A5020756463- Antibiotic Resistance in Bacteria
- Antibiotic Use and Resistance
- Bacterial Identification and Susceptibility Testing
- HIV/AIDS drug development and treatment
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- HIV Research and Treatment
- Hepatitis C virus research
- Microtubule and mitosis dynamics
- Cancer Treatment and Pharmacology
- Genomics and Phylogenetic Studies
- Biochemical and Molecular Research
- Cancer therapeutics and mechanisms
- Computational Drug Discovery Methods
- Systemic Lupus Erythematosus Research
- Viral gastroenteritis research and epidemiology
- Mosquito-borne diseases and control
- HIV/AIDS Research and Interventions
- Bacterial Genetics and Biotechnology
- 14-3-3 protein interactions
- Pneumonia and Respiratory Infections
- SARS-CoV-2 and COVID-19 Research
- Dengue and Mosquito Control Research
- Algal biology and biofuel production
- Click Chemistry and Applications
Centers for Disease Control and Prevention
2018-2025
National Center for Emerging and Zoonotic Infectious Diseases
2019-2025
Texas Department of State Health Services
2021-2022
Lubbock Christian University
2022
University Medical Center
2022
Utah Department of Health
2021
Washington State Department of Health
2021
Arizona Department of Health Services
2021
Arkansas Department of Health
2021
Oregon Health Authority
2021
Pseudomonas aeruginosa is intrinsically resistant to many antimicrobial drugs, making carbapenems crucial in clinical management. During July-October 2015 the United States, we piloted laboratory-based surveillance for carbapenem-resistant P. (CRPA) at sentinel facilities Georgia, New Mexico, Oregon, and Tennessee, population-based Monroe County, NY. An incident case was first isolate antipseudomonal from a patient 30-day period any source except nares, rectum or perirectal area, feces. We...
The evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in the emergence new variant lineages that have exacerbated COVID-19 pandemic. Some those variants were designated as concern/interest (VOC/VOI) by national or international authorities based on many factors including their potential impact vaccine-mediated protection from disease. To ascertain and rank risk VOCs VOIs, we analyze ability 14 (614G, Alpha, Beta, Gamma, Delta, Epsilon, Zeta, Eta, Theta,...
Abstract Background Carbapenemase-producing, carbapenem-resistant Pseudomonas aeruginosa (CP-CRPA) are extensively drug-resistant bacteria. We investigated the source of a multistate CP-CRPA outbreak. Methods Cases were defined as US patient's first isolation P. sequence type 1203 with carbapenemase gene blaVIM-80 and cephalosporinase blaGES-9 from any specimen collected reported to Centers for Disease Control Prevention during 1 January 2022–15 May 2023. conducted 1:1 matched case-control...
Zika virus is an emerging crisis as infection implicated in severe neurological disorders-Guillain-Barré syndrome and fetal microcephaly. There are currently no treatment options available for infection. This part of the flavivirus genus closely related to Dengue Fever Virus, West Nile Japanese Encephalitis Virus. Like other flaviviruses, genome encodes three structural proteins (capsid, precursor membrane, envelope) seven nonstructural (NS1, NS2A, NS2B, NS3, NS4A, NS4B, NS5). Currently,...
We report on a carbapenemase-producing hypervirulent Klebsiella pneumoniae (CP-hvKP) isolate collected from U.S. patient at an outpatient clinic. The was identified as K. serotype K1 sequence type 23 and included both hypervirulence (with rmpA, rmpA2 iroBCDN, peg-344, iucABCD-iutA genes) carbapenemase-encoding (blaKPC-2) plasmid. emergence of CP-hvKP underscores the importance clinical awareness this pathotype need for continued monitoring in United States.
Carbapenemase gene-positive (CP) Gram-negative bacilli are of significant clinical and public health concern. Their rapid detection containment critical to preventing their spread additional infections they can cause.
Abstract Background Plasmid-mediated outbreaks have been associated with gaps in infection control (IC) and environmental reservoirs such as sink drains. In October 2023, the Virginia Department of Health (VDH) identified a cluster four Klebsiella pneumoniae carbapenemase (KPC)-producing variicola urinary tract infections among patients at Acute Care Hospital A (ACH A). VDH CDC collaborated to investigate source prevent further transmission. Methods We reviewed medical records, directly...
Abstract In the United States, New Delhi metallo-beta-lactamase (NDM)-producing carbapenem-resistant Enterobacterales (CRE) are frequently associated with healthcare encounters. From September 2021 to 2022, 21 patients NDM-CRE identified from urine and without exposure were reported Centers for Disease Control Prevention. Isolates genetically similar healthcare-associated strains.
Symmetric, dimeric daclatasvir (BMS-790052) is the clinical lead for a class of picomolar inhibitors HCV replication. While specific, resistance-bearing mutations at positions 31 and 93 domain I strongly suggest viral NS5A as target, structural mechanism(s) drugs' activities resistance remains unclear. Several previous models suggested symmetric binding modes relative to homodimeric target; however, none can fully explain SAR details this class. We present semiautomated workflows model...
The CDC's Emerging Infections Program (EIP) conducted population- and laboratory-based surveillance of US carbapenem-resistant Pseudomonas aeruginosa (CRPA) from 2016 through 2018. To characterize the pathotype, 1,019 isolates collected this project underwent antimicrobial susceptibility testing whole-genome sequencing. Sequenced genomes were classified using seven-gene multilocus sequence typing (MLST) scheme a core genome (cg)MLST was used to determine phylogeny. Both chromosomal...
Abstract Objective The incidence of infections from extended-spectrum β-lactamase (ESBL)–producing Enterobacterales (ESBL-E) is increasing in the United States. We describe epidemiology ESBL-E at 5 Emerging Infections Program (EIP) sites. Methods During October–December 2017, we piloted active laboratory- and population-based (New York, New Mexico, Tennessee) or sentinel (Colorado, Georgia) surveillance. An incident case was first isolation normally sterile body sites urine Escherichia coli...
Abstract Reports of organisms harboring multiple carbapenemase genes have increased since 2010. During October 2012–April 2019, the Centers for Disease Control and Prevention documented 151 these isolates from 100 patients in United States. Possible risk factors included recent history international travel, inpatient healthcare, solid organ or bone marrow transplantation.
Pseudomonas aeruginosa is an opportunistic human pathogen that frequently causes health care-associated infections (HAIs). Due to its metabolic diversity and ability form biofilms, this Gram-negative nonfermenting bacterium can persist in the care environment, which lead prolonged HAI outbreaks. We describe creation of a core genome multilocus sequence typing (cgMLST) scheme provide stable platform for rapid comparison P. isolates using whole-genome sequencing (WGS) data.
Tools used to identify genes in microbial sequences using a reference database generally report matches as percent identity, which can be difficult interpret cases with <100% sequence changes specific amino acids have dramatic effects on protein function, such when they occur substrate binding regions or enzyme active sites, turn phenotypes like antimicrobial resistance virulence.Here, we present GAMMA, an open-source tool for Gene Allele Mutation Microbial Assessment, uses coding-level...
Infections caused by extended-spectrum β-lactamase (ESBL)-producing Escherichia coli are a significant cause of morbidity and health care costs. Globally, the prevailing clonal type is ST131 in association with bla CTX-M-15 gene.
ABSTRACT Ribonucleoside analog inhibitors (rNAI) target the hepatitis C virus (HCV) RNA-dependent RNA polymerase nonstructural protein 5B (NS5B) and cause chain termination. Here, we expand our studies on β- d -2′- -methyl-2,6-diaminopurine-ribonucleotide (DAPN) phosphoramidate prodrug 1 (PD1) as a novel investigational inhibitor of HCV. DAPN-PD1 is metabolized intracellularly into two distinct bioactive nucleoside triphosphate (TP) analogs. The first metabolite, 2′- -methyl-GTP,...
On November 1, 2016, a point prevalence survey was conducted at Chicago skilled nursing facility with ventilated residents to understand the of carbapenemase-producing organisms in health care facilities region. The identified 20 patients Verona integron-encoded metallo-beta-lactamase–producing carbapenem-resistant Pseudomonas aeruginosa.
Nucleoside analog inhibitors (NAIs) are an important class of antiviral agents. Although highly effective, some NAIs with activity against hepatitis C virus (HCV) can cause toxicity, presumably due to off-target inhibition host mitochondrial RNA polymerase (POLRMT). The in vitro nucleotide substrate specificity POLRMT was studied order explore structure-activity relationships that facilitate the identification nontoxic NAIs. These findings have implications for development all anti-RNA
During June 2017-November 2019, a total 36 patients with carbapenem-resistant Pseudomonas aeruginosa harboring Verona-integron-encoded metallo-β-lactamase were identified in city western Texas, USA. A faucet contaminated the organism, through environmental sampling, specialty care room was likely source for infection subset of patients.