A5002452282- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Advanced Fluorescence Microscopy Techniques
- Micro and Nano Robotics
- Image Processing Techniques and Applications
- Advanced Sensor and Energy Harvesting Materials
- Advanced Materials and Mechanics
- Viral Infectious Diseases and Gene Expression in Insects
- Neuroscience and Neuropharmacology Research
- Molecular Biology Techniques and Applications
- Multiple Sclerosis Research Studies
- Advanced Electron Microscopy Techniques and Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Anesthesia and Neurotoxicity Research
- Photoacoustic and Ultrasonic Imaging
- Nanoplatforms for cancer theranostics
- AI in cancer detection
- Autism Spectrum Disorder Research
- Modular Robots and Swarm Intelligence
- Force Microscopy Techniques and Applications
- Silicone and Siloxane Chemistry
- Corrosion Behavior and Inhibition
- Optical Imaging and Spectroscopy Techniques
New York Stem Cell Foundation
2020-2024
Karolinska Institutet
2017-2023
NYU Langone Health
2019-2021
Brigham and Women's Hospital
2017-2020
Harvard University
2017-2020
Neuroscience Institute
2020
Innovation Research Center
2018
Harvard–MIT Division of Health Sciences and Technology
2017-2018
Massachusetts Institute of Technology
2018
Columbia University
2018
Abstract To create life‐like movements, living muscle actuator technologies have borrowed inspiration from biomimetic concepts in developing bioinspired robots. Here, the development of a soft robotics system, with integrated self‐actuating cardiac muscles on hierarchically structured scaffold flexible gold microelectrodes is reported. Inspired by movement organisms, batoid‐fish‐shaped substrate designed and reported, which composed two micropatterned hydrogel layers. The first layer...
Advances in tissue clearing and molecular labeling methods are enabling unprecedented optical access to large intact biological systems. These developments fuel the need for high-speed microscopy approaches image samples quantitatively at high resolution. While light sheet (LSM), with its planar imaging speed low photo-bleaching, can be effective, scaling up larger volumes has been hindered by use of orthogonal illumination. To address this fundamental limitation, we have developed theta...
Tissue engineering has emerged as a viable approach to treat disease or repair damage in tissues and organs. One of the key elements for success tissue is use scaffold serving artificial extracellular matrix (ECM). The ECM hosts cells improves their survival, proliferation, differentiation, enabling formation new tissue. Here, we propose development class protein/polysaccharide-based porous scaffolds substitutes cardiac engineering. Scaffolds based on blends protein component, collagen...
Abstract We utilized forebrain organoids generated from induced pluripotent stem cells of patients with a syndromic form Autism Spectrum Disorder (ASD) homozygous protein-truncating mutation in CNTNAP2 , to study its effects on embryonic cortical development. Patients this present clinical characteristics brain overgrowth. Patient-derived displayed an increase volume and total cell number that is driven by increased neural progenitor proliferation. Single-cell RNA sequencing revealed...
Drug discovery for diseases such as Parkinson's disease are impeded by the lack of screenable cellular phenotypes. We present an unbiased phenotypic profiling platform that combines automated cell culture, high-content imaging, Cell Painting, and deep learning. applied this to primary fibroblasts from 91 patients matched healthy controls, creating largest publicly available Painting image dataset date at 48 terabytes. use fixed weights a convolutional neural network trained on ImageNet...
Multiple sclerosis (MS) is considered an inflammatory and neurodegenerative disease of the central nervous system, typically resulting in significant neurological disability that worsens over time. While considerable progress has been made defining immune system's role MS pathophysiology, contribution intrinsic CNS-cell dysfunction remains unclear. Here, we generated largest reported collection iPSC lines from people with spanning diverse clinical subtypes differentiated them into...
Bioinspired soft robotic systems that mimic living organisms using engineered muscle tissue and biomaterials are revolutionizing the current biorobotics paradigm, especially in biomedical research. Recreating artificial life-like actuation dynamics is crucial for a soft-robotic system. However, precise control tuning of behavior still represents one main challenges modern systems. This method describes low-cost, highly scalable, easy-to-use procedure to fabricate an electrically controllable...
Drug discovery for diseases such as Parkinson’s disease are impeded by the lack of screenable cellular phenotypes. We present an unbiased phenotypic profiling platform that combines automated cell culture, high-content imaging, Cell Painting, and deep learning. applied this to primary fibroblasts from 91 patients matched healthy controls, creating largest publicly available Painting image dataset date at 48 terabytes. use fixed weights a convolutional neural network trained on ImageNet...
Bioinspired soft robotic systems that mimic living organisms using engineered muscle tissue and biomaterials are revolutionizing the current biorobotics paradigm, especially in biomedical research. Recreating artificial life-like actuation dynamics is crucial for a soft-robotic system. However, precise control tuning of behavior still represents one main challenges modern systems. This method describes low-cost, highly scalable, easy-to-use procedure to fabricate an electrically controllable...
ABSTRACT Autism spectrum disorder (ASD) represents a major public health burden but translating promising treatment findings from preclinical non-human models of ASD to the clinic has remained challenging. The recent development forebrain organoids generated human induced pluripotent stem cells (hiPSCs) derived subjects with brain disorders is method study human-specific neurobiology, and may facilitate novel therapeutics. In this study, we utilized hiPSCs patients Old Order Amish community...
Abstract The increasing use of automation in cellular assays and cell culture presents significant opportunities to enhance the scale throughput imaging assays, but do so, reliable data quality consistency are critical. Realizing full potential will thus require design robust analysis pipelines that span entire workflow question. Here we present FocA, a deep learning tool that, near real-time, identifies in-focus out-of-focus images generated on fully automated biology research platform,...
There is an urgent need for simple and non-invasive identification of live neural stem/progenitor cells (NSPCs) in the developing adult brain as well disease, such tumors, due to potential clinical importance prognosis, diagnosis, treatment diseases nervous system. Here, we report a luminescent conjugated oligothiophene (LCO), named p-HTMI, non-amplified real-time detection human patient-derived glioblastoma (GBM) stem cell-like NSPCs. While p-HTMI stained only small fraction other cell...
A bioinspired soft robot is designed by mimicking the shape and biomechanical model of a batoid fish, as described Su Ryon Shin, Ali Khademhosseini, co-workers in article number 1704189. The biomimetic structure fabricated self-actuating cardiac muscles on hierarchically structured hydrogel scaffold with flexible gold microelectrodes. shows controllable actuating motion aligned direction contractile force cardiomyocytes localized electrical stimulation via
Abstract Advances in tissue clearing and molecular labelling methods are enabling unprecedented optical access to large intact biological systems. These advances fuel the need for high-speed microscopy approaches image samples quantitatively at high resolution. While Light Sheet Microscopy (LSM), with its planar imaging speed low photo-bleaching, can be effective, scaling up larger volumes has been hindered by use of orthogonal light-sheet illumination. To address this fundamental...
Abstract Leveraging artificial intelligence (AI) in image-based morphological profiling of cell populations is proving increasingly valuable for identifying diseased states and drug responses high-content imaging (HCI) screens. When the differences between (such as a healthy diseased) are completely unknown undistinguishable by human eye, it crucial that HCI screens large scale, allowing numerous replicates developing reliable models, well accounting confounding factors such individual...
The increasing use of automation in cellular assays and cell culture presents significant opportunities to enhance the scale throughput imaging assays, but do so, reliable data quality consistency are critical. Realizing full potential will thus require design robust analysis pipelines that span entire workflow question. Here we present FocA, a deep learning tool that, near real-time, identifies in-focus out-of-focus images generated on fully automated biology research platform, NYSCF Global...
Abstract Here we report a luminescent conjugated oligothiophene (LCO), named p-HTMI, for non-invasive and non-amplified real-time detection of live human patient-derived glioblastoma (GBM) cells embryonic neural stem (NSCs). While p-HTMI stained only small fraction other cell types investigated, the mere addition to culture resulted in efficient NSCs or GBM from rodents humans within minutes. is functionalized with methylated imidazole moiety resembling side chain histidine/histamine,...
Abstract Glioblastoma (GBM) is an aggressive nervous system tumor with a mean survival time of 12-14 months. Cells neural stem cell-like properties can be derived from GBM tumors. These cells seem to escape conventional irradiation treatment, chemotherapy, and surgery, may play crucial role for relapse. It therefore urgent develop novel approaches reliable detection in GBM. Here we report luminescent conjugated oligothiophene (LCO), named GlioStem (p-HTMI), non-invasive non-amplified...