A5059107674- Fungal and yeast genetics research
- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- RNA and protein synthesis mechanisms
- SARS-CoV-2 detection and testing
- Advanced Fluorescence Microscopy Techniques
- Photosynthetic Processes and Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Biosensors and Analytical Detection
- Cellular transport and secretion
- Cell Image Analysis Techniques
- CRISPR and Genetic Engineering
- Peptidase Inhibition and Analysis
- SARS-CoV-2 and COVID-19 Research
- RNA modifications and cancer
- Plant-Microbe Interactions and Immunity
- Quantum and electron transport phenomena
- Mycorrhizal Fungi and Plant Interactions
- DNA Repair Mechanisms
- Advanced biosensing and bioanalysis techniques
- Semiconductor Quantum Structures and Devices
- Plant Reproductive Biology
- Yeasts and Rust Fungi Studies
Heidelberg University
2015-2024
DKFZ-ZMBH Alliance
2015-2024
Heidelberg University
2024
German Cancer Research Center
2014-2023
Heinrich Heine University Düsseldorf
2021
University Hospital Bonn
2021
German Center for Infection Research
2020
National University of Singapore
2019
Hong Kong University of Science and Technology
2019
University of Hong Kong
2019
Abstract Tagging of genes by chromosomal integration PCR amplified cassettes is a widely used and fast method to label proteins in vivo the yeast Saccharomyces cerevisiae . This strategy directs tags desired loci due flanking homologous sequences provided PCR‐primers, thus enabling selective introduction any sequence at place gene, e.g. for generation C‐terminal tagged or exchange promoter N‐terminal tagging gene. To make this most powerful we constructed series 76 novel cassettes,...
Epitope tagging of proteins as a strategy for the analysis function, interactions and subcellular distribution has become widely used. In yeast Saccharomyces cerevisiae, molecular biological techniques have been developed that use simple PCR-based to introduce epitope tags chromosomal loci (Wach et al., 1994). To further employ power this strategy, variety novel was constructed. These were combined with different selectable marker genes, resulting in PCR amplificable modules. Only one set...
A colorimetric isothermal RNA amplification method was shown to detect SARS-CoV-2 in clinical samples with excellent sensitivity and specificity.
The fate of a mutant form each the two yeast vacuolar enzymes proteinase yscA (PrA) and carboxypeptidase yscY (CPY) has been investigated. Both proteins are rapidly degraded after entering secretory pathway. Mutant PrA is deleted in 37 amino acids spanning processing site region pro‐peptide. enzyme shows no activity towards maturation itself or other hydrolases, function wild‐type PrA. CPY carries an Arg instead Gly residue highly conserved region, positions distant from active‐site Ser. In...
Integrative, centromeric, and episomal plasmids are essential for easy, fast, reliable genetic manipulation of yeast. We constructed a system shuttle vectors based on the widely used pRS series. genes conferring resistance to Geneticin® (kanMX4), nourseothricin (natNT2), hygromycin B (hphNT1) as markers. The centromeric that we can be same way traditional auxotrophic marker-based (pRS41x andpRS42x series). Additionally, created set nine yeast integrative with three dominant These allow...
Article5 December 2019Open Access ESCRT machinery mediates selective microautophagy of endoplasmic reticulum in yeast Jasmin A Schäfer DKFZ-ZMBH Alliance and CellNetworks Cluster Excellence, Center for Molecular Biology Heidelberg University (ZMBH), Heidelberg, Germany Search more papers by this author Julia P Schessner Peter W Bircham Takuma Tsuji Research Institute Diseases Old Age, Juntendo Graduate School Medicine, Tokyo, Japan Charlotta Funaya Electron Microscopy Core Facility,...
Rapid large-scale testing is essential for controlling the ongoing pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The standard diagnostic pipeline SARS-CoV-2 presence in patients with an infection predominantly based on pharyngeal swabs, from which viral RNA extracted using commercial kits, followed by reverse transcription and quantitative PCR detection. As a result large demand testing, extraction kits may be limited and, alternatively, non-commercial protocols...
The fate of genes after duplication Gene within an organism is a relatively common event during evolution. However, we cannot predict the duplicated genes: Will they be lost, evolve, or overlap in function organismal lineage species? Kuzmin et al. explored gene yeast Saccharomyces cerevisiae (see Perspective by Ehrenreich). They examined how experimental deletions one two (paralogs) affected fitness and were able to determine which have likely evolved new essential functions retained...
Tail-anchored (TA) proteins insert post-translationally into the endoplasmic reticulum (ER), outer mitochondrial membrane (OMM) and peroxisomes. Whereas GET pathway controls ER-targeting, no dedicated factors are known for OMM insertion, posing question of how accuracy is achieved. The AAA-ATPase Msp1 removes mislocalized TA from OMM, but it unclear, clients targeted degradation. Here we screened involved in degradation to mitochondria. We show that ER-associated (ERAD) E3 ubiquitin ligase...
Cell proliferation exerts a high demand on protein synthesis, yet the mechanisms coupling two processes are not fully understood. A kinase and phosphatase screen for activators of translation, based formation stress granules in human cells, revealed cell cycle–associated kinases as major candidates. CDK1 was identified positive regulator global synchronization experiments showed that this is an extramitotic function CDK1. Different pathways including eIF2α, 4EBP, S6K1 signaling contribute to...