A5040879951- Malaria Research and Control
- Mosquito-borne diseases and control
- Breast Cancer Treatment Studies
- Vector-borne infectious diseases
- Agriculture and Rural Development Research
- Drug-Induced Hepatotoxicity and Protection
- Viral Infections and Vectors
- Agricultural safety and regulations
- Trypanosoma species research and implications
- Drug Transport and Resistance Mechanisms
- Travel-related health issues
- Computational Drug Discovery Methods
- Health, Medicine and Society
- Healthcare Systems and Practices
- Genetically Modified Organisms Research
- Research on Leishmaniasis Studies
- Traditional and Medicinal Uses of Annonaceae
- HIV/AIDS drug development and treatment
- Pharmacological Effects and Toxicity Studies
- Epilepsy research and treatment
- Social Sciences and Governance
- Vector-Borne Animal Diseases
- French Urban and Social Studies
- Hemoglobinopathies and Related Disorders
- Rabies epidemiology and control
Georgetown University
2021
Délégation Provence et Corse
2016-2018
Aix-Marseille Université
2003-2015
Institut de Médecine Tropicale du Service de Santé des Armées
2003-2012
Institut de Neurobiologie de la Méditerranée
2006-2011
Instituut voor Tropische Geneeskunde
1999-2011
Centre Hospitalier Universitaire de Bordeaux
2010
Galen University
2010
Victor (Japan)
2010
Armée de terre
2009
Abstract Basal and luminal are two molecular subtypes of breast cancer with opposite histoclinical features. We report a combined, high-resolution analysis genome copy number gene expression in primary basal cancers. First, we identified compared genomic alterations 45 48 tumors by using 244K oligonucleotide array comparative hybridization (aCGH). found various gains losses rare high-level amplifications that may provide therapeutic targets. show gain 10p is new alteration subregion the 8p12...
Malaria during the first pregnancy causes a high rate of fetal and neonatal death. The decreasing susceptibility subsequent pregnancies correlates with acquisition antibodies that block binding infected red cells to chondroitin sulfate A (CSA), receptor for parasites in placenta. Here we identify domain within particular Plasmodium falciparum erythrocyte membrane protein 1 binds CSA. We cloned var gene expressed CSA-binding parasitized blood (PRBCs). had eight receptor-like domains, each...
Abstract Background Parasite concentration methods facilitate molecular, biochemical and immunological research on the erythrocytic stages of Plasmodium . In this paper, an adaptation magnetic MACS ® columns for purification human species is presented. This method was useful concentration/purification either schizonts or gametocytes. Results conclusions The removal non-parasitized red blood cells (in vivo in vitro) using (MACS) evaluated. easy-to-use technique enriched gametocytes from...
We have cloned Pfnek-1, a gene encoding novel protein kinase from the human malaria parasite Plasmodium falciparum. This enzyme displays maximal homology to never-in-mitosis/Aspergillus (NIMA)/NIMA-like (Nek) family of kinases, whose members are involved in eukaryotic cell division processes. Similar other P. falciparum kinases and many enzymes NIMA/Nek family, Pfnek-1 possesses large C-terminal extension addition catalytic domain. Bacterially expressed recombinant is able autophosphorylate...
The cDNA encoding Pfmap-2, an enzyme of the human malaria parasite Plasmodium falciparum, was cloned, sequenced, and expressed in Escherichia coli. open reading frame carried by Pfmap-2 encodes a 508-amino acid polypeptide 59.2 kDa with maximal homology to mitogen-activated protein kinases (MAPKs) from various organisms. purified recombinant displayed functional characteristics MAPKs such as (i) ability undergo autophosphorylation, (ii) phosphorylate myelin basic protein, classical MAPK...
In Plasmodium falciparum , the causative agent of human malaria, catalytic subunit gene cAMP‐dependent protein kinase ( Pfpka‐c ) exists as a single copy. Interestingly, its expression appears developmentally regulated, being at higher levels in pathogenic asexualstages than sexual forms parasite that areresponsible for transmission to mosquito vector. Within asexual parasites, PfPKA activity can be readily detected schizonts. Similar endogenous PKA activityofnoninfected red blood cells,...
ABSTRACT The in vitro activities of cyclines (tetracycline, doxycycline, minocycline, oxytetracycline, and rolitetracycline), macrolides (erythromycin, spiramycin, roxithromycin, lincomycin), quinolones (norfloxacin ofloxacin), rifampin, thiamphenicol, tobramycin, metronidazole, vancomycin, phosphomycin, cephalosporins (cephalexin, cefaclor, cefamandole, cefuroxime, ceftriazone, cefotaxime, cefoxitin) were evaluated on Plasmodium falciparum clones, using an isotopic, micro-drug...
In the present study, we mapped major quantitative trait loci (QTL) differing between NZW and C57BL / 6 inbred strains of mice by making use (NZW × 6.Yaa)F1 mice, a model in which lupus-like autoimmune syndrome observed male is associated with presence an as yet unidentified Y chromosome-linked acceleration gene, Yaa. Linkage analysis 126 backcross males provided evidence for QTL on chromosome 7 controlling both severity glomerulonephritis production IgG anti-DNA autoantibody retroviral...
The Cd22 gene encodes a B cell-specific adhesion molecule that modulates cell Ag receptor-mediated signal transduction, and is allelic to lupus-susceptibility locus in New Zealand White (NZW) mice. In this study, we show that, addition the wild-type transcripts, NZW (Cd22a) mice synthesize aberrant CD22 mRNAs contain approximately 20-120 nucleotide insertions upstream of coding region between exons 2 3, and/or 100-190 deletions exon 4. Sequence analysis revealed these mRNA species arose by...
Abstract Background Plasmodium ovale is one of the five malaria species infecting humans. Recent data have shown that name this neglected masks two distinct genotypes also called curtisi and wallikeri . Some authors show these could be sympatric. These subspecies are not differentiated by microscopy techniques rapid diagnostic tests. This defect result low parasitaemia, antigenic polymorphism absence antibodies performance requires use sequencing techniques. An accurate easy discrimination...
In 2005, the Democratic Republic of Congo (DRC) switched to artesunate/amodiaquine as first-line antimalarial in response increasing sulfadoxine/pyrimethamine resistance and adopted intermittent preventive treatment using pregnancy.To determine prevalence molecular markers southwestern DRC 10 years after new policy was instituted.From March 2014 December 2015, blood samples were collected from symptomatic patients presenting outpatient centres urban rural areas. A total 2030 confirmed...
The in-vitro activities of pyronaridine, amodiaquine, chloroquine and quinine were evaluated against 161 isolates Plasmodium falciparum from Senegal (Dielmo, Ndiop Pikine), using an isotopic, micro, drug susceptibility test. mean IC50 values (50% inhibitory concentration) for pyronaridine amodiaquine 3.8 nM (95% confidence interval CI), 3.1-4.4) 12.0 CI, 10.0-14.0 nM), respectively. Pyronaridine more active than susceptible parasites. However, both drugs significantly less (P < 0.002 P...
Artesunate (ARS) is a water-soluble artemisinin derivative that potential alternative to quinine for the treatment of severe childhood malaria. We studied pharmacokinetics and bioavailability ARS given by intramuscular (i.m.) route in an open crossover study design. Fourteen children were randomized receive intravenous (i.v.) loading dose (2.4 mg/kg body weight) followed 12 h later i.m. (1.2 mg/kg) (group I), 14 i.v. II). carried out two-compartment analysis dihydroartemisinin (DHA;...
A total of 248 Plasmodium falciparum isolates were sampled in travelers with malaria who came to Marseille, France from Comoros investigate vitro activities antimalarial drugs and molecular markers drug resistance. Of the isolates, 126 maintained culture. these, 53% resistant chloroquine, 3% had reduced susceptibility quinine, mefloquine, atovaquone; 1% halofantrine dihydroartemisinin; 7% monodesethylamodiaquine; 37% cycloguanil; none lumefantrine. Resistance-associated point mutations...
For more than two years after the emergence of COVID-19 (Coronavirus Disease-2019), significant regional differences in morbidity persist. These clearly show lower incidence rates several regions African and Asian continents. The work reported here aimed to test hypothesis a pre-pandemic natural immunity acquired by some human populations central western Africa, which would, therefore, pose an original antigenic sin with virus antigenically close Severe Acute Respiratory Syndrome Coronavirus...
The in vitro activities of doxycycline, chloroquine, quinine, amodiaquine, artemether, pyrimethamine, and cycloguanil were evaluated against Plasmodium falciparum isolates from Senegal (Dielmo Ndiop), using an isotopic, micro, drug susceptibility test. 71-50% inhibitory concentration (IC50) values for doxycycline ranged 0.7 to 108.0 microM the geometric mean IC50 71 was 11.3 (95% confidence interval = 9.5-13.4 microM). activity did not differ significantly (P 0.0858) between...
Plasmodium falciparum , the major causative agent of human malaria, is an Apicomplexa protozoan parasite which invades in its intermediate host hepatocytes and erythrocytes. The driving force underlying internalization into cell thought to involve both polymerization actin, as entry inhibited by cytochalasins, actin motor‐associated protein. In related parasite, Toxoplasma gondii involvement during processes motility has been genetically established. a search for molecules that can regulate...
The in vitro activities of ferrochloroquine, chloroquine, quinine, mefloquine, halofantrine, amodiaquine, artesunate, atovaquone, cycloguanil and pyrimethamine were evaluated against Plasmodium falciparum isolates from Senegal (Dielmo, Ndiop), using an isotopic micro-drug susceptibility test. IC50 values for ferrochloroquine ranged 0.55 to 28.2 nM the geometric mean 55 was 7.9 (95% CI, 6.5-9.7 nM). Ferrochloroquine 35 times more active than chloroquine (35-fold greater chloroquine-resistant...
Real-time PCR diagnosis of malaria has advantages over traditional microscopic methods, especially when parasitaemia is low and dealing with mixed infections. We have developed a new real-time specific genes in each Plasmodium species present only one copy to identify the four pathogenic spp. for humans. The sensitivity was less than 25 parasites/microl. No cross-hybridisation observed human DNA or among Using LightCycler conventional microscopy, we compared patients from Vietnam returned...