A5102842358- Malaria Research and Control
- Mosquito-borne diseases and control
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- Parasites and Host Interactions
- vaccines and immunoinformatics approaches
- Vector-borne infectious diseases
- Long-Term Effects of COVID-19
- Invertebrate Immune Response Mechanisms
- SARS-CoV-2 detection and testing
- COVID-19 epidemiological studies
- Algorithms and Data Compression
- Complement system in diseases
- Antifungal resistance and susceptibility
- Computational Drug Discovery Methods
- Aquaculture disease management and microbiota
- Healthcare Systems and Technology
- Heparin-Induced Thrombocytopenia and Thrombosis
- Chronic Myeloid Leukemia Treatments
- Cardiac Ischemia and Reperfusion
- Viral Infections and Outbreaks Research
- Immune responses and vaccinations
- COVID-19 and healthcare impacts
- Plant Virus Research Studies
CDC Foundation
2025
National Center for Emerging and Zoonotic Infectious Diseases
2025
Epidemic Intelligence Service
2025
Centers for Disease Control and Prevention
2018-2023
Division of Parasitic Diseases and Malaria
2018-2023
Center for Global Health
2020-2023
Noah's Ark Children's Hospital for Wales
2023
Universidad de Londres
2023
Texas Christian University
2023
The Centers
2022
Infection with Plasmodium can elicit antibodies that inhibit parasite survival in the mosquito, when they are ingested an infectious blood meal. Here, we determine transmission-reducing activity (TRA) of naturally acquired from 648 malaria-exposed individuals using lab-based mosquito-feeding assays. Transmission inhibition is significantly associated antibody responses to Pfs48/45, Pfs230, and 43 novel gametocyte proteins assessed by protein microarray. In field-based assays likelihood rate...
Detection of Plasmodium antigens provides evidence malaria infection status and is the basis for most diagnosis.
Background Without an effective vaccine, as was the case early in 2014–2016 Ebola Outbreak West Africa, disease control depends entirely on interrupting transmission through detection and prompt patient isolation. Lateral Flow Immunoassays (LFI) are a potential supplement to centralized reference laboratory testing for diagnosis of Virus Disease (EVD). The goal this study assess performance commercially available simple rapid antigen LFIs, submitted review WHO via Emergency Use Assessment...
Assessment of exposure to malaria vectors is important our understanding spatial and temporal variations in disease transmission facilitates the targeting evaluation control efforts. Recently, an immunogenic Anopheles gambiae salivary protein (gSG6) was identified proposed as basis immuno-assay determining Afrotropical vectors. In present study, IgG responses gSG6 6 antigens (CSP, AMA-1, MSP-1, MSP-3, GLURP R1, R2) were compared incidence a cohort children from Korogwe district, Tanzania,...
Deletions of pfhrp2 and paralogue pfhrp3 (pfhrp2/3) genes threaten Plasmodium falciparum diagnosis by rapid diagnostic test. We examined 1,002 samples from suspected malaria patients in Djibouti City, Djibouti, to investigate pfhrp2/3 deletions. performed assays for antigen carriage, genotyping, sequencing 7 neutral microsatellites assess relatedness. By PCR assay, 311 (31.0%) tested positive P. infection, 296 (95.2%) were successfully genotyped; 37 (12.5%) pfhrp2+/pfhrp3+, 51 (17.2%)...
Abstract Background Candida auris (C. auris) is an emerging healthcare-associated fungal pathogen associated with high mortality and antifungal resistance. Targeted screening based on patient risk factors facility type important for identifying colonized patients implementing recommended infection prevention control (IPC) precautions. Understanding the epidemiology of C. who subsequently have positive clinical specimens (screening-to-clinical (STC)) can help us understand course impact...
Abstract In the United States, New Delhi metallo-beta-lactamase (NDM)-producing carbapenem-resistant Enterobacterales (CRE) are frequently associated with healthcare encounters. From September 2021 to 2022, 21 patients NDM-CRE identified from urine and without exposure were reported Centers for Disease Control Prevention. Isolates genetically similar healthcare-associated strains.
Abstract Background Accurate sampling of sub-microscopic gametocytes is necessary for epidemiological studies to identify the infectious reservoir Plasmodium falciparum . Detection gametocyte mRNA achieves sensitive detection, but requires careful handling samples. Filter papers can be used collecting RNA samples, rigorous testing their capacity withstand adverse storage conditions has not been fully explored. Methods Three dilutions: 10/μL, 1.0/μL and 0.1/μL were spotted onto Whatman™ 903...
Infections with helminths and Plasmodium spp. overlap in their geographical distribution. It has been postulated that helminth infections may influence malarial transmission by altering falciparum gametocytogenesis. This cross-sectional study assessed the effect of on P. gametocyte carriage humoral immune responses to sexual stage antigens Gabon. Schistosoma haematobium filarial as well asexual forms were determined. The antibody measured (Pfs230, Pfs48/45) (AMA1, MSP1, GLURP). A total 287...
Abstract Background Histidine-rich protein 2 (HRP2)-based malaria rapid diagnostic tests (RDTs) are effective and widely used for the detection of wild-type Plasmodium falciparum infections. Although recent studies have reported false negative HRP2 RDT results due to pfhrp2 pfhrp3 gene deletions in different countries, there is a paucity data on these genes Tanzania. Methods A community-based cross-sectional survey was conducted between July November 2017 four regions: Geita, Kigoma, Mtwara...
Asymptomatic malaria infections can serve as potential reservoirs for transmission. The density of parasites contained in these range from microscopic to submicroscopic densities, making the accurate detection asymptomatic parasite carriage highly dependent on sensitivity tools used diagnosis. This study sought evaluate sensitivities a variety molecular and serological diagnostic at determining prevalence Plasmodium falciparum two communities with varying prevalence.Whole blood was collected...
Histidine-rich protein 2 (HRP2) detecting rapid diagnostic tests (RDTs) have played an important role in enabling prompt malaria diagnosis remote locations. However, emergence of pfhrp2 deleted parasites is threatening the efficacy RDTs, and World Health Organization (WHO) has highlighted surveillance these deletions as a priority. Nested PCR used to confirm deletion but costly laborious. Due spurious amplification paralogue pfhrp3, identity nested exon 1 product must be confirmed by...
The Plasmodium falciparum parasite is the only human malaria that produces histidine-rich protein 2 and 3 (HRP2/3) antigens. Currently, HRP2/3 are widely used in rapid diagnostic tests (RDTs), but several global reports have recently emerged showing genetic deletion of one or both these antigens parasites. Deletion could pose a major concern for P. diagnosis Haiti which currently uses RDTs based solely on detection antigens.From September 2012 through February 2014, dried blood spots (DBS)...
Abstract The prevalence of Plasmodium falciparum hrp2 ( pfhrp2 )-deleted parasites threatens the efficacy most used and sensitive malaria rapid diagnostic tests highlights need for continued surveillance this gene deletion. While PCR methods are adequate determining presence or absence, they offer a limited view its genetic diversity. Here, we present portable sequencing method using MinION. Pfhrp2 amplicons were generated from individual samples, barcoded, pooled sequencing. To overcome...
Background: Burkholderia multivorans are gram-negative bacteria typically found in water and soil. B. outbreaks among patients without cystic fibrosis have been associated with exposure to contaminated medical devices or nonsterile aqueous products. Acquisition can also occur from environmental reservoirs like sinks other hospital sources. We describe an outbreak of hospitalized at 2 hospitals within the same healthcare system California (hospitals A B) between August 2021 July 2022....
Background: Candida auris is a frequently drug-resistant yeast that can cause invasive disease and easily transmitted in healthcare settings. Pediatric cases are rare the United States, with <10 reported before 2022. In August 2021, first C. case Las Vegas was identified an adult. By May 2022, 117 were across 16 facilities, including 3 pediatric at acute-care hospital (ACH) adult cases, representing cluster States. The CDC Nevada Division of Public Behavioral Health (NVDPBH) sought to...
Background: Despite recent reports of false negative results among histidine-rich protein 2 (HRP2) based-malaria rapid diagnostic tests (mRDTs) caused by pfhrp2/3 gene deletions in different countries, there is paucity data Tanzania. Methods: This study assessed the status 7,543 blood sample using laboratory multiplex antigen detection (Plasmodium lactate dehydrogenase - pLDH, aldolase, and HRP2). Samples showing mRDT negativity or aberrant relationship HRP2 to pan-Plasmodium antigens were...