A5003443257- Antibiotic Resistance in Bacteria
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Mycobacterium research and diagnosis
- Bacterial Identification and Susceptibility Testing
- Tuberculosis Research and Epidemiology
- Immune Response and Inflammation
- Hemoglobinopathies and Related Disorders
- Bacterial biofilms and quorum sensing
- Advanced Biosensing Techniques and Applications
- Clostridium difficile and Clostridium perfringens research
- Vibrio bacteria research studies
- Antimicrobial Resistance in Staphylococcus
- Bacillus and Francisella bacterial research
- Salmonella and Campylobacter epidemiology
- Iron Metabolism and Disorders
- COVID-19 Clinical Research Studies
- Bacterial Genetics and Biotechnology
- Viral gastroenteritis research and epidemiology
- Immunodeficiency and Autoimmune Disorders
- Blood disorders and treatments
- Erythrocyte Function and Pathophysiology
- Child Nutrition and Water Access
- Cytomegalovirus and herpesvirus research
- T-cell and B-cell Immunology
Brigham and Women's Hospital
2015-2025
Harvard University
2017-2024
Oregon Medical Research Center
2024
New York Proton Center
2024
University of Pittsburgh
2024
Communities In Schools of Orange County
2024
Lindsay Unified School District
2024
University of Rochester Medical Center
2018-2022
University of Rochester
2019-2022
University School
2002-2011
Summary Fission of bacterial cells involves the co‐ordinated invagination envelope layers. Invagination cytoplasmic membrane (IM) and peptidoglycan (PG) layer is likely driven by septal ring organelle. outer (OM) in Gram‐negative species thought to occur passively via its tethering underlying PG with generally distributed PG‐binding OM (lipo)proteins. The Tol–Pal system energized proton motive force well conserved bacteria. It consists five proteins that can connect both IM layers protein–PG...
Abstract TLR2 recognizes components of Mycobacterium tuberculosis (Mtb) and initiates responses by APCs that influence both innate adaptive immunity. Mtb lipoproteins are an important class ligand, but only two, LpqH LprG, have been characterized to date. In this study, we characterize a third lipoprotein, LprA, determine its effects on host macrophages dendritic cells. LprA is cell wall-associated lipoprotein with no homologs outside the slow-growing mycobacteria. Using smegmatis as...
ABSTRACT Carbapenem-resistant Enterobacteriaceae (CRE) are an urgent public health concern. Rapid identification of the resistance genes, their mobilization capacity, and strains carrying them is essential to direct hospital resources prevent spread improve patient outcomes. Whole-genome sequencing allows refined tracking both chromosomal traits associated mobile genetic elements that harbor genes. To enhance surveillance CREs, clinical isolates with phenotypic carbapenem antibiotics...
Abstract The past 10 years have brought paradigm-shifting changes to clinical microbiology. This paper explores the top transformative innovations across diagnostic spectrum, including not only state of art technologies but also preanalytic and post-analytic advances. Clinical decision support tools reshaped testing practices, curbing unnecessary tests. Innovations like broad-range polymerase chain reaction metagenomic sequencing, whole genome multiplex molecular panels, rapid phenotypic...
The success of Mycobacterium tuberculosis as a pathogen relies on its ability to regulate the host immune response. M. can manipulate adaptive T cell responses indirectly by modulating antigen-presenting (APC) function or directly interacting with cells. Little is known about role molecules in direct regulation function. Using biochemical approach, we identified lipoproteins LprG and LpqH major lysate responsible for costimulation primary human CD4(+) In absence APCs, activation memory cells...
Klebsiella aerogenes is a nosocomial pathogen associated with drug resistance and outbreaks in intensive care units. In 5-month period 2017, we experienced an increased incidence of cultures for carbapenem-resistant K. (CR-KA) from adult cardiothoracic unit (CICU) involving 15 patients.
To report a case of isolated vitritis as the presenting feature disseminated infection with Mycobacterium chelonae subspecies gwanakae, novel subspecies. Case report. A 66-year-old male who presented progressive floaters and blurred vision was found to have left eye. Diagnostic vitrectomy revealed acid-fast bacilli in vitreous. Whole genome sequencing identified organism recently The patient admitted for further evaluation treatment. Punch biopsies numerous ulcerative skin lesions bacilli....
The clinical laboratory continues to play a critical role in managing the coronavirus pandemic. Numerous US Food and Drug Administration emergency use authorization (EUA) laboratory-developed test (LDT) serologic assays have become available. performance characteristics of these their utility continue be defined real time during this AACC convened panel experts from chemistry, microbiology, immunology laboratories; vitro diagnostics industry; regulatory agencies provide practical...
The coronavirus disease 2019 (COVID-19) pandemic has highlighted the challenges inherent to serological detection of a novel pathogen such as severe acute respiratory syndrome 2 (SARS-CoV-2). Serological tests can be used diagnostically and for surveillance, but their usefulness depends on throughput, sensitivity, specificity. Here, we describe multiplex fluorescent microsphere-based assay, 3Flex, that detect antibodies three major SARS-CoV-2 antigens-spike (S) protein, spike ACE2...
A protective antibody response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is crucial decrease morbidity and mortality from disease 2019 (COVID-19) disease. The effects of preexisting anti-human (HCoV) antibodies on the SARS-CoV-2-specific immunoglobulin G (IgG) responses severity are currently unclear.We profiled anti-spike (S), S1, S2, receptor-binding domain IgG against SARS-CoV-2 6 HCoVs using a multiplex assay (mPLEX-CoV) with serum samples infected (n = 155)...
Many providers use severe acute respiratory coronavirus virus 2 (SARS-CoV-2) cycle thresholds (Ct values) as approximate measures of viral burden in association with other clinical data to inform decisions about treatment and isolation. We characterized temporal changes Ct values for non-SARS-CoV-2 viruses a first step determine whether could play similar role the management viruses.
Dim expression of CD5 on human B lymphocytes has been used to delineate B1 and B2 subsets. Nevertheless, others have suggested that the molecule is an activation marker does not predicate a subset distinction. We enzymatic amplification staining, technology enhances resolution flow cytometric analysis cell surface molecules by as much 100-fold, determine essentially all cells express CD5. Furthermore, we show this regulated during Epstein–Barr virus transformation.
Extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae pose significant treatment and infection prevention challenges. E. coli sequence type (ST)131 associated with the blaCTX-M-15 gene has been dominant lineage of ESBL-producing in US worldwide. In this study, our objective was to determine profile, means dissemination, prevalence, clonal identity region Western New York. Whole-genome SNP-based phylogenomics used assess 89 ceftriaxone-resistant (CTR) coli. Isolates were collected...
Staphylococcus aureus is a major cause of ocular infections, often resulting in devastating vision loss. Despite the significant morbidity associated with these little yet known regarding specific strain types that may have predilection for tissues nor set virulence factors drive its pathogenicity this biological niche. Whole genome sequencing (WGS) can provide valuable insight regard by providing prospective, comprehensive assessment and driving disease among subsets clinical isolates. As...
ABSTRACT Mycobacterium tuberculosis is a leading killer of HIV-infected individuals worldwide, particularly in sub-Saharan Africa, where it responsible for up to 50% HIV-related deaths. Infection by HIV predisposes M. infection, and coinfection accelerates the progression both diseases. In contrast most other opportunistic infections associated with HIV, an increased risk infection occurs during early-stage disease, long before CD4 T cell counts fall below critical levels. We hypothesized...
New Delhi metallo-beta-lactamases (NDMs) are an uncommon but emerging cause of carbapenem resistance in the United States. Genomic factors promoting their domestic spread remain poorly characterized. A prospective genomic surveillance program among Boston-area hospitals identified multiple new occurrences NDM-carrying strains Escherichia coli and Enterobacter cloacae complex inpatient outpatient settings, representing first NDM-mediated since initiating 2011. Cases included patients with no...
Infections caused by extended-spectrum β-lactamase (ESBL)-producing Escherichia coli are a significant cause of morbidity and health care costs. Globally, the prevailing clonal type is ST131 in association with bla CTX-M-15 gene.
Bacterial keratitis causes significant blindness, yet antimicrobial resistance has rendered current treatments ineffective. Polymyxin B-trimethoprim (PT) plus rifampin potent in vitro activity against Staphylococcus aureus and Pseudomonas aeruginosa, two important of keratitis. Here we further characterize this combination P. aeruginosa a murine model. PT performed comparably to or better than moxifloxacin, the gold standard, suggesting that may be promising therapy for bacterial
Abstract Background. Five neuroinvasive Bacillus cereus infections (4 fatal) occurred in hospitalized patients with acute myelogenous leukemia (AML) during a 9-month period, prompting an investigation by infection control and public health officials. Methods. Medical records of case-patients were reviewed matched case-control study was performed. Infection practices observed. Multiple environmental, food, medication samples common to AML cultured. Multilocus sequence typing performed for...
Bacillus cereus typically causes a self-limited foodborne gastrointestinal (GI) illness. Severe invasive infection occurs rarely, mainly among immunocompromised hosts. We describe cluster of B. infections 5 patients with acute myeloid leukemia and chemotherapy-induced neutropenia. The initial case presented occipital lobe abscess was found on biopsy to have organisms consistent species. Within 1 week, second patient died fulminant brain swelling hemorrhage. Neuropathologic autopsy culture...