A5074442125- Bacterial Genetics and Biotechnology
- DNA Repair Mechanisms
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Fungal and yeast genetics research
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Escherichia coli research studies
- DNA and Nucleic Acid Chemistry
- Polar Research and Ecology
- Genomics and Chromatin Dynamics
- Protist diversity and phylogeny
- CRISPR and Genetic Engineering
- Antibiotic Resistance in Bacteria
- Enzyme Structure and Function
- Yeasts and Rust Fungi Studies
- Fermentation and Sensory Analysis
- Protein Structure and Dynamics
- Nuclear Structure and Function
- RNA modifications and cancer
- Biofuel production and bioconversion
- Microtubule and mitosis dynamics
- Neurological diseases and metabolism
- Photosynthetic Processes and Mechanisms
- Porphyrin Metabolism and Disorders
National Institute of Genetics
2016-2025
The Graduate University for Advanced Studies, SOKENDAI
2012-2023
NTL Institute for Applied Behavioral Science
2014
Research Organization of Information and Systems
2011
Japan Radioisotope Association
2006-2009
National Institute for Environmental Studies
2009
University of Miyazaki
2009
National Institute for Basic Biology
2009
Kumamoto University
1992-2001
University of Edinburgh
1992
The fission yeast clade--comprising Schizosaccharomyces pombe, S. octosporus, cryophilus, and japonicus--occupies the basal branch of Ascomycete fungi is an important model eukaryote biology. A comparative annotation these genomes identified a near extinction transposons associated innovation transposon-free centromeres. Expression analysis established that meiotic genes are subject to antisense transcription during vegetative growth, which suggests mechanism for their tight regulation. In...
To study the chromosomal partitioning mechanism in cell division, we have isolated a novel type of Escherichia coli mutants which formed anucleate cells, by using newly developed techniques. One them, named mukA1, is not lethal and produces normal-sized cells at frequency 0.5 to 3% total exponentially growing populations but does produce filamentous cells. Results suggest that mutant defective chromosome positioning regular intracellular positions fails frequently partition replicated...
Summary Fission of bacterial cells involves the co‐ordinated invagination envelope layers. Invagination cytoplasmic membrane (IM) and peptidoglycan (PG) layer is likely driven by septal ring organelle. outer (OM) in Gram‐negative species thought to occur passively via its tethering underlying PG with generally distributed PG‐binding OM (lipo)proteins. The Tol–Pal system energized proton motive force well conserved bacteria. It consists five proteins that can connect both IM layers protein–PG...
We have revealed the subcellular localization of different DNA segments that are located at ∼230-kb intervals on Escherichia coli chromosome using fluorescence in situ hybridization (FISH). The series is localized within cell same order as map. large region including oriC shows similar patterns, which we call Ori domain. In addition, pattern segment dif characteristic replication terminus region. also Ter newborn cells, and domains differentially near opposite poles. Subsequently, B period,...
The ftsH gene is essential for cell viability in Escherichia coli. We cloned and sequenced the wild-type temperature-sensitive ftsH1(Ts) gene. It was suggested that FtsH protein an integral membrane of 70.7 kDa (644 amino acid residues) with a putative ATP-binding domain. found to have two base substitutions within coding sequence corresponding Glu-463 by Lys Pro-587 Ala. Homology search revealed approximately 200-amino-acid domain, including sequence, highly homologous (35 48% identical)...
Hironori Niki and Sota Hiraga Department of Molecular Cell Biology, Institute Embryology Genetics, Kumamoto University School Medicine, 862-0976, Japan
The determination and regulation of cell morphology are critical components cell-cycle control, fitness, development in both single-cell multicellular organisms. Understanding how environmental factors, chemical perturbations, genetic differences affect requires precise, unbiased, validated measurements cell-shape features.Here we introduce two software packages, Morphometrics BlurLab, that together enable automated, computationally efficient, unbiased identification cells morphological...
ABSTRACT Assembly of the cell division apparatus in bacteria starts with formation Z ring on cytoplasmic face membrane. This process involves accumulation FtsZ polymers at midcell and their interaction several FtsZ-binding proteins that collectively organize into a membrane-associated ring-like configuration. Three such proteins, FtsA, ZipA, ZapA, have previously been identified Escherichia coli . FtsA ZipA are essential help connect inner ZapA is protein not required for fission per se but...
FtsH protein in Escherichia coli is an essential of 70.7 kDa (644 amino acid residues) with a putative ATP-binding sequence. Western blots (immunoblots) proteins from fractionated cell extracts and immunoelectron microscopy the FtsH-overproducing strain showed exclusive localization cytoplasmic membrane. Most FtsH-specific labeling gold particles was observed membrane adjacent cytoplasm; much less outer bulk cytoplasm. Genetic analysis by TnphoA insertions into ftsH revealed that 25- to...
Bacterial genes required for proper partitioning consist of two transacting that encode proteins and a cis-acting gene functions like centromere. Plasmids actively by means these migrate from midcell to the cell quarters are tethered sites until cells divide. Previously were mainly found on plasmids phages in Escherichia coli. However, progress genome sequencing reveals ubiquitous many bacterial chromosomes. Each homologue belongs family, ParA or ParB. Moreover, phylogenic analysis members...
We have isolated suppressor mutants that suppress temperature-sensitive colony formation and anucleate cell production of a mukB mutation. A linkage group (smbB) the mutations is located in rne/ams/hmp gene encoding processing endoribonuclease RNase E. All rne code for truncated E polypeptides lacking carboxyl-terminal half. The amount MukB protein was higher these than rne+ strain. These grew nearly normally mukB+ genetic background. copy number plasmid pBR322 lower isogenic results suggest...
We previously found that SeqA protein, which binds preferentially to newly replicated hemimethylated DNA, is localized as discrete fluorescent foci in Escherichia coli cells. A single focus, at midcell, separates into two and these migrate abruptly opposite directions.The present study shows (i) appearance of depends on continuous DNA replication, suggesting the represent clusters consisting (ii) a synchronous round focus midcell directions midway through replication from oriC terminus,...
Mini-F plasmid has the trans-acting genes sopA and sopB cis-acting site sopC which are essential for accurate partitioning of DNA molecules into both daughter cells. In this study, we purified independently SopA SopB proteins, analyzed in vitro DNA-binding activity these proteins by gel retardation assay, determined precise binding sites footprinting method. binds to four repeated sequences (CTTTGC) located promoter-operator region sopAB operon. The is enhanced addition protein. protein...
Summary The F plasmid is actively partitioned to daughter cells by the sopABC gene. To elucidate partitioning mechanisms, we simultaneously analysed movements of and SopA ATPase in single living cells. SopA, which a putative motor protein assembled densely near nucleoid borders formed discrete focus associated with less dense filamentous distribution along long axis cell. oscillates between cell poles. direction motion switches as its position. velocity stays constant while it moving towards...
Abstract Background DNA replication initiates at distinct origins in eukaryotic genomes, but the genomic features that define these sites are not well understood. Results We have taken a combined experimental and bioinformatic approach to identify characterize of three distantly related fission yeasts: Schizosaccharomyces pombe , octosporus japonicus . Using single-molecule deep sequencing construct amplification-free high-resolution profiles, we located identified sequence motifs predict...
Escherichia coli has an ability to assemble DNA fragments with homologous overlapping sequences of 15 40 bp at each end. Several modified protocols have already been reported improve this simple and useful cloning technology. However, the molecular mechanism by which E. accomplishes such is still unknown. In study, we provide evidence that in vivo independent both RecA RecET recombinases but dependent on XthA, a 3' 5' exonuclease. Here, XthA referred as vivoE. (iVEC). We also show iVEC...