distribution of heterogeneous organelles and cell types within an organ is essential for physiological processes. Unique the ovary, hormonally regulated folliculogenesis, ovulation, luteal formation/regression associated vasculature changes lead to tissue remodeling during each reproductive cycle. Using CLARITY approach marker immunostaining, we identified individual follicles corpora lutea in intact ovaries. Monitoring lifetime follicle populations showed age-dependent...
The origin of chordates has been debated for more than a century, with one key issue being the emergence notochord. In vertebrates, notochord develops by convergence and extension chordamesoderm, population midline cells unique molecular identity. We identify mesodermal in developing invertebrate, marine annelid Platynereis dumerilii, that converges extends toward expresses notochord-specific combination genes. These differentiate into longitudinal muscle, axochord, is positioned between...
Gene editing in the brain has been challenging because of restricted transport imposed by blood–brain barrier (BBB). Current approaches mainly rely on local injection to bypass BBB. However, such administration is highly invasive and not amenable treating certain delicate regions brain. We demonstrate a safe effective gene technique using focused ultrasound (FUS) transiently open BBB for intravenously delivered CRISPR/Cas9 machinery
Neuronal calcium (Ca(2+))-binding proteins 1 and 2 (NECAB1/2) are members of the phylogenetically conserved EF-hand Ca(2+)-binding protein superfamily. To date, NECABs have been explored only to a limited extent and, so far, not at all spinal level. Here, we describe distribution, phenotype, nerve injury-induced regulation NECAB1/NECAB2 in mouse dorsal root ganglia (DRGs) cord. In DRGs, NECAB1/2 expressed around 70% mainly small- medium-sized neurons. Many colocalize with calcitonin...
Ketamine is a multifunctional drug with clinical applications as an anesthetic, pain management medication, and fast-acting antidepressant. However, it also recreationally abused for its dissociative effects. Recent studies in rodents are revealing the neuronal mechanisms mediating actions, but impact of prolonged exposure to ketamine on brain-wide networks remains less understood. Here, we develop sub-cellular resolution whole-brain phenotyping approach utilize male mice show that repeated...
Significance The interplay between the anorexigenic and orexigenic neurons in arcuate nucleus that contributes to control of feeding remains elusive. Using optogenetic stimulation, we show activation POMC rapidly inhibits behavior fasted animals. However, simultaneous stimulation both a subset express AgRP is sufficient reverse inhibition trigger intense behavior. We used 3D imaging functional studies illuminate anatomical underpinning inhibitory excitatory events. Our work suggests...
Abstract Among contributors to diffusible signaling are portal systems which join two capillary beds through connecting veins. Portal allow signals be transported in high concentrations directly from one bed the other without dilution systemic circulation. Two have been identified brain. The first was discovered almost a century ago and connects median eminence anterior pituitary gland. second few years links suprachiasmatic nucleus organum vasculosum of lamina terminalis, sensory...
Abstract Advances in 3D neuronal cultures, such as brain spheroids and organoids, are allowing unprecedented vitro access to some of the molecular, cellular developmental mechanisms underlying diseases. However, their efficacy recapitulating network properties that encode function remains limited, thereby precluding development effective models complex disorders like schizophrenia. Here, we develop characterize a Modular Neuronal Network (MoNNet) approach recapitulates specific features...