A5032347914- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Microbial Community Ecology and Physiology
- Innovative Microfluidic and Catalytic Techniques Innovation
- Antifungal resistance and susceptibility
- Cystic Fibrosis Research Advances
- Microbial Fuel Cells and Bioremediation
- Hemoglobin structure and function
- Photoreceptor and optogenetics research
- Intracerebral and Subarachnoid Hemorrhage Research
- Water Treatment and Disinfection
- Plant-Microbe Interactions and Immunity
- Biochemical and Structural Characterization
- Heme Oxygenase-1 and Carbon Monoxide
- Bacteriophages and microbial interactions
- Insect and Pesticide Research
- 3D Printing in Biomedical Research
- bioluminescence and chemiluminescence research
- Plant Stress Responses and Tolerance
Columbia University
2011-2018
ABSTRACT Many microbial species form multicellular structures comprising elaborate wrinkles and concentric rings, yet the rules governing their architecture are poorly understood. The opportunistic pathogen Pseudomonas aeruginosa produces phenazines, small molecules that act as alternate electron acceptors to oxygen nitrate oxidize intracellular redox state influence biofilm morphogenesis. Here, we show depth occupied by cells within colony biofilms correlates well with acceptor...
ABSTRACT Candida albicans has developmental programs that govern transitions between yeast and filamentous morphologies unattached biofilm lifestyles. Here, we report filamentation, intercellular adherence, development were inhibited during interactions Pseudomonas aeruginosa through the action of P. -produced phenazines. While phenazines are toxic to C. at millimolar concentrations, found lower concentrations any three different (pyocyanin, phenazine methosulfate, phenazine- 1 -carboxylate)...
In chronic infections, pathogens are often in the presence of other microbial species. For example, Pseudomonas aeruginosa is a common and detrimental lung pathogen individuals with cystic fibrosis (CF) co-infections Candida albicans common. Here, we show that P. biofilm formation phenazine production were strongly influenced by ethanol produced fungus C. albicans. Ethanol stimulated phenotypes indicative increased levels cyclic-di-GMP (c-di-GMP), c-di-GMP 2-fold higher ethanol. Through...
Diverse organisms secrete redox-active antibiotics, which can be used as extracellular electron shuttles by resistant microbes. Shuttle-mediated metabolism support survival when substrates are available not locally but rather at a distance. Such conditions arise in multicellular communities, where the formation of chemical gradients leads to resource limitation for cells depth. In pathogenic bacterium Pseudomonas aeruginosa PA14, antibiotics called phenazines act oxidants balance...
A major theme driving research in biology is the relationship between form and function. In particular, a longstanding goal has been to understand how evolution of multicellularity conferred fitness advantages. Here we show that biofilms bacterium Pseudomonas aeruginosa produce structures maximize cellular reproduction. Specifically, develop mathematical model resource availability metabolic response within colony features. This analysis accurately predicts measured distribution two types...
Chronic infections resulting from biofilm formation are difficult to eradicate with current antimicrobial agents and consequently new therapies needed. This work demonstrates that the carbon monoxide-releasing molecule CORM-2, previously shown kill planktonic bacteria, also attenuates surface-associated growth of Gram-negative pathogen Pseudomonas aeruginosa by both preventing maturation killing bacteria within established biofilm. CORM-2 treatment has an additive effect when combined...
Bacteria of many species rely on a simple molecule, the intracellular secondary messenger c-di-GMP (Bis-(3'-5')-cyclic dimeric guanosine monophosphate), to make vital choice: whether stay in one place and form biofilm, or leave it search better conditions. The network has bow-tie shaped architecture that integrates signals from outside world-the input stimuli-into levels then regulate genes for biofilm formation swarming motility-the output phenotypes. How does 'uninformed' process evolution...
Microbes in biofilms face the challenge of substrate limitation. In particular, oxygen often becomes limited for cells Pseudomonas aeruginosa growing laboratory or during host colonization. Previously we found that phenazines, antibiotics produced by P. aeruginosa, balance intracellular redox state biofilms. Here, show genes involved denitrification are induced phenazine-null (Δphz) mutant grown under an aerobic atmosphere, even absence nitrate. This finding suggests resident employ a...