A5036352618- Malaria Research and Control
- Mosquito-borne diseases and control
- Invertebrate Immune Response Mechanisms
- Insect symbiosis and bacterial influences
- Insect Resistance and Genetics
- Neurobiology and Insect Physiology Research
- Research on Leishmaniasis Studies
- Parasite Biology and Host Interactions
- Computational Drug Discovery Methods
- Trypanosoma species research and implications
- Insect Pest Control Strategies
- Viral Infections and Vectors
- Vector-borne infectious diseases
- Antimicrobial Peptides and Activities
- Hemoglobinopathies and Related Disorders
- Insect-Plant Interactions and Control
- Drug-Induced Hepatotoxicity and Protection
- Vibrio bacteria research studies
- Vector-Borne Animal Diseases
- Parasites and Host Interactions
- Iron Metabolism and Disorders
- Insect behavior and control techniques
- Physiological and biochemical adaptations
- vaccines and immunoinformatics approaches
- Nuclear and radioactivity studies
Université de Montpellier
2017-2024
Institut de Recherche pour le Développement
2015-2024
Centre National de la Recherche Scientifique
2014-2024
Agropolis International
2008-2024
Maladies Infectieuses et Vecteurs: Écologie, Génétique, Évolution et Contrôle
2014-2024
Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale
2011-2021
Centre Pasteur du Cameroun
2000-2021
Institut de Recherche pour le Développement
2006-2015
Institut de Recherche Agricole pour le Développement
2012-2013
Institut Pasteur
2007
Recent gains in reducing the global burden of malaria are threatened by emergence Plasmodium falciparum resistance to artemisinins. The discovery that mutations portions a P. gene encoding kelch (K13)–propeller domains major determinant has provided opportunities for monitoring such on scale.
The susceptibility of Anopheles mosquitoes to Plasmodium infections relies on complex interactions between the insect vector and malaria parasite. A number studies have shown that mosquito innate immune responses play an important role in controlling infection strength parasite clearance is under genetic control, but little known about influence environmental factors transmission success. We present here evidence composition gut microbiota one major components determine outcome infections....
Recognition of peptidoglycan (PGN) is paramount for insect antibacterial defenses. In the fruit fly Drosophila melanogaster, transmembrane PGN Protein LC (PGRP-LC) a receptor Imd signaling pathway that activated after infection with bacteria, mainly Gram-negative (Gram−). Here we demonstrate bacterial infections malaria mosquito Anopheles gambiae are sensed by orthologous PGRPLC protein which then activates involves Rel/NF-κB transcription factor REL2. leads to transcriptional induction...
Abstract Background Parasite concentration methods facilitate molecular, biochemical and immunological research on the erythrocytic stages of Plasmodium . In this paper, an adaptation magnetic MACS ® columns for purification human species is presented. This method was useful concentration/purification either schizonts or gametocytes. Results conclusions The removal non-parasitized red blood cells (in vivo in vitro) using (MACS) evaluated. easy-to-use technique enriched gametocytes from...
Introduction In the era of malaria elimination and eradication, drug-based vaccine-based approaches to reduce transmission are receiving greater attention. Such interventions require assays that reliably measure Plasmodium from humans Anopheles mosquitoes. Methods We compared two commonly used mosquito feeding assay procedures: direct skin membrane assays. Three conditions under which performed were examined: with i) whole blood, ii) blood pellets resuspended autologous plasma gametocyte...
Understanding the importance of gametocyte density on human-to-mosquito transmission is immediate relevance to malaria control. Previous work (Churcher et al., 2013) indicated a complex relationship between and mosquito infection. Here we use data from 148 feeding experiments naturally infected carriers show that much simpler depends both female male parasite density. The proportion mosquitoes primarily determined by gametocytes though low densities may be impeded lack parasites. Improved...
Infection with Plasmodium can elicit antibodies that inhibit parasite survival in the mosquito, when they are ingested an infectious blood meal. Here, we determine transmission-reducing activity (TRA) of naturally acquired from 648 malaria-exposed individuals using lab-based mosquito-feeding assays. Transmission inhibition is significantly associated antibody responses to Pfs48/45, Pfs230, and 43 novel gametocyte proteins assessed by protein microarray. In field-based assays likelihood rate...
The Anopheles midgut hosts diverse bacterial communities and represents a complex ecosystem. Several evidences indicate that mosquito microbiota interferes with malaria parasite transmission. However, the composition of salivary glands ovaries, two other biologically important tissues, has not been described so far. In this study, we investigated dynamics in tissues from emerging mosquitoes until 8 days after blood meal containing Plasmodium falciparum gametocytes temporal colonization...
We report the first finding of knockdown Leu-Phe and Leu-Ser mutations associated with resistance to pyrethroids DDT insecticides in malaria mosquito Anopheles gambiae from Cameroon. The mutation was found both M S molecular forms An. . Importantly, two specimens form were carry a heterozygous state.
Genetically controlled resistance of Anopheles gambiae mosquitoes to Plasmodium falciparum is a common trait in the natural population, and cluster loci were mapped Plasmodium-Resistance Island (PRI) A. genome. The APL1 family leucine-rich repeat (LRR) proteins was highlighted by candidate gene studies PRI, comprised paralogs APL1A, APL1B APL1C that share > or =50% amino acid identity. Here, we present functional analysis joint response members during mosquito infection with human rodent...
In much of sub-Saharan Africa, the mosquito Anopheles gambiae is main vector major human malaria parasite, Plasmodium falciparum. Convenient laboratory studies have identified genes that affect positively or negatively developmental cycle model rodent P. berghei. Here, we use transcription profiling and reverse genetics to explore whether five disparate gene regulators berghei development are also pertinent A. gambiae/P. falciparum interactions in semi-natural conditions, using field...
Many genes involved in the immune response of Anopheles gambiae, main malaria vector Africa, have been identified, but whether naturally occurring polymorphisms these underlie variation resistance to human parasite, Plasmodium falciparum, is currently unknown. Here we carried out a candidate gene association study identify single nucleotide (SNPs) associated with natural P. falciparum. A. gambiae M form mosquitoes from Cameroon were experimentally challenged three local wild falciparum...
To determine, 6 years after the adoption of intermittent preventive treatment pregnant women with sulfadoxine/pyrimethamine (IPTp-SP) in Cameroon, (i) polymorphism and prevalence Plasmodium falciparum dihydrofolate reductase (pfdhfr) dihydropteroate synthase (pfdhps) gene mutations associated resistance (ii) consequences use selection pfdhfr/pfdhps alleles. pfdhfr pfdhps genes from P. isolates collected Yaoundé (Cameroon) symptomatic malaria before taking IPTp-SP [SP− group (control) (n =...
The development of Plasmodium falciparum within the Anopheles gambiae mosquito relies on complex vector-parasite interactions, however resident midgut microbiota also plays an important role in mediating parasite infection. In natural conditions, microbial flora is diverse, composed commensal and symbiotic bacteria. We report here isolation culturable bacteria from mosquitoes collected field Cameroon their identification based 16S rRNA gene sequencing. next measured effect selected bacterial...
Malaria transmission-blocking vaccines (TBVs) represent a promising approach for the elimination and eradication of this disease. AnAPN1 is lead TBV candidate that targets surface antigen on midgut obligate vector Plasmodium parasite, Anopheles mosquito. In study, we demonstrated antibodies targeting block transmission falciparum vivax across distantly related anopheline species in countries to which malaria endemic. Using biochemical immunological approach, determined mechanism action...
Plasmodium falciparum is the causative agent of malaria, a disease that kills almost one million persons each year, mainly in sub-Saharan Africa. P. transmitted to human host by bite an Anopheles female mosquito, and gambiae sensus stricto most tremendous malaria vector Africa, widespread throughout afro-tropical belt. An. s.s. subdivided into two distinct molecular forms, namely M S forms. The forms are morphologically identical but they genetically, differ their distribution ecological...
A noninvasive malaria rapid test assesses subclinical carriage of Plasmodium falciparum (human malaria) parasites at the point need.
Serpins are central to the modulation of various innate immune responses in insects and suspected influence outcome malaria parasite infection mosquito vectors. Three Anopheles gambiae serpins (SRPN1, -2, -3) were tested for their ability inhibit prophenoloxidase cascade, a key regulatory process melanization response. Recombinant SRPN1 -2 can bind heterologous phenoloxidase-activating protease phenoloxidase activation vitro. Using reverse genetics approach, we studied effect SRPN2 on An....
We previously identified by genetic mapping an Anopheles gambiae chromosome region with strong influence over the outcome of malaria parasite infection in nature. Candidate gene studies interval, including functional tests using rodent Plasmodium berghei, a novel leucine-rich repeat gene, APL1, activity against P. berghei.Manual reannotation now reveals APL1 to be family at least 3 independently transcribed genes, APL1A, APL1B, and APL1C. Functional dissection indicates that among three...
The three-gene APL1 locus encodes essential components of the mosquito immune defense against malaria parasites. was originally identified because it lies within a mapped QTL conferring vector Anopheles gambiae natural resistance to human parasite, Plasmodium falciparum, and genes have subsequently been shown be involved in several species Plasmodium. Here, we examine molecular population genetic variation at gene cluster spatially temporally diverse West African collections A. gambiae. is...
Malaria remains a devastating disease despite efforts at control and prevention. Extensive studies using mostly rodent infection models reveal that successful Plasmodium parasite transmission by the African mosquito vector Anopheles gambiae depends on finely tuned vector-parasite interactions. Here we investigate transcriptional response of A. to geographically related falciparum populations various intensities different stages. These responses are compared with those mosquitoes infected...
Abstract Inversion polymorphisms are responsible for many ecologically important phenotypes and often found under balancing selection. However, the same features that ensure their large role in local adaptation—especially reduced recombination between alternate arrangements—mean uncovering precise loci within inversions control these is unachievable using standard mapping approaches. Here, we take advantage of long‐term selection on a pair mosquito Anopheles gambiae to map desiccation...