A5103050309- CRISPR and Genetic Engineering
- Immune Response and Inflammation
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Virus-based gene therapy research
- Pancreatic function and diabetes
- Quantum Dots Synthesis And Properties
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Gold and Silver Nanoparticles Synthesis and Applications
- Liver physiology and pathology
- Electrochemical Analysis and Applications
- Immune cells in cancer
- Mesenchymal stem cell research
- Viral gastroenteritis research and epidemiology
- Immune Cell Function and Interaction
- Lanthanide and Transition Metal Complexes
- Electrohydrodynamics and Fluid Dynamics
- Nanopore and Nanochannel Transport Studies
- PARP inhibition in cancer therapy
- Molecular Junctions and Nanostructures
- Magnetism in coordination complexes
- Drug-Induced Hepatotoxicity and Protection
- Advanced Biosensing Techniques and Applications
- Bone Tissue Engineering Materials
Columbia University
2019-2023
Duke University
2008-2009
Duke-NUS Medical School
2008
Institute of Materials Research and Engineering
1998-2004
National University of Singapore
1997-2003
Indiana University Bloomington
1999
Mesenchymal stem cell (MSC) has been increasingly applied to cancer therapy because of its tumor-tropic capability. However, short retention at target tissue and limited payload option hinder the progress MSC-based therapy. Herein, we proposed a hybrid spheroid/nanomedicine system, comprising MSC spheroid entrapping drug-loaded nanocomposite, address these limitations. Spheroid formulation enhanced MSC's tumor tropism facilitated loading different types therapeutic payloads. This system...
Abstract Periodontitis is a common type of inflammatory bone loss and risk factor for systemic diseases. The pathogenesis periodontitis involves dysregulation, which represents target new therapeutic strategies to treat periodontitis. After establishing the correlation cell-free DNA (cfDNA) level with in patient samples, we test hypothesis that cfDNA-scavenging approach will benefit treatment. We create nanoparticulate cfDNA scavenger specific by coating selenium-doped hydroxyapatite...
Circulating cell-free DNA (cfDNA) released by damaged cells causes inflammation and has been associated with the progression of sepsis. One proposed strategy to treat sepsis is scavenge this inflammatory circulating cfDNA. Here, we develop a cfDNA-scavenging nanoparticle (NP) that consists cationic polyethylenimine (PEI) different molecular weight grafted zeolitic imidazolate framework-8 (PEI-g-ZIF) in simple one-pot process. PEI-g-ZIF NPs fabricated using PEI 1800 25k but not 600 suppressed...
Effective delivery of the CRISPR-Cas9 components is crucial to realizing therapeutic potential. Although many approaches have been developed for this application, oral has not explored due degradative nature gastrointestinal tract. For issue, we a series novel phenylboronic acid (PBA)-functionalized chitosan-polyethylenimine (CS-PEI) polymers CRISPR delivery. PBA functionalization equipped polyplex with higher stability, smooth transport across mucus, and efficient endosomal escape cytosolic...
Gene editing in the brain has been challenging because of restricted transport imposed by blood–brain barrier (BBB). Current approaches mainly rely on local injection to bypass BBB. However, such administration is highly invasive and not amenable treating certain delicate regions brain. We demonstrate a safe effective gene technique using focused ultrasound (FUS) transiently open BBB for intravenously delivered CRISPR/Cas9 machinery
Photothermal therapy (PTT) is an effective treatment modality that highly selective for tumor suppression and a hopeful alternative to traditional cancer therapy. However, PTT-induced inflammatory responses may result in undesirable side effects including increased risks of recurrence metastasis. Here we developed multifunctional MnO nanoparticles as scavengers proinflammatory molecules alleviate the response. The improve PTT by (1) binding scavenging inhibit molecule-induced Toll-like...
An effective photochemical route is reported here for size-controlled synthesis of nonaqueous colloidal gold particles in a highly polar and viscous medium, formamide the presence poly(vinylpyrrolidone). The photoreduction AuCl4- system attributed to higher degree dissociation direct reduction by photogenerated free radicals from coordinated molecules. Evident formation was not achieved dimethylformamide because cannot be dissociated dimethylformamide, having no amino group but an aldehyde...
For patients carrying
In this paper, we report a simple and clean method of producing uniform nonaqueous gold colloids by the chemical reduction chloroauric acid (HAuCl4) using oxygen-free organic supporting medium formamide itself as reducing agent in presence stabilizer poly(vinylpyrrolidone) (PVP). The HAuCl4 can take place easily at room temperature new system without introducing foreign agents. A two-step reaction process to particles was suggested from kinetics analysis for formation systems. difference...
Molecular-based magnet nickel−iron cyanide, K0.8Ni1.1[Fe(CN)6]·4.5H2O, was prepared by coprecipitation. The powder X-ray diffraction indicates an fcc crystal structure with a unit cell constant of 10.4 Å. Its Curie temperature determined to be 28 K from the Curie−Weiss law. effects heat treatment on microstructures as well magnetic properties in compound were studied. Several structures encountered during course annealing room 600 °C. conversion ferromagnetic FeIII-CN−NiII linkage...
Gene editing in the mammalian brain has been challenging because of restricted transport imposed by blood-brain barrier (BBB). Current approaches rely on local injection to bypass BBB. However, such administration is highly invasive and not amenable treating certain delicate regions brain. We demonstrate a safe effective gene technique using focused ultrasound (FUS) transiently open BBB for intravenously delivered CRISPR/Cas9 machinery
Abstract Gene editing in the mammalian brain has been challenging because of restricted transport imposed by blood-brain barrier (BBB). Current approaches rely on local injection to bypass BBB. However, such administration is highly invasive and not amenable treating certain delicate regions brain. We demonstrate a safe effective gene technique using focused ultrasound (FUS) transiently open BBB for intravenously delivered CRISPR/Cas9 machinery
Abstract Periodontitis is a common type of inflammatory bone loss and risk factor for systemic diseases. The pathogenesis periodontitis relies on dysregulation, which represents target new therapeutic strategies to treat periodontitis. Here we demonstrate that cell-free DNA (cfDNA) correlated with in patient samples, cfDNA/TLR9 interactions participate the immune response We then tested hypothesis removing cfDNA would benefit treatment. To create nucleic acid-binding nanoparticles (NABNs)...