A5109527006- Viral gastroenteritis research and epidemiology
- Influenza Virus Research Studies
- Animal Virus Infections Studies
- Animal Disease Management and Epidemiology
- Viral Infections and Vectors
- Probiotics and Fermented Foods
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Immunology Research
- Viral Infections and Outbreaks Research
- Virus-based gene therapy research
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Acute Ischemic Stroke Management
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Biopolymer Synthesis and Applications
- Pharmacological Effects of Natural Compounds
- Food Quality and Safety Studies
- Bacteriophages and microbial interactions
- Neurological Disorders and Treatments
- Herpesvirus Infections and Treatments
- Traditional Chinese Medicine Analysis
- Neurological Disease Mechanisms and Treatments
- COVID-19 epidemiological studies
Centers for Disease Control and Prevention
2019-2024
National Center for Immunization and Respiratory Diseases
2020-2024
Georgia State University
2012-2020
Oak Ridge Institute for Science and Education
2019
Oak Ridge Associated Universities
2019
Qingdao University
2015
Affiliated Hospital of Qingdao University
2015
Inner Mongolia Medical College Hospital
2009
Xianyang Normal University
2007
Capital Medical University
2007
The evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in the emergence new variant lineages that have exacerbated COVID-19 pandemic. Some those variants were designated as concern/interest (VOC/VOI) by national or international authorities based on many factors including their potential impact vaccine-mediated protection from disease. To ascertain and rank risk VOCs VOIs, we analyze ability 14 (614G, Alpha, Beta, Gamma, Delta, Epsilon, Zeta, Eta, Theta,...
Clade 2.3.4.4b highly pathogenic avian influenza (HPAI) A(H5N1) viruses that are responsible for devastating outbreaks in birds and mammals pose a potential threat to public health. Here, we evaluated their susceptibility antivirals. Of 1,015 sequences of HPAI collected the United States during 2022, eight (∼0.8%) had molecular marker drug resistance an FDA-approved antiviral: three adamantane-resistant (M2-V27A), four oseltamivir-resistant (NA-H275Y), one baloxavir-resistant (PA-I38T)....
I. Abstract After nearly a decade without reported human A/H5N1 infections, Cambodia faced sudden resurgence with 16 cases between February 2023 and August 2024, all caused by A/H5 clade 2.3.2.1c viruses. Fourteen involved novel reassortant virus gene segments from both 2.3.4.4b The emergence of this genotype underscores the persistent ongoing threat avian influenza in Southeast Asia. This study details timeline genomic epidemiology these infections related poultry outbreaks Cambodia.
The global spread of the highly pathogenic avian influenza (HPAI) A(H5N1) virus poses a serious pandemic threat, necessitating swift development effective vaccines. success messenger RNA (mRNA) vaccine technology in COVID-19 pandemic, marked by its rapid and scalability, demonstrates potential for addressing other infectious threats, such as HPAI A(H5N1). We therefore evaluated mRNA candidates targeting panzootic A(H5) clade 2.3.4.4b viruses, which have been shown to infect range mammalian...
Oas1b was previously identified as the product of Flv(r) allele that confers flavivirus-specific resistance to virus-induced disease in mice by an uncharacterized, RNase L-independent mechanism. To gain insights about mechanism which specifically reduces efficiency flavivirus replication, cellular protein interaction partners were and their involvement Oas1b-mediated analyzed. Initial difficulties getting two-hybrid assay work with full-length led discovery this Oas uniquely has a C-terminal...
During 2023-2024, highly pathogenic avian influenza A(H5N1) viruses from clade 2.3.2.1c caused human infections in Cambodia and 2.3.4.4b the Americas. We assessed susceptibility of those to approved investigational antiviral drugs. Except for 2 isolated Cambodia, all were susceptible M2 ion channel-blockers cell culture-based assays. In neuraminidase inhibition assay, displayed inhibitor drugs oseltamivir, zanamivir, peramivir, laninamivir, AV5080. Oseltamivir was ≈4-fold less potent at...
Significance All members of the order Nidovirales, including Simian hemorrhagic fever virus (SHFV), produce subgenomic mRNAs (sg mRNAs) for their 3′ genes regulated by genomic transcription regulatory sequences (TRSs). We used a next-generation sequencing–facilitated approach to comprehensively analyze nidovirus sg mRNA transcriptome. The discovery high redundancy individual and multiple previously unreported encoding nonstructural proteins, alternative reading frame or C-terminal peptides...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has evolved into numerous lineages with unique spike mutations and caused multiple epidemics domestically globally. Although COVID-19 vaccines are available, new variants the capacity for immune evasion continue to emerge. To understand characterize evolution of circulating SARS-CoV-2 in U.S., Centers Disease Control Prevention (CDC) initiated National Strain Surveillance (NS3) program received thousands clinical specimens from...
Influenza A(H5N1) viruses have been detected in US dairy cow herds since 2024. We assessed the pathogenesis, transmission, and airborne release of A/Michigan/90/2024, an H5N1 isolate from a farm worker Michigan, ferret model. Results show this virus caused transmission with moderate pathogenicity, including limited extrapulmonary spread, without lethality.
Low-pathogenicity avian influenza A(H9N2) viruses, enzootic in poultry populations Asia, are associated with fewer confirmed human infections but higher rates of seropositivity compared to A(H5) or A(H7) subtype viruses. Cocirculation and viruses leads the generation reassortant bearing internal genes markers mammalian adaptation, warranting continued surveillance both populations. Here, we describe active efforts live markets Vietnam 2018 compare representative G1 Y280 lineage that have...
Recently, a novel antiviral compound (K22) that inhibits replication of broad range animal and human coronaviruses was reported to interfere with viral RNA synthesis by impairing double-membrane vesicle (DMV) formation (Lundin et al., 2014). Here we assessed potential activities K22 against viruses representing two (sub)families the order Nidovirales, Arteriviridae (porcine reproductive respiratory syndrome virus [PRRSV], equine arteritis [EAV] simian hemorrhagic fever [SHFV]), Torovirinae...
The human OAS1 (hOAS1) gene produces multiple possible isoforms due to alternative splicing events and sequence variation among individuals, some of which affect splicing. unique C-terminal sequences the hOAS1 could differentially synthetase activity, protein stability, partner interactions and/or cellular localization. Recombinant p41, p42, p44, p46, p48, p49 p52 isoform proteins expressed in bacteria were each able synthesize trimer higher order 2′-5′ linked oligoadenylates vitro response...
ABSTRACT The N-terminal region of simian hemorrhagic fever virus (SHFV) nonstructural polyprotein 1a is predicted to encode three papain-like proteases (PLP1α, PLP1β, and PLP1γ). Catalytic residues cleavage sites for each the SHFV PLP1s were by alignment PLP1 sequences with other as well those arteriviruses, catalytic shown be proximal homology modeling nsp1s on porcine respiratory reproductive syndrome (PRRSV) nsp1 crystal structures. functionality Cys was tested analysis autoproteolytic...
Introductory The evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in the emergence many new variant lineages that have exacerbated COVID-19 pandemic. Some those variants were designated as concern/interest (VOC/VOI) by national or international authorities based on factors including their potential impact vaccines. To ascertain and rank risk VOCs VOIs, we analyzed ability to escape from vaccine-induced antibodies. showed differential reductions...
To explore the protective effects of Danhong injection against cerebral damage during on-pump coronary artery bypass graft surgery and its mechanism. Methods . Fifty patients scheduled for CABG were randomly divided into group (group D) control C). Group D was given while C same volume normal saline when cut open. Jugular bulb blood right before operation began (T1), body temperature rewarming to 36°C (T2), 30 min after termination cardiopulmonary (T3), 6 hrs CPB (T4) collected. The...
Since 2013, a total of 167 human infections with swine-origin (variant) influenza A viruses A(H1N1)v, A(H1N2)v, and A(H3N2)v subtypes have been reported in the United States. Analysis 147 genome sequences revealed that nearly all had S31N substitution, an M2 channel blocker-resistance marker, whereas neuraminidase inhibitor-resistance markers were not found. Two polymerase acidic substitution (I38M or E199G) associated decreased susceptibility to baloxavir, inhibitor viral cap-dependent...