A5100412676- Plant Virus Research Studies
- Viral Infections and Vectors
- Viral gastroenteritis research and epidemiology
- Cancer-related molecular mechanisms research
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- MicroRNA in disease regulation
- RNA modifications and cancer
- Genomics and Phylogenetic Studies
- Hematopoietic Stem Cell Transplantation
- RNA and protein synthesis mechanisms
- Plant and Fungal Interactions Research
- Mosquito-borne diseases and control
- Plant tissue culture and regeneration
- Gene expression and cancer classification
- Transgenic Plants and Applications
- Animal Disease Management and Epidemiology
- Cancer Genomics and Diagnostics
- Immune Cell Function and Interaction
- Extracellular vesicles in disease
- Metallurgical Processes and Thermodynamics
- Virus-based gene therapy research
- T-cell and B-cell Immunology
- Cytomegalovirus and herpesvirus research
- Genomic variations and chromosomal abnormalities
China Medical University
2014-2025
First Hospital of China Medical University
2005-2025
Xiangtan Electric Manufacturing Group (China)
2023-2025
University of Southern California
2016-2024
Xi'an Technological University
2016-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2014-2024
Broad Center
2018-2024
Tianjin Medical University Cancer Institute and Hospital
2023-2024
Sichuan University
2024
Shanghai Jiao Tong University
2022-2024
From Genome to Regulatory Networks For biologists, having a genome in hand is only the beginning—much more investigation still needed characterize how used help produce functional organism (see Perspective by Blaxter ). In this vein, Gerstein et al. (p. 1775 ) summarize for Caenorhabditis elegans genome, and The modENCODE Consortium 1787 Drosophila melanogaster full transcriptome analyses over developmental stages, genome-wide identification of transcription factor binding sites,...
We evaluated 25 protocol variants of 14 independent computational methods for exon identification, transcript reconstruction and expression-level quantification from RNA-seq data. Our results show that most algorithms are able to identify discrete components with high success rates but assembly complete isoform structures poses a major challenge even when all constituent elements identified. Expression-level estimates also varied widely across methods, based on similar models. Consequently,...
ABSTRACT Bats are natural hosts for a large variety of zoonotic viruses. This study aimed to describe the range bat viromes, including viruses from mammals, insects, fungi, plants, and phages, in 11 insectivorous species (216 bats total) common six provinces China. To analyze we used sequence-independent PCR amplification next-generation sequencing technology (Solexa Genome Analyzer II; Illumina). The viromes were identified by sequence similarity comparisons known mammalian included those...
Rodents represent around 43% of all mammalian species, are widely distributed, and the natural reservoirs a diverse group zoonotic viruses, including hantaviruses, Lassa tick-borne encephalitis viruses. Thus, analyzing viral diversity harbored by rodents could assist efforts to predict reduce risk future emergence diseases.We used next-generation sequencing metagenomic analysis survey for range families in other small animals orders Rodentia, Lagomorpha, Soricomorpha China. We sampled 3,055...
ABSTRACT Viral infectious diseases represent a major threat to public health and are among the greatest disease burdens worldwide. Rapid accurate identification of viral agents is crucial for both outbreak control estimating regional burdens. Recently developed metagenomic methods have proven be powerful tools simultaneous pathogen detection. Here, we performed systematic study capability short-read-based approach in molecular detection pathogens nasopharyngeal aspirate samples from patients...
The revolution in DNA sequencing technologies has now made it feasible to determine the genome sequences of many individuals; i.e., “personal genomes.” Genome cells and tissues from both normal disease states have been determined. Using current approaches, whole human are not typically assembled determined de novo, but, instead, variations relative a reference sequence identified. We discuss state personal sequencing, main steps involved determining (i.e., identifying single-nucleotide...
Abstract Background In China, a rapid expansion of Hand, foot, and mouth disease (HFMD) outbreaks has occurred since 2004 HFMD become an important issue for China. However, people are still only concerned with human enterovirus 71(HEV-71) coxsackie virus A16 (CV-A16). Much what is known about the other infections relies on fractional evidence old epidemic data, little knowledge concerning their distribution. To alert potential threatens enteroviruses, our study genetically characterized...
To clarify the evolutionary relationships among betavoronaviruses that infect bats, we analyzed samples collected during 2010-2011 from 14 insectivorous bat species in China. We identified complete genomes of 2 novel betacoronaviruses Rhinolophus pusillus and Chaerephon plicata which showed close genetic with severe acute respiratory syndrome coronaviruses.
Significance Hematopoietic stem cells (HSCs) are the key therapeutic component of bone marrow transplantation, first and most prevalent clinical cell therapy. HSCs sustain daily life-long blood immune production through a complex stepwise lineage commitment process. In this work, we analyzed HSC at clonal level identified regulatory mechanisms that undetectable by conventional population-level studies. We uncovered distinct pathways lead to differential imbalances. Furthermore, showed...
Abstract Background Mesenchymal stem cells (MSCs) are pluripotent mesenchymal present in various adult tissues. MSCs secrete exosomes as regulators of the tumor niche, with involvement tumorigenesis and metastasis. The regulatory role microRNAs (miRs or miRNAs) via targeting cyclin E1 (CCNE1) E2 (CCNE2) has been extensively reported. Since considered protective enriched sources shuttle miRNAs, we hypothesized that exosomal transfer miR-144 from bone marrow-derived (BMMSCs) would affect...
As the largest group of mammalian species, which are also widely distributed all over world, rodents natural reservoirs for many diverse zoonotic viruses. A comprehensive understanding core virome should therefore assist in efforts to reduce risk future emergence or re-emergence rodent-borne pathogens.
A critical problem in biology is understanding how cells choose between self-renewal and differentiation. To generate a comprehensive view of the mechanisms controlling early hematopoietic precursor differentiation, we used systems-based approaches murine EML multipotential as primary model. give rise to mixture self-renewing Lin-SCA+CD34+ partially differentiated non-renewing Lin-SCA-CD34− cell autonomous fashion. We identified validated HMG box protein TCF7 regulator this...
Background New therapeutic tools and molecular targets are needed for treatment of Japanese encephalitis virus (JEV) infections. JEV requires an α-1 translational frameshift to synthesize the NS1' protein required viral neuroinvasiveness. Several flavonoids have been shown possess antiviral activity in vitro against a wide spectrum viruses. To date, activities flavonol kaempferol (Kae) isoflavonoid daidzin (Dai) not described. Methodology/Principal Findings The 50% cytotoxic concentration...
Outbreaks of severe acute respiratory syndrome (SARS) in 2002, Middle East 2012 and fatal swine diarrhea 2017 caused serious infectious diseases humans livestock, resulting public health threats huge economic losses. All such coronaviruses (CoVs) were confirmed to originate from bats. To continuously monitor the epidemic-related CoVs bats, virome analysis was used classify 831 bats 15 species Yunnan, Guangxi, Sichuan Provinces between August 2016 May 2017. We identified 11 CoV strains 22...
Enterovirus 71 (EV71) can cause severe disease and even lead to death in children, an effective antiviral drug is currently unavailable. The anti-EV71 effect of chrysin (5,7-dihydroxyflavone), a natural flavonoid commonly found many plants, was tested this report. By using the predicting program Autodock 4.0 vitro protease inhibition assay, we that could suppress viral 3Cpro activity. Replication RNA production capsid protein infectious virion were strongly inhibited by chrysin, without...
Hypoxia-exposed lung cancer-released exosomal microRNA-23a (miR-23a) has been shown to enhance angiogenesis as well vascular permeability, contributing the close correlation between miR-23a and tumorigenesis. The current study aimed investigate whether gastric cancer (GC) cell-derived could induce elucidate potential mechanisms associated with process. Differentially expressed miRNAs in GC were initially screened from Gene Expression Omnibus database. Target genes selected following...
Copy-number variants (CNVs) are an abundant form of genetic variation in humans. However, approaches for determining exact CNV breakpoint sequences (physical deletion or duplication boundaries) across individuals, crucial associating genotype to phenotype, have been lacking so far, and the vast majority CNVs reported with approximate genomic coordinates only. Here, we report approach, called BreakPtr, fine-mapping (available from http://breakptr.gersteinlab.org). We statistically integrate...
Recent studies have demonstrated the genetic significance of insertions, deletions, and other more complex structural variants (SVs) in human population. With development next-generation sequencing technologies, high-throughput surveys SVs on whole-genome level become possible. Here we present split-read identification, calibrated (SRiC), a sequence-based method for SV detection. We start by mapping each read to reference genome standard fashion using gapped alignment. Then identify SVs,...
Tobacco curly shoot virus (TbCSV) is a monopartite begomovirus associated with betasatellite (Tobacco betasatellite, TbCSB), which causes serious leaf curl disease on tomato and tobacco in China. It interesting that TbCSV induced severe upward curling Nicotiana benthamiana, but the presence of TbCSB, symptoms changed to be downward curling. However, mechanism interactions between viral pathogenicity, host defense, viral-betasatellite virus-host remains unclear.In this study, RNA-seq was used...