A5016685068- Single-cell and spatial transcriptomics
- Cell Image Analysis Techniques
- Pluripotent Stem Cells Research
- Stress Responses and Cortisol
- Molecular Biology Techniques and Applications
- Hormonal Regulation and Hypertension
- Prenatal Screening and Diagnostics
- Cardiac Fibrosis and Remodeling
- Neuroinflammation and Neurodegeneration Mechanisms
- Urban, Neighborhood, and Segregation Studies
- Reproductive Biology and Fertility
- Adrenal Hormones and Disorders
- MicroRNA in disease regulation
- Congenital heart defects research
- Neurogenesis and neuroplasticity mechanisms
- AI in cancer detection
- Homelessness and Social Issues
- Renal and related cancers
- Prion Diseases and Protein Misfolding
- Genomic variations and chromosomal abnormalities
- CRISPR and Genetic Engineering
- Signaling Pathways in Disease
- Reading and Literacy Development
- Genetics and Neurodevelopmental Disorders
- Biomedical Ethics and Regulation
New York Stem Cell Foundation
2014-2024
Medical College of Wisconsin
2022
Drug discovery for diseases such as Parkinson's disease are impeded by the lack of screenable cellular phenotypes. We present an unbiased phenotypic profiling platform that combines automated cell culture, high-content imaging, Cell Painting, and deep learning. applied this to primary fibroblasts from 91 patients matched healthy controls, creating largest publicly available Painting image dataset date at 48 terabytes. use fixed weights a convolutional neural network trained on ImageNet...
Abstract Post-traumatic stress disorder (PTSD) can develop following severe trauma, but the extent to which genetic and environmental risk factors contribute individual clinical outcomes is unknown. Here, we compared transcriptional responses hydrocortisone exposure in human induced pluripotent stem cell (hiPSC)-derived glutamatergic neurons peripheral blood mononuclear cells (PBMCs) from combat veterans with PTSD ( n = 19 hiPSC 20 PBMC donors) controls donors). In only, observed...
STUDY QUESTIONWhat is the prevalence and developmental significance of morphologic nuclear abnormalities in human preimplantation embryos?
Astrocytes are instrumental in maintaining central nervous system (CNS) homeostasis and responding to injury. A major limitation of studying neurodegenerative diseases like multiple sclerosis (MS) is lack human pathological specimens obtained during the acute stages, thereby relegating research post-mortem years after initiation pathology. Rodent reactive astrocytes have been shown be cytotoxic neurons oligodendrocytes but may differ from cells, especially with genetic susceptibility....
Induced pluripotent stem cells (iPSCs) derived from patients with neurodegenerative disease generally lack neuropathological confirmation, the gold standard for classification and grading of severity. The use tissue a definitive diagnosis would be an ideal source iPSCs. challenge to this approach is that majority biobanked brain was not meant growing live cells, thus frozen in presence cryoprotectants such as DMSO. We report generation iPSCs non-cryoprotected dural stored at −80°C up 11...
Drug discovery for diseases such as Parkinson’s disease are impeded by the lack of screenable cellular phenotypes. We present an unbiased phenotypic profiling platform that combines automated cell culture, high-content imaging, Cell Painting, and deep learning. applied this to primary fibroblasts from 91 patients matched healthy controls, creating largest publicly available Painting image dataset date at 48 terabytes. use fixed weights a convolutional neural network trained on ImageNet...
Amino-terminal (Nt-) acetylation (NTA) is a common protein modification, affecting 80% of cytosolic proteins in humans. The human essential gene,