A5043655803- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Malaria Research and Control
- Zoonotic diseases and public health
- COVID-19 epidemiological studies
- Viral Infections and Outbreaks Research
- SARS-CoV-2 and COVID-19 Research
- Virology and Viral Diseases
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Vector-borne infectious diseases
- Dengue and Mosquito Control Research
- Animal Disease Management and Epidemiology
- Insect symbiosis and bacterial influences
- Viral Infections and Immunology Research
- Rabies epidemiology and control
- Influenza Virus Research Studies
- Respiratory viral infections research
- Parasites and Host Interactions
- Parasite Biology and Host Interactions
- Microbial infections and disease research
- Herpesvirus Infections and Treatments
- Bat Biology and Ecology Studies
- COVID-19 Clinical Research Studies
- Data-Driven Disease Surveillance
Institut Pasteur du Cambodge
2016-2025
The University of Texas at El Paso
2023
Institut Pasteur de Nouvelle Calédonie
2021
Cambodia National Malaria Center
2016
Institut Pasteur
2010-2015
Johns Hopkins University
2015
Ministry of Health
2015
Centre d’Infectiologie Christophe Mérieux of Laos
2005-2009
Significance Our work provides evidence that people who are infected with dengue virus without developing detectable clinical symptoms or prior to the onset of infectious mosquitoes. At a given level viremia, symptom-free were markedly more mosquitoes than clinically symptomatic patients. results fundamentally change current paradigm for epidemiology and control, based on detection virus-infected cases apparent illness.
The death toll and economic loss resulting from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic are stark reminders that we vulnerable to zoonotic viral threats. Strategies needed identify characterize animal viruses pose greatest risk of spillover spread in humans inform public health interventions. Using expert opinion scientific evidence, identified host, viral, environmental factors contributing virus humans. We then developed a ranking framework interactive web...
Abstract Knowledge of the origin and reservoir coronavirus responsible for ongoing COVID-19 pandemic is still fragmentary. To date, closest relatives to SARS-CoV-2 have been detected in Rhinolophus bats sampled Yunnan province, China. Here we describe identification related coronaviruses two shameli Cambodia 2010. Metagenomic sequencing identifies nearly identical viruses sharing 92.6% nucleotide identity with SARS-CoV-2. Most genomic regions are closely SARS-CoV-2, exception a region spike,...
IgG fucosylation predicts dengue severity Secondary infections with virus (DENV) can produce life-threatening symptoms, including thrombocytopenia and hemorrhagic disease, when preexisting DENV-reactive immunoglobulin G1 (IgG1) antibodies promote the infection of immune cells. Although severe symptoms are associated increased levels afucosylated IgG1 glycoforms, it is unclear whether this simply a result or if phenomenon that dictate susceptibility to disease. Bournazos et al. studied Fab Fc...
A better description of the extent and structure genetic diversity in dengue virus (DENV) endemic settings is central to its eventual control. To this end we determined complete coding region sequence 187 DENV-2 genomes 68 E genes from viruses sampled Vietnamese patients between 1995 2009. Strikingly, an episode genotype replacement was observed, with Asian 1 lineage entirely displacing previously dominant Asian/American viruses. This event also seems have occurred within Thailand Cambodia,...
Despite estimates that, each year, as many 300 million dengue virus (DENV) infections result in either no perceptible symptoms (asymptomatic) or that are sufficiently mild to go undetected by surveillance systems (inapparent), it has been assumed these contribute little onward transmission. However, recent blood-feeding experiments with Aedes aegypti mosquitoes showed people asymptomatic and pre-symptomatic DENV capable of infecting mosquitoes. To place those findings into context, we used...
Background Detection of dengue NS1 antigen in acute infection has been proposed for early diagnosis disease. The aim this study was to evaluate the clinical and virological factors influencing performance Platelia Ag kit (BioRad) assess potential use viral loads as markers disease severity. Methodology/Principal Findings Blood specimens were collected from patients hospitalized at Kampong Cham hospital during 2006 2007 epidemics Cambodia. Dengue confirmed 243/339 symptomatic 17 asymptomatic...
Dengue laboratory diagnosis is essentially based on detection of the virus, its components or antibodies directed against virus in blood samples. Blood, however, may be difficult to draw some patients, especially children, and sampling during outbreak investigations epidemiological studies face logistical challenges limited compliance invasive procedures from subjects. The aim this study was assess possibility using saliva urine samples instead for dengue diagnosis.Serial plasma, were...
The drivers and patterns of zoonotic virus emergence in the human population are poorly understood. mosquito Aedes aegypti is a major arbovirus vector native to Africa that invaded most world's tropical belt over past four centuries, after evolution "domestic" form specialized biting humans breeding water storage containers. Here, we show specialization subsequent spread A. out were accompanied by an increase its intrinsic ability acquire transmit emerging pathogen Zika virus. Thus, recent...
Jingmenvirus is a recently identified group of segmented RNA viruses phylogenetically linked with unsegmented Flaviviridae viruses. Primarily in various tick genera originating China, geographical distribution has rapidly expanded to cover Africa, South America, Caribbean, and Europe. The identification Jingmen-related mammals, including febrile humans, opens the possibility that Jingmenviruses may be novel tick-borne arboviruses. In this study, we aimed at increasing knowledge host range,...
Abstract Knowledge of the origin and reservoir coronavirus responsible for ongoing COVID-19 pandemic is still fragmentary. To date, closest relatives to SARS-CoV-2 have been detected in Rhinolophus bats sampled Yunnan province, China. Here we describe identification related coronaviruses two shameli Cambodia 2010. Metagenomic sequencing identified nearly identical viruses sharing 92.6% nucleotide identity with SARS-CoV-2. Most genomic regions are closely SARS-CoV-2, exception a small region...
As the largest group of mammalian species, which are also widely distributed all over world, rodents natural reservoirs for many diverse zoonotic viruses. A comprehensive understanding core virome should therefore assist in efforts to reduce risk future emergence or re-emergence rodent-borne pathogens.
Infection with one of dengue viruses 1 to 4 (DENV1-4) induces protective antibodies against homotypic infection. However, a notable feature is the ability use preexisting heterotypic infect Fcγ receptor–bearing immune cells, leading higher viral load and immunopathological events that augment disease. We tracked antigenic dynamics each DENV serotype by using 1944 sequenced isolates from Bangkok, Thailand, between 1994 2014 (348 strains), in comparison regional global diversity (64 strains)....
Arthropod-borne viruses (arboviruses) are transmitted by arthropod vectors to humans, leading disease outbreaks mainly in tropical regions of the world.1Weaver SC Charlier C Vasilakis N Lecuit M Zika, chikungunya, and other emerging vector-borne viral diseases.Annu Rev Med. 2018; 69: 395-408Crossref PubMed Scopus (234) Google Scholar Although many arboviruses known, three Aedes-spp-borne particularly concerning for humans at a global scale due recurring large expanding epidemic dengue virus,...
Since SARS-CoV-2's emergence, studies in Southeast Asia, including Cambodia, have identified related coronaviruses (CoVs) rhinolophid bats. This pilot study investigates the prevalence and diversity of CoVs wildlife from two Cambodian provinces known for trade environmental changes, factors favoring zoonotic spillover risk. Samples were collected 2020 to 2022 using active (capture swabbing bats rodents) non-invasive (collection feces bat caves habitats) methods. RNA was screened conventional...
Background Dengue vaccines are now in late-stage development, and evaluation robust estimates of dengue disease burden needed to facilitate further development introduction. In Cambodia, the national case-definition only allows reporting children less than 16 years age, little is known about rural areas among older persons. To estimate true largest province Kampong Cham, we conducted community-based active fever surveillance 0-to-19–year age group villages urban during 2006–2008. Methods...
Dengue has been reportable in Cambodia since 1980. Virological surveillance began 2000 and sentinel was established at six hospitals 2001. Currently, national comprises passive active data collection reporting on hospitalized children aged 0-15 years. This report summarizes collected 1980.Crude for 1980-2001 are presented, while from 2002-2008 used to describe disease trends the effect of vector control interventions. Trends dengue incidence were analysed using Prais-Winsten generalized...
Background Dengue diagnosis is complex and until recently only specialized laboratories were able to definitively confirm dengue infection. Rapid tests are now available commercially making biological possible in the field. The aim of this study was evaluate a combined rapid test for detection NS1 IgM/IgG antibodies. evaluation made prospectively field conditions included impact its use as point-of-care case management well retrospectively against panel well-characterized samples reference...
Host-associated microbes, collectively known as the microbiota, play an important role in biology of multicellular organisms. In mosquito vectors human pathogens, gut bacterial microbiota influences vectorial capacity and has become subject intense study. laboratory studies vector biology, genetic effects are often inferred from differences between geographically genetically diverse colonies mosquitoes that reared same insectary. It is unclear, however, to what extent can be confounded by...
Dengue is the most prevalent arthropod-borne viral disease. Clinical symptoms of dengue virus (DENV) infection range from classical mild fever to severe, life-threatening shock syndrome. However, DENV infections cause few or no symptoms. Asymptomatic DENV-infected patients provide a unique opportunity decipher host immune responses leading elimination without negative impact on t v 'health. We used an integrated approach transcriptional profiling and immunological analysis comparing...