A5041272701- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Ubiquitin and proteasome pathways
- Chromosomal and Genetic Variations
- DNA Repair Mechanisms
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Fungal and yeast genetics research
- Genetics, Bioinformatics, and Biomedical Research
- Cellular transport and secretion
- Genomics and Phylogenetic Studies
- Plant Genetic and Mutation Studies
- Plant Disease Resistance and Genetics
- Advanced Proteomics Techniques and Applications
- Plant Molecular Biology Research
- Gene Regulatory Network Analysis
- Cell Image Analysis Techniques
- Epigenetics and DNA Methylation
- Cellular Mechanics and Interactions
- Reproductive Biology and Fertility
- Trypanosoma species research and implications
- Bioinformatics and Genomic Networks
- Mass Spectrometry Techniques and Applications
- Cancer-related Molecular Pathways
- Signaling Pathways in Disease
Virginia Tech
2014-2024
Landesgesundheitsamt Baden-Württemberg
2023
Bayer (Germany)
2023
Biocom
2016-2019
Max Planck Society
2008-2017
Friedrich Miescher Laboratory
2007-2017
Research Institute of Molecular Pathology
2000-2006
The University of Tokyo
2004-2005
The proper segregation of sister chromatids in mitosis depends on bipolar attachment all chromosomes to the mitotic spindle. We have identified small molecule Hesperadin as an inhibitor chromosome alignment and segregation. Our data imply that causes this phenotype by inhibiting function kinase Aurora B. Mammalian cells treated with enter anaphase presence numerous monooriented chromosomes, many which may both kinetochores attached one spindle pole (syntelic attachment). also arrested taxol...
Cell division depends on the separation of sister chromatids in anaphase. In yeast, is initiated by cleavage cohesin protease separase. vertebrates, most removed from chromosome arms a cleavage-independent mechanism. Only residual amounts are cleaved at onset anaphase, coinciding with its disappearance centromeres. We have identified two separase sites human subunit SCC1 and conditionally expressed noncleavable mutants cells. Our results indicate that essential for chromatid completion cytokinesis.
Cohesin is a protein complex that required to hold sister chromatids together. Cleavage of the Scc1 subunit cohesin by protease separase releases from chromosomes and thereby enables separation in anaphase. In vertebrate cells, bulk dissociates chromosome arms already during prophase prometaphase without cleavage Scc1. Polo-like kinase 1 (Plk1) Aurora-B are for this dissociation process, Plk1 can phosphorylate subunits SA2 vitro, consistent with possibility phosphorylation triggers arms....
Fission yeast shugoshin Sgo1 is meiosis specific and cooperates with protein phosphatase 2A to protect centromeric cohesin at I. The other shugoshin-like Sgo2, which requires the heterochromatin Swi6/HP1 for full viability, plays a crucial role proper chromosome segregation both mitosis meiosis; however, underlying mechanisms are totally elusive. We here demonstrate that, unlike Sgo1, Sgo2 dispensable protection of cohesin. Instead, interacts Bir1/Survivin promotes Aurora kinase complex...
To quantify cell cycle-dependent fluctuations on a proteome-wide scale, we performed integrative analysis of the proteome and phosphoproteome during four major phases cycle in Schizosaccharomyces pombe. In highly synchronized cells, identified 3753 proteins 3682 phosphorylation events relatively quantified 65% data across all phases. Quantitative changes were infrequent weak but prominent phosphoproteome. Protein peaked mitosis, where median site occupancy was 44%, about 2-fold higher than...
System-wide analysis of proteins phosphorylated by the mitotic kinase Aurora in fission yeast suggests widespread functions for this regulating chromatin dynamics.
Gene expression inherently gives rise to stochastic variation ("noise") in the production of gene products. Minimizing noise is crucial for ensuring reliable cellular functions. However, cannot be suppressed below a certain intrinsic limit. For constitutively expressed genes, this limit typically assumed Poissonian noise, wherein variance mRNA numbers equal their mean. Here, we demonstrate that several cell division genes fission yeast exhibit variances significantly The reduced can...
Scientific Report29 January 2014Open Access Source Data Mad1 contribution to spindle assembly checkpoint signalling goes beyond presenting Mad2 at kinetochores Stephanie Heinrich Friedrich Miescher Laboratory of the Max Planck Society, Tübingen, Germany Search for more papers by this author Katharina Sewart Hanna Windecker Maria Langegger Nadine Schmidt Nicole Hustedt Silke Hauf Corresponding Author Information Heinrich1, Sewart1,2, Windecker1,3, Langegger1, Schmidt1, Hustedt1,4 and 1,2...
The spindle assembly checkpoint (SAC) blocks entry into anaphase until all chromosomes have stably attached to the mitotic through their kinetochores. signal originates from unattached kinetochores, where SAC proteins enrich. Whether enrichment of is crucial for signalling unclear. Here we provide evidence that in fission yeast, recruitment kinase Mph1 vital importance a stable arrest. An mutant eliminates kinetochore abolishes signalling, whereas forced this kinetochores restores...
The eukaryotic spindle assembly checkpoint (SAC) delays anaphase in the presence of chromosome attachment errors. Bub3 has been reported to be required for SAC activity all eukaryotes examined so far. We find that Bub3, unlike its binding partner Bub1, is not essential fission yeast. As needed efficient kinetochore localization and Mad1, Mad2 Mad3, this implies most proteins do need enriched at kinetochores function. also dispensable shugoshin centromeres, which second known function Bub1....
The perturbation of protein kinases with small organic molecules is a powerful approach to dissect kinase function in complex biological systems. Covalent inhibitors that target thiols the ATP binding pocket domain proved be ideal reagents for investigation highly dynamic cellular processes. However, due covalent inhibitors' possible off-target reactivities, it required overall shape inhibitor as well intrinsic reactivity electrophile are precisely tuned favor reaction only desired cysteine....