A5002328566- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Cell Image Analysis Techniques
- Microtubule and mitosis dynamics
- Fungal and yeast genetics research
- AI in cancer detection
- Medical Image Segmentation Techniques
- Semiconductor materials and interfaces
- RNA regulation and disease
- Inorganic Chemistry and Materials
- Surface and Thin Film Phenomena
- Enzyme Structure and Function
- Molecular Junctions and Nanostructures
- Genomics and Chromatin Dynamics
- Nuclear Structure and Function
- Intermetallics and Advanced Alloy Properties
- Peptidase Inhibition and Analysis
- Gene Regulatory Network Analysis
- Metallurgical and Alloy Processes
- Single-cell and spatial transcriptomics
- Neural Networks and Applications
- Advanced Thermoelectric Materials and Devices
- Biochemical and Molecular Research
- Genetics, Aging, and Longevity in Model Organisms
ETH Zurich
2014-2024
Second Genome (United States)
2024
University of Basel
2024
École Polytechnique Fédérale de Lausanne
2023
Friedrich Miescher Laboratory
2009-2016
Max Planck Society
1976-2016
The cytoplasmic abundance of mRNAs is strictly controlled through a balance production and degradation. Whereas the control mRNA synthesis transcription has been well characterized, less known about regulation turnover, consensus model explaining wide variations in decay rates remains elusive. Here, we combine non-invasive transcriptome-wide stability measurements with selective acute perturbations to demonstrate that degradation tightly coupled translation, competition between translation...
Translational repression and mRNA degradation are critical mechanisms of posttranscriptional gene regulation that help cells respond to internal external cues. In response certain stress conditions, many decay factors enriched in processing bodies (PBs), cellular structures involved and/or storage mRNAs. Yet, how regulate assembly disassembly PBs remains poorly understood. Here, we show budding yeast, mutations the DEAD-box ATPase Dhh1 prevent ATP hydrolysis, or affect interaction between...
The binding of sequence-specific RNA-interacting proteins, such as the bacteriophage MS2 or PP7 coat to their corresponding target sequences has been extremely useful and widely used visualize single mRNAs in vivo. However, introduction stem-loops into yeast recently shown lead accumulation RNA fragments, suggesting that loops impair mRNA decay. This result was questioned, because fragment occurrence mainly assessed using ensemble methods, cellular localization its implications had not...
Abstract When cells encounter environmental stress, they rapidly mount an adaptive response by switching from pro-growth to stress-responsive gene expression programs. It is poorly understood how selectively silence pre-existing, transcripts, yet efficiently translate transcriptionally-induced stress mRNA, and whether these transcriptional post-transcriptional responses are coordinated. Here, we show that following acute glucose withdrawal in S. cerevisiae, pre-existing mRNAs not first...
Scientific Report29 January 2014Open Access Source Data Mad1 contribution to spindle assembly checkpoint signalling goes beyond presenting Mad2 at kinetochores Stephanie Heinrich Friedrich Miescher Laboratory of the Max Planck Society, Tübingen, Germany Search for more papers by this author Katharina Sewart Hanna Windecker Maria Langegger Nadine Schmidt Nicole Hustedt Silke Hauf Corresponding Author Information Heinrich1, Sewart1,2, Windecker1,3, Langegger1, Schmidt1, Hustedt1,4 and 1,2...
The spindle assembly checkpoint (SAC) blocks entry into anaphase until all chromosomes have stably attached to the mitotic through their kinetochores. signal originates from unattached kinetochores, where SAC proteins enrich. Whether enrichment of is crucial for signalling unclear. Here we provide evidence that in fission yeast, recruitment kinase Mph1 vital importance a stable arrest. An mutant eliminates kinetochore abolishes signalling, whereas forced this kinetochores restores...
Summary Fluorescent proteins and peptide tags are essential tools in cellular biology, but can alter the biochemical properties of target proteins. Biomolecular condensates, which have emerged as key principles organization, suggested to provide robustness cells, yet they also respond sensitively small changes environmental conditions—or tagging their components, our findings suggest. Here, we investigated effects sixteen widely used on condensate formation various model organisms, vitro ,...
The eukaryotic spindle assembly checkpoint (SAC) delays anaphase in the presence of chromosome attachment errors. Bub3 has been reported to be required for SAC activity all eukaryotes examined so far. We find that Bub3, unlike its binding partner Bub1, is not essential fission yeast. As needed efficient kinetochore localization and Mad1, Mad2 Mad3, this implies most proteins do need enriched at kinetochores function. also dispensable shugoshin centromeres, which second known function Bub1....
Nuclear mRNA export via nuclear pore complexes is an essential step in eukaryotic gene expression. Although factors involved transport have been characterized, a comprehensive mechanistic understanding of this process and its regulation lacking. Here, we use single-RNA imaging yeast to show that cells retention control during stress. We demonstrate that, upon glucose withdrawal, the RNA-binding factor Nab2 forms RNA-dependent condensate-like structures nucleus. This coincides with reduced...
PLA2G6-Associated Neurodegeneration (PLAN) comprises three diseases with overlapping features: infantile neuroaxonal dystrophy (INAD, OMIM 256600, ORPHA: 35069), atypical (atypical NAD, 610217), and PLA2G6-related dystonia-parkinsonism (PARK14, 612953), which are caused by recessive variants in the PLA2G6 gene. INAD is an early onset disorder associated severe symptoms including ataxia, mental motor deterioration, hypotonia, progressive spastic tetraparesis, visual impairments, bulbar...
Abstract The organization and biophysical properties of the cytoplasm influence all cellular reactions, including molecular interactions mobility biomolecules. It has become clear that does not behave like a simple fluid but instead is densely crowded highly organized environment. Yet, detailed cytoplasm, mechanisms control them how they biochemistry cells remain poorly understood. Here, we investigate diffusive in silico vivo, employing mRNPs ( m essenger r ibo n ucleo p rotein) GEM g...
Abstract The cytoplasmic abundance of mRNAs is strictly controlled through a balance production and degradation. Whereas the control mRNA synthesis transcription has been well characterized, less known about regulation turnover, consensus model explaining wide variations in decay rates remains elusive. Here, we combine non-invasive transcriptome-wide stability measurements with selective acute perturbations to demonstrate that degradation tightly coupled translation, competition between...
Abstract Motivation: The statistical analysis of single-cell data is a challenge in cell biological studies. Tailored models and computational methods are required to resolve the subpopulation structure, i.e. correctly identify characterize subpopulations. These approaches also support unraveling sources cell-to-cell variability. Finite mixture have shown promise, but available ill suited simultaneous consideration from multiple experimental conditions censored data. prevalence relevance...