A5011807319- Bacteriophages and microbial interactions
- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Microbial infections and disease research
- Protein Kinase Regulation and GTPase Signaling
- Melanoma and MAPK Pathways
- Viral Infections and Immunology Research
- Microbial Fuel Cells and Bioremediation
- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
- Viral Infections and Vectors
- PI3K/AKT/mTOR signaling in cancer
- Plant nutrient uptake and metabolism
- Metabolism, Diabetes, and Cancer
- Cancer-related Molecular Pathways
- Pancreatic function and diabetes
- Hippo pathway signaling and YAP/TAZ
- Plant and Fungal Interactions Research
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Virus-based gene therapy research
University of California, San Diego
2023-2025
Columbia University
2017-2024
National Institutes of Health
2016-2020
Cells must access resources to survive, and the anatomy of multicellular structures influences this access. In diverse eukaryotes, are provided by internal conduits that allow substances travel more readily through tissue than they would via diffusion. Microbes growing in structures, called biofilms, also affected differential we hypothesized is influenced physical arrangement cells. study, examined microanatomy biofilms formed pathogenic bacterium Pseudomonas aeruginosa discovered clonal...
Diverse organisms secrete redox-active antibiotics, which can be used as extracellular electron shuttles by resistant microbes. Shuttle-mediated metabolism support survival when substrates are available not locally but rather at a distance. Such conditions arise in multicellular communities, where the formation of chemical gradients leads to resource limitation for cells depth. In pathogenic bacterium Pseudomonas aeruginosa PA14, antibiotics called phenazines act oxidants balance...
Targeting proteins to specific subcellular destinations is essential in prokaryotes, eukaryotes, and the viruses that infect them. Chimalliviridae phages encapsulate their genomes a nucleus-like replication compartment composed of protein chimallin (ChmA) excludes ribosomes decouples transcription from translation. These selectively partition between phage nucleus bacterial cytoplasm. Currently, genes signals govern selective import into are unknown. Here, we identify two components this...
Many eukaryotic viruses require membrane-bound compartments for replication, but no such organelles are known to be formed by prokaryotic
Introns containing homing endonucleases are widespread in nature and have long been assumed to be selfish elements that provide no benefit the host organism. These genetic common viruses, but whether they confer a selective advantage is unclear. In this work, we studied intron-encoded endonuclease gp210 bacteriophage ΦPA3 found it contributes viral competition by interfering with replication of coinfecting phage, ΦKZ. We show targets specific sequence ΦKZ, which prevents assembly progeny...
Bacteriophages must hijack the gene expression machinery of their bacterial host to efficiently replicate. Recently, we have shown that early-expressed protein gp014 Pseudomonas nucleus-forming phage phiKZ forms a stable complex with ribosomes and modulates overall profile during infection. Here, discover phage, designated Churi, is closely related phiKZ. Churi encodes gp335, homolog gp014-phiKZ, which expressed early stages infection, its overexpression in cells interferes growth,...
During development, the Hippo signaling pathway regulates key physiological processes, such as control of organ size, regeneration, and stem cell biology. Yes-associated protein (YAP) is a major transcriptional co-activator pathway. The scaffold IQGAP1 interacts with more than 100 binding partners to integrate diverse pathways. In this study, we report that binds YAP modulates its activity. co-immunoprecipitated from cells. vitro analysis pure proteins demonstrated direct interaction between...
Antibiotic-resistant bacteria present an emerging challenge to human health. Their prevalence has been increasing across the globe due in part liberal use of antibiotics that pressured them develop resistance. Those acquire mobile genetic elements are especially concerning because those plasmids may be shared readily with other microbes can then also become antibiotic resistant. Serious infections have recently related contamination preservative-free eyedrops extensively drug-resistant (XDR)...
Abstract Mobile introns containing homing endonucleases are widespread in nature and have long been assumed to be selfish elements that provide no benefit the host organism. These genetic common viruses, but whether they confer a selective advantage is unclear. Here we studied mobile intron bacteriophage ΦPA3 found its endonuclease gp210 contributes viral competition by interfering with virogenesis of co-infecting phage ΦKZ. We show targets specific sequence competitor ΦKZ, preventing...
Sectioning via paraffin embedding is a broadly established technique in eukaryotic systems. Here we provide method for the fixation, embedding, and sectioning of intact microbial colony biofilms using perfused wax. To adapt this use on biofilms, developed techniques maintaining each sample its growth substrate laminating it with an agar overlayer, added lysine to fixative solution. These optimizations improve retention preservation micromorphological features. Samples prepared manner are...
Abstract The Ras family of small GTPases modulates numerous essential processes. Activating mutations result in hyper-activation selected signaling cascades, which leads to human diseases. high frequency malignant neoplasms has led being a desirable chemotherapeutic target. IQGAP scaffold proteins binds and regulates multiple molecules, including the Rho Rac1 Cdc42. There are conflicting data published literature regarding interactions between proteins. Initial reports showed no binding, but...
AMP-activated protein kinase (AMPK) is a fundamental component of cascade that an energy sensor. AMPK maintains homeostasis in the cell by promoting catabolic and inhibiting anabolic pathways. Activation requires phosphorylation liver B1 or Ca2+/calmodulin-dependent 2 (CaMKK2). The scaffold IQGAP1 regulates intracellular signaling pathways, such as mitogen-activated AKT cascades. Recent work implicates participation metabolic function, but molecular mechanisms underlying these effects are...
Antibacterial proteins inhibiting
ABSTRACT Cells must access resources to survive, and the anatomy of multicellular structures influences this access. In diverse eukaryotes, are provided by internal conduits that allow substances travel more readily through tissue than they would via diffusion. Microbes growing in structures, called biofilms, also affected differential we hypothesized is influenced physical arrangement cells. study, examined microanatomy biofilms formed pathogenic bacterium Pseudomonas aeruginosa discovered...
Sectioning via paraffin embedding is a broadly established technique in eukaryotic systems. Here we provide method for the fixation, embedding, and sectioning of intact microbial colony biofilms using perfused wax. To adapt this use on biofilms, developed techniques maintaining each sample its growth substrate laminating it with an agar overlayer, added lysine to fixative solution. These optimizations improve retention preservation micromorphological features. Samples prepared manner are...
ABSTRACT Antibiotic resistant bacteria present an emerging challenge to human health as the pressure instituted on microbial world through liberal use of antibiotics has resulted in their emergence across globe. Those that acquire mobile genetic elements such plasmids are especially concerning because those may be shared readily with other microbes then can also become antibiotic resistant. Serious infections have recently been related contamination preservative-free eyedrops extensively...
Targeting proteins to specific subcellular destinations is essential in prokaryotes, eukaryotes, and the viruses that infect them. Chimalliviridae phages encapsulate their genomes a nucleus-like replication compartment composed of protein chimallin (ChmA) excludes ribosomes decouples transcription from translation. These selectively partition between phage nucleus bacterial cytoplasm. Currently, genes signals govern selective import into are unknown. Here we identify two components this...
The Ras family of small GTPases modulates numerous essential processes, including development and tissue repair. Activating mutations occur in approximately 30% human malignancies understanding the molecules that modulate function is key to elucidating its role oncogenesis. IQGAP scaffold proteins regulates signaling proteins, members MAPK pathway Rho RAC1 CDC42. However, it controversial whether interact with GTPases. While initial reports showed no binding between IQGAP1 ( EMBO J 1996;...