A5040690112- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Developmental Biology and Gene Regulation
- RNA modifications and cancer
- Cancer-related gene regulation
- Ubiquitin and proteasome pathways
- Cellular Mechanics and Interactions
- Animal Genetics and Reproduction
- Cell Adhesion Molecules Research
- Chromosomal and Genetic Variations
- Congenital heart defects research
- Protein Degradation and Inhibitors
- Cancer, Hypoxia, and Metabolism
- Viral Infectious Diseases and Gene Expression in Insects
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Mesenchymal stem cell research
- Hippo pathway signaling and YAP/TAZ
- Invertebrate Immune Response Mechanisms
- RNA regulation and disease
- Neurobiology and Insect Physiology Research
- melanin and skin pigmentation
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Neonatal Respiratory Health Research
Centro Andaluz de Biología del Desarrollo
2014-2025
Universidad Pablo de Olavide
2009-2024
Junta de Andalucía
2015-2024
Consejo Superior de Investigaciones Científicas
2007-2017
European Molecular Biology Organization
2013
University of Chicago
2012
Unidades Centrales Científico-Técnicas
2011
Centro Nacional de Biotecnología
2011
The generation of distinctive cell types that form different tissues and organs requires precise, temporal spatial control gene expression. This depends on specific cis -regulatory elements distributed in the noncoding DNA surrounding their target genes. Studies performed mammalian embryonic stem cells Drosophila embryos suggest active enhancers part a defined chromatin landscape marked by histone H3 lysine 4 mono-methylation (H3K4me1) 27 acetylation (H3K27ac). Nevertheless, little is known...
Abstract The identification and characterization of the regulatory activity genomic sequences is crucial for understanding how information contained in genomes translated into cellular function. cis ‐regulatory control when, where, much genes are transcribed can activate (enhancers) or repress (silencers) gene expression. Here, we describe a novel Tol2 transposon‐based vector assessing enhancer zebrafish ( Danio rerio ). This Zebrafish Enhancer Detector (ZED) harbors several key...
The order of genes in eukaryotic genomes has generally been assumed to be neutral, since gene is largely scrambled over evolutionary time. Only a handful exceptional examples are known, typically involving deeply conserved clusters tandemly duplicated (e.g., Hox and histones). Here we report the first systematic survey microsynteny conservation across metazoans, utilizing 17 genome sequences. We identified nearly 600 pairs unrelated that have remained tightly physically linked diverse...
Insulators are regulatory elements that help to organize eukaryotic chromatin via enhancer-blocking and barrier activity. Although there several examples of transposable element (TE)-derived insulators, the contribution TEs human insulators has not been systematically explored. Mammalian-wide interspersed repeats (MIRs) a conserved family have substantial capacity share sequence characteristics with tRNA-related insulators. We sought evaluate whether MIRs can serve as in genome. applied...
The existence of specialised regulatory microenvironments or niches that sustain stable stem cell populations is well documented in many tissues. However, the specific mechanisms by which niche support (or stromal) cells govern maintenance remain largely unknown. Here we demonstrate removal Jak/Stat pathway Drosophilaovarian leads to germline loss differentiation. Conversely,ectopic activation induces tumours,implying presence a signal relay between stromal compartment and population. We...
Recent genome-wide association studies have uncovered genomic loci that underlie an increased risk for atrial fibrillation, the major cardiac arrhythmia in humans. The most significant locus is located a gene desert at 4q25, approximately 170 kilobases upstream of PITX2, which codes transcription factor involved embryonic left-right asymmetry and development. However, how this region functionally structurally relates to PITX2 fibrillation unknown. To characterise its function, we tested...
Aging is one of the main challenges facing modern society. Understanding cellular processes that occur in later stages life essential to address age-related diseases. Although aging a global process affects entire body, brain most sensitive organs and affected by numerous degenerative In this study, we investigate cell types genes are particularly process. To end, performed time-course single-cell RNAseq experiment series killifish, vertebrate model with shortest lifespan, which makes it an...
The complex relationship between ontogeny and phylogeny has been the subject of attention controversy since von Baer’s formulations in 19th century. classic concept that embryogenesis progresses from clade general features to species-specific characters often revisited. It become accepted embryos a show maximum morphological similarity at so-called phylotypic period (i.e., during mid-embryogenesis). According hourglass model, body plan conservation would depend on constrained molecular...
Abstract Genetic defects such as copy number variations (CNVs) in non-coding regions containing conserved elements (CNEs) outside the transcription unit of their target gene, can underlie genetic disease. An example this is short stature homeobox ( SHOX ) regulated by seven CNEs located downstream and upstream , with proven enhancer capacity chicken limbs. CNVs have been reported many idiopathic (ISS) cases, however, only recently a few enhancers identified. Here, we set out to provide...
Meis1, a conserved transcription factor of the TALE-homeodomain class, is expressed in wide variety tissues during development. Its complex expression pattern likely to be controlled by an equally regulatory landscape. Here we have scanned Meis1 locus for elements and found 13 non-coding regions, highly between humans teleost fishes, that enhancer activity stable transgenic zebrafish lines. All these regions are syntenic most vertebrates. The composite all recapitulate early embryogenesis,...
Hypoxia inducible factor (HIF) up-regulates the transcription of a few hundred genes required for adaptation to hypoxia. This restricted set targets is in sharp contrast with widespread distribution HIF binding motif throughout genome. Here, we investigated transcriptional response GYS1 and RUVBL2 hypoxia understand mechanisms that restrict activity toward specific genes. are encoded by opposite DNA strands separated short intergenic region (~1 kb) contains functional element equidistant...
The generation of epithelial cell polarity is a key process during development. Although the induction and orientation by cell-cell cell-extracellular matrix (ECM) interactions well established, molecular mechanisms which signals from ECM control in developing tissues remain poorly understood. Here, we have used follicular epithelium <i>Drosophila</i> ovary to investigate role that integrins, main cell-ECM receptors, play establishment apicobasal polarity. Mature follicle cells an apical...
The self-organized morphogenesis of the vertebrate optic cup entails coupling activation retinal gene regulatory network to constriction-driven infolding epithelium. Yet genetic mechanisms underlying this coordination remain largely unexplored. Through phylogenetic footprinting and transgenesis in zebrafish, here we examine cis-regulatory landscape opo, an endocytosis regulator essential for eye morphogenesis. Among different conserved enhancers identified, isolate a single retina-specific...
Developmental and physiological processes depend on the transcriptional translational activity of heterogeneous cell populations. A main challenge in gene expression studies is dealing with this intrinsic complexity while keeping sequencing efficiency. Translating ribosome affinity purification (TRAP) methods have allowed cell-specific recovery polyribosome-associated RNAs by genetic tagging ribosomes selected Here we combined TRAP approach adapted enhancer trap (trap-TRAP) to systematically...
Abstract Control of gene expression is dictated by cell-type specific regulatory sequences that physically organize the structure chromatin, including promoters, enhancers and insulators. While promoters convey activating signals, insulators prevent cross-talk elements within adjacent loci safeguard specificity action towards their targets in a tissue manner. Using mouse tyrosinase ( Tyr) locus as an experimental model, whose mutations are associated with albinism, we described chromatin...
Abstract The cellular complexity of the endochondral bone underlies its essential and pleiotropic roles during organismal life. While adult has received significant attention, we still lack a deep understanding perinatal cellulome. Here, have profiled full composition murine at single‐cell level transition from fetal to newborn life in comparison with tissue, particular emphasis on mesenchymal compartment. contains different fibroblastic clusters blastema‐like characteristics organizing...