A5019296339- Mycorrhizal Fungi and Plant Interactions
- Forest Ecology and Biodiversity Studies
- Legume Nitrogen Fixing Symbiosis
- Fungal Biology and Applications
- Plant Pathogens and Fungal Diseases
- Plant Disease Resistance and Genetics
- Nematode management and characterization studies
- Plant-Microbe Interactions and Immunity
- Cooperative Studies and Economics
- Plant and animal studies
- Yeasts and Rust Fungi Studies
- Plant and Fungal Species Descriptions
University of Ottawa
2017-2024
Summary Arbuscular mycorrhizal fungi ( AMF ) are known to improve plant fitness through the establishment of symbioses. Genetic and phenotypic variations among closely related isolates can significantly affect growth, but genomic changes underlying this variability unclear. To address issue, we improved genome assembly gene annotation model strain Rhizophagus irregularis DAOM 197198, compared its content with five R . sampled in same field. All harbor striking variations, large numbers...
Summary Chromosome folding links genome structure with gene function by generating distinct nuclear compartments and topologically associating domains. In mammals, these undergo preferential interactions regulate expression. However, their role in fungal biology is unclear. Here, we combine Nanopore (ONT) sequencing chromatin conformation capture (Hi‐C) to reveal chromosome epigenetic diversity a group of obligate plant symbionts: the arbuscular mycorrhizal fungi (AMF). We find that five...
The arbuscular mycorrhizal fungi (AMFs) are obligate root symbionts in the subphylum Glomeromycotina that can benefit land plants by increasing their soil nutrient uptake exchange for photosynthetically fixed carbon sources. To date, annotated genome data from representatives of AMF orders Glomerales, Diversisporales and Archaeosporales have shown these organisms large highly repeated genomes, no genes to produce sugars fatty acids. This led hypothesis most recent common ancestor (MRCA) was...
The root systems of most plant species are aided by the soil-foraging capacities symbiotic arbuscular mycorrhizal (AM) fungi Glomeromycotina subphylum. Despite recent advances in our knowledge ecology and molecular biology this mutualistic symbiosis, understanding AM genome is just emerging. Presented here a close to T2T assembly model fungus Rhizophagus irregularis DAOM197198, achieved through Nanopore long-read DNA sequencing Hi-C data. This haploid R. irregularis, alongside short-...
Abstract Transposable elements are repetitive DNA sequences that excise or create copies inserted elsewhere in the genome. Their expansion shapes genome variability and evolution by impacting gene expression rearrangement rates. Arbuscular mycorrhizal fungi beneficial plant symbionts with large, transposable element-rich genomes, recent findings showed these vary significantly abundance, evolution, regulation among model strains. Here, we aimed to obtain a more comprehensive understanding of...
Arbuscular mycorrhizal fungi (AMF) are a group of soil microorganisms that establish symbioses with the vast majority land plants. To date, generation AMF coding information has been limited to model genera grow well axenically; Rhizoglomus and Gigaspora. Meanwhile, data on functional gene repertoire most families is non-existent. Here, we provide primary large-scale transcriptome from eight poorly studied species (Acaulospora morrowiae, Diversispora versiforme, Scutellospora calospora,...
Abstract Arbuscular mycorrhizal fungi (AMF) are prominent root symbionts with a multinucleate cytoplasm that can carry thousands of nuclei deriving from two parental strains and varying in relative abundance large syncytium. Here, we set out to improve our understanding such remarkable genetics by resolving the nuclear genomes all publicly available AMF heterokaryons using PacBio HiFi Hi-C sequencing. We find sets homologous chromosomes, where genes associated plant colonization reside...
Abstract The root systems of most plant species are aided by the soil foraging capacities symbiotic Arbuscular Mycorrhizal (AM) fungi Glomeromycotina subphylum. Despite recent advances in our knowledge ecology and molecular biology this mutualistic symbiosis, understanding AM genome is just emerging. Presented here contiguous highest-quality nuclear mitochondrial assemblies an arbuscular mycorrhizal fungus to date, achieved through Nanopore long-read DNA sequencing Hi-C data. This haploid...
Abstract Transposable elements (TEs) are repetitive DNA sequences that excise or create copies inserted elsewhere in the genome. Their expansion shapes genome variability and evolution by impacting gene expression rearrangement rates. Arbuscular mycorrhizal fungi (AMF) beneficial plant symbionts with large, TE-rich genomes, recent findings showed these vary significantly abundance, evolution, regulation among model AMF strains. Here, we aimed to obtain a more comprehensive understanding of...
Abstract Chromosome folding links genome structure with gene function by generating distinct nuclear compartments and topologically associating domains (TADs). In mammals, these undergo preferential interactions regulate expression. However, their role in fungal biology is unclear. Here, we combine Nanopore (ONT) sequencing chromatin conformation capture (Hi-C) to reveal chromosome epigenetic diversity a group of obligate plant symbionts; the arbuscular mycorrhizal fungi (AMF). We find that...