A5080996637- Plant-Microbe Interactions and Immunity
- Mycotoxins in Agriculture and Food
- Plant Stress Responses and Tolerance
- Mycorrhizal Fungi and Plant Interactions
- Plant Pathogens and Fungal Diseases
- Plant Pathogenic Bacteria Studies
- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Forest Ecology and Biodiversity Studies
- CRISPR and Genetic Engineering
- Plant responses to water stress
- Plant Molecular Biology Research
- Genetically Modified Organisms Research
- Fungal Biology and Applications
- Polyamine Metabolism and Applications
- Evolution and Genetic Dynamics
- Microbial Natural Products and Biosynthesis
- 14-3-3 protein interactions
- Fungal and yeast genetics research
- Plant Genetic and Mutation Studies
- Polysaccharides and Plant Cell Walls
- Biochemical and Molecular Research
- Aeolian processes and effects
- Indoor Air Quality and Microbial Exposure
- Insect Resistance and Genetics
Agriculture and Agri-Food Canada
2017-2025
University of Ottawa
2023-2025
Ottawa Research and Development Centre
2018-2024
Cornell University
2013-2022
Ithaca College
2013-2022
Plant (United States)
2022
Communications Research Centre Canada
2019
International Development Research Centre
2019
University of Guelph
2010-2011
Molina Center for Energy and the Environment
2011
The balance between accumulation of stress-induced polyamines and reactive oxygen species (ROS) is arguably a critical factor in plant tolerance to salt stress. Polyamines are compounds, which accumulate plants under stress help maintain cellular ROS homeostasis. In this review we first outline the role mediating responses through their modulation redox two proposed roles regulating – as antioxidative molecules source synthesis discussed exemplified with recent studies. Second, function...
Deoxynivalenol (DON) is a mycotoxin virulence factor that promotes growth of the Fusarium graminearum fungus in wheat floral tissues. To further our understanding effects DON exposure on plant cell function, we characterized DON-induced transcriptional changes spikelets. Four hundred genes were differentially expressed during infection with wild-type F. as compared Δtri5 mutant strain unable to produce DON. Most these more induced by DON-producing and included involved secondary metabolism,...
Cytosolic glutamine synthetase (GS1) is responsible for the primary assimilation of ammonia, and a role in nitrogen (N) remobilization implicated from its vascular localization enhanced expression during senescence. This paper tested hypothesis that overexpression (OX) GS1 rice improves utilization N use efficiency (UtE = spikelet yield/shoot content). Three OX lines were identified using activity assays quantitative polymerase chain reaction. Physiological analysis lines, as well azygous...
Plant perception of pathogen-associated molecular patterns (PAMPs) and other environmental stresses trigger transient ion fluxes at the plasma membrane. Apart from role Ca2+ uptake in signaling, regulation significance PAMP-induced immunity remain unknown. We characterized functions INTEGRIN-LINKED KINASE1 (ILK1) that encodes a Raf-like MAP2K kinase with insufficiently understood plants. Analysis ILK1 mutants impaired expression or activity revealed contributes to plant defense bacterial...
Diverse plant species detect bacterial flagellin using leucine-rich repeat receptor-like kinases (LRR RLKs) to activate defense responses and promote resistance against invasion. In barley, the impact of on inducible has not been fully described. Here, we report that flagellin-derived flg22 peptide induces callose, ROS bursts differential expression 2603 genes in barley. Cultivated barley genotypes produce different amplitudes flg22-induced indicating genetic variation for MAMP responses....
The enzyme phytochelatin synthase (PCS) has long been studied with regard to its role in metal(loid) detoxification several organisms, i.e., plants, yeasts and nematodes. It is fact widely recognized that PCS detoxifies a number of heavy metals by catalysing the formation thiol-rich oligomers, namely phytochelatins, from glutathione related peptides. However, recent investigations have highlighted other possible roles played plant cell, e.g. control pathogen-triggered callose deposition. In...
The virulence and broad host range of Fusarium graminearum is associated with its ability to secrete an arsenal phytotoxic secondary metabolites, including the regulated mycotoxins belonging deoxynivalenol family. TRI genes responsible for biosynthesis related compounds are usually expressed during fungal infection. However, F. genome harbors array unexplored biosynthetic gene clusters that also co-induced genes, nonribosomal peptide synthetase 8 (NRPS8) cluster. Here, we identify two...
Abstract Background Targeted genome editing using the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system has been applied in a large number of plant species. Using gene-specific single guide RNA (sgRNA) and CRISPR/Cas9 system, small events such as deletions few bases can be obtained. However larger are required for some applications. In addition, identification characterization edited challenging plants with complex genomes, wheat. Results this study, we used...
Abstract Arbuscular mycorrhizal fungi (AMF) are prominent root symbionts with a multinucleate cytoplasm that can carry thousands of nuclei deriving from two parental strains and varying in relative abundance large syncytium. Here, we set out to improve our understanding such remarkable genetics by resolving the nuclear genomes all publicly available AMF heterokaryons using PacBio HiFi Hi-C sequencing. We find sets homologous chromosomes, where genes associated plant colonization reside...
There is considerable confusion about N use efficiency (NUE) in the plant literature. We would like to propose simple and ubiquitous definitions described by Good et al. (2004) as a starting point for studies of NUE. Based on this terminology, there evidence from breeding programs variation both uptake (UpE) utilization (UtE). Molecular physiology typically address mechanisms improving NUE, but often do not calculate NUE or even acquire appropriate data calculating Herein, we report detail...
Protein kinase–driven phosphorylation constitutes the core of cellular signaling. Kinase components signal transduction pathways are often targeted for inactivation by pathogens. The study kinases and immune in model crop tomato (Solanum lycopersicum) would benefit from availability community-wide resources large scale systems-level experimentation. Here, we defined kinome performed a comprehensive comparative analysis 15 other plant species. We constructed kinase library (TOKN 1.0) over 300...
Kinases facilitate detection of extracellular signals and set in motion cellular responses for plant adaptation survival. Some the energy utilized kinase signal processing is produced through activity ion transporters. Additionally, synergy between ions transduction influences response to pathogens, their growth development. In plants, signaling elements that connect cell wall membrane sensors with homeostasis transport-mediated processes are largely unknown. Current research indicates...
Fungal pathogens survive harsh environments and overcome physical, temporal, chemical barriers to colonize their hosts reproduce. Fusarium graminearum was one of the first fungal plant for which transcriptomic tools were developed, making analysis gene expression a cornerstone approach in studying its biology. The diverse vitro conditions during infection different cereal crops has revealed subsets both unique shared transcriptionally regulated genes. Together with genetic studies, these...
Barley is the third most important cereal crop in terms of production Canada, and Fusarium head blight (FHB) one main fungal diseases barley. FHB caused by a species complex Fusaria, which graminearum Schwabe causal epidemics Canada. Field surveys show that two or more often co-exist within same field grain sample, F. poae reported as another This study aimed to determine pathogenicity graminearum, poae, co-inoculation both causing Two susceptible barley cultivars were spray-inoculated at 10...
Plants perceive externally produced microbe-associated molecular patterns (MAMPs) and endogenously danger-associated (DAMPs) to activate inducible immunity. While several immune responses have been observed during Fusarium graminearum infection, the identity of signaling pathways involved is only partly known. We screened 227 receptor kinase innate response genes in Arabidopsis identify nine with a role F. resistance. Resistance-promoting included chitin receptors LYK5 CERK1, reactive oxygen...
Plant protein kinases form redundant signaling pathways to perceive microbial pathogens and activate immunity. Bacterial repress cellular immune responses by secreting effectors, some of which bind inhibit multiple host kinases. To understand how broadly bacterial effectors may the function these kinase interactors, we first tested kinase–effector (K-E) interactions using Pseudomonas syringae pv. tomato–tomato pathosystem. We between five individual (HopAI1, AvrPto, HopA1, HopM1, HopAF1) 279...
A Report on the Plant Genomes & Biotechnology: From Genes to Networks meeting, held at Cold Spring Harbor Laboratories, USA, December 4-7, 2013.
Pseudokinases are thought to lack phosphotransfer activity due altered canonical catalytic residues within their kinase domain. However, a subset of pseudokinases maintain through atypical mechanisms. The Arabidopsis ILK1 is pseudokinase from the Raf-like MAP3K family and only known plant with confirmed protein activity. promotes disease resistance molecular pattern-induced root growth inhibition its stabilization HAK5 potassium transporter calmodulin-like CML9. also has kinase-independent...
Microbial plant pathogens deploy amphipathic cyclic lipopeptides to reduce surface tension in their environment. While plants can detect these molecules activate cellular stress responses, the role of or associated host responses pathogenesis are not fully clear. The gramillin lipopeptide is produced by Fusarium graminearum fungus and a virulence factor toxin maize. Here, we show that promotes necrosis both monocots dicots disrupting ion balance across membranes. Gramillin cation-conducting...
Abstract Low seed dormancy is an essential trait in malting barley since involves rapid and uniform induction of germination. At least two major QTLs on chromosome 5H, SD1 SD2, regulate multiple populations, additional genetic regions are thought to be involved. To identify novel loci that might associated with dormancy, a panel genotypes from diverse agro-ecosystems were evaluated alongside Canadian biparental populations for germination rate. Association mapping revealed within the SD2...