A5074753644- Mycorrhizal Fungi and Plant Interactions
- Plant Pathogens and Fungal Diseases
- Forest Ecology and Biodiversity Studies
- Fungal Biology and Applications
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Lichen and fungal ecology
- Ecology and Vegetation Dynamics Studies
- Soil Carbon and Nitrogen Dynamics
- Microbial Community Ecology and Physiology
- Legume Nitrogen Fixing Symbiosis
- Plant Water Relations and Carbon Dynamics
- Yeasts and Rust Fungi Studies
- Cytokine Signaling Pathways and Interactions
- Microbial bioremediation and biosurfactants
- Genomics and Phylogenetic Studies
- NF-κB Signaling Pathways
- Plant and Fungal Species Descriptions
- Transgenic Plants and Applications
- T-cell and B-cell Immunology
- Forest ecology and management
- Anaerobic Digestion and Biogas Production
- Fungal and yeast genetics research
- Cephalopods and Marine Biology
- Biocrusts and Microbial Ecology
Agriculture and Agri-Food Canada
2017-2025
Ottawa Research and Development Centre
2020-2024
University of Ottawa
2023
Bridge University
2023
University of Lubumbashi
2023
Government of Canada
2022
Saskatoon Research and Development Centre
2020
Canadian Forest Service
2006-2018
Natural Resources Canada
2006-2018
Université de Montréal
2014-2016
Bioremediation is a cost-effective and sustainable approach for treating polluted soils, but our ability to improve on current bioremediation strategies depends isolate microorganisms from these soils. Although culturing widely used in research applications, it unknown whether the composition of cultured isolates closely mirrors indigenous microbial community contaminated To assess this, we paired culture-independent (454-pyrosequencing total soil DNA) with culture-dependent (isolation using...
Soil microbiome modification may alter system function, which enhance processes like bioremediation. In this study, we filled microcosms with gamma-irradiated soil that was reinoculated the initial or cultivated bacterial subsets obtained on regular media (REG-M) containing crude oil (CO-M). We allowed 8 weeks for stabilization, added and monoammonium phosphate, incubated another 6 weeks, then measured biodegradation of components, taxonomy, functional gene composition. hypothesized targeted...
Summary Rhizophagus irregularis is the model species for arbuscular mycorrhizal fungi (AMF) research and most widely propagated commercial plant biostimulants. Using asymbiotic symbiotic cultivation systems initiated from single spores, advanced microscopy, Sanger sequencing of glomalin gene, PacBio partial 45S rRNA we show that four strains R. produce spores two distinct morphotypes, one corresponding to morphotype described in protologue other having phenotype R . fasciculatus The spore...
Abstract Transposable elements are repetitive DNA sequences that excise or create copies inserted elsewhere in the genome. Their expansion shapes genome variability and evolution by impacting gene expression rearrangement rates. Arbuscular mycorrhizal fungi beneficial plant symbionts with large, transposable element-rich genomes, recent findings showed these vary significantly abundance, evolution, regulation among model strains. Here, we aimed to obtain a more comprehensive understanding of...
The Cd22 gene encodes a B cell-specific adhesion molecule that modulates cell Ag receptor-mediated signal transduction, and is allelic to lupus-susceptibility locus in New Zealand White (NZW) mice. In this study, we show that, addition the wild-type transcripts, NZW (Cd22a) mice synthesize aberrant CD22 mRNAs contain approximately 20-120 nucleotide insertions upstream of coding region between exons 2 3, and/or 100-190 deletions exon 4. Sequence analysis revealed these mRNA species arose by...
Phytoremediation is a potentially inexpensive alternative to chemical treatment of hydrocarbon-contaminated soils, but its success depends heavily on identifying factors that govern the root-associated microorganisms involved in hydrocarbon degradation and plant growth stimulation. Arbuscular mycorrhizal fungi (AMF) form symbioses with many terrestrial plants, are known stimulate growth, although both species identity environment influence this relationship. Although AMF suspected play role...
Arbuscular mycorrhizal fungi (AMF) are obligate plant symbionts that have the potential to improve crop yield. These multinucleate organisms either “homokaryotic” or “dikaryotic”. In AMF dikaryons, thousands of nuclei originating from two parental strains coexist in same cytoplasm. other fungi, homokaryotic and dikaryotic show distinct life history traits (LHTs), such as variation growth rates fitness. However, how compare between dikaryons homokaryons is unknown. To address this, we...
ABSTRACT The long-term impact of field-deployed genetically modified trees on soil mutualistic organisms is not well known. This study aimed at evaluating the poplars transformed with a binary vector containing selectable nptII marker and β-glucuronidase reporter genes ectomycorrhizal (EM) fungi 8 years after field deployment. We generated 2,229 fungal internal transcribed spacer (ITS) PCR products from 1,150 EM root tips 1,079 clones obtained organic mineral horizons within rhizosphere...
Armillaria possesses several intriguing characteristics that have inspired wide interest in understanding phylogenetic relationships within and among species of this genus. Nuclear ribosomal DNA sequence-based analyses provide only limited information for studies widely divergent taxa. More recent shown translation elongation factor 1-α (tef1) sequences are highly informative analysis diverse global regions. This study used Neighbor-net coalescence-based Bayesian to examine newly determined...
Wheat is among the important crops harnessed by humans whose breeding efforts resulted in a diversity of genotypes with contrasting traits. The goal this study was to determine whether different old and new cultivars durum wheat (Triticum turgidum L. var. durum) recruit specific arbuscular mycorrhizal (AM) fungal communities from indigenous AM populations soil under field conditions. A historical set five landraces 26 were cultivated humid climate Eastern Canada, phosphorus-limiting To...
Arbuscular mycorrhizal fungi (AMF) have been extensively studied in natural and agricultural ecosystems, but little is known about their diversity community structure highly petroleum-polluted soils. In this study, we described an unexpected of AMF a sedimentation basin former petrochemical plant, which petroleum hydrocarbon (PH) wastes were dumped for many decades. We used high-throughput PCR, cloning sequencing 18S rDNA to assess the molecular associated with Eleocharis obtusa Panicum...
The objective of this study was to investigate the foliar endophyte biodiversity white spruce ( Picea glauca (Moench) Voss establish a baseline for future comparative studies examining impact forestry practices. It identifies first time endophytic fungi living inside needles from 280 collected in seven natural stands southern Québec. colonization rate on surface sterilized 53.2%. We performed PCR-RFLP and sequence analysis ITS region nuclear ribosomal DNA achieve molecular identification....
For two decades, genetic engineering has made it possible to develop crops and trees designed for yield improvement simplified culture management. This, combined with field deployment of monocultures over large areas, can result in environmental stress unwanted potential side effects. The commercial production genetically modified (GM) the recent development GM raise concerns about their impact on environment, general, biodiversity non-target organisms, particular. Fungi are spread worldwide...
ABSTRACT The impact of transgenic white spruce [ Picea glauca (Moench) Voss] containing the endochitinase gene ( ech 42) on soil fungal biomass and ectendomycorrhizal fungi Wilcoxina spp. was tested using a greenhouse trial. measured level in roots up to 10 times higher than that nontransformed spruce. root exudates three four 42-transformed lines significantly greater controls. Analysis ergosterol showed amount samples from control slightly larger Nevertheless, difference not statistically...
Mechanisms involved in post-fire morel fructification remain unclear. A new undescribed belowground vegetative structure of Morchella tomentosa a burned boreal forest was investigated north Fairbanks, Alaska. The name "radiscisclerotium" is proposed to define this peculiar and elaborate below-ground M. tomentosa. Bayesian maximum parsimony analyses based on ITS rRNA regions nLSU gene strongly supported clade composed within the genus Morchella.
Chitinase genes isolated from plants, bacteria or fungi have been widely used in genetic engineering to enhance the resistance of crops and trees fungal pathogens. However, there are concerns about possible effect chitinase-transformed plants on nontarget fungi. This study aimed at evaluating impact endochitinase-transformed white spruce soil communities. Endochitinase-expressing untransformed controls were transplanted soils two natural forests grown for 8 months a greenhouse. Soil biomass...